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101.
Merino S Domènech O Díez-Pérez I Sanz F Montero MT Hernández-Borrell J 《Colloids and surfaces. B, Biointerfaces》2005,44(2-3):93-98
Atomic force microscopy (AFM) was used to study the influence of a membrane protein, lactose permease of Escherichia coli (LacY), on the surface spreading behavior and the features of self-assembled phospholipids bilayers on mica. The miscibility of phospholipids used, 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), was investigated by surface pressure area isotherm measurements at the air-water interface. A composition with an equimolar proportion of POPC and DMPC was used to form the liposomes. Surface layers formed with DMPC:POPC (0.5:0.5, mol/mol) or LacY reconstituted in proteoliposomes with the same phospholipid composition were imaged by using AFM. When lactose permease was reconstituted in DMPC:POPC (0.5:0.5, mol/mol), self-assembled structures that remained firmly adsorbed onto the mica surface were observed. These sheets had an irregular shape and their upper layer was more corrugated than that obtained for the phospholipid matrix. 相似文献
102.
Journal of Algebraic Combinatorics - Given any irreducible Coxeter group C of hyperbolic type with nonlinear diagram and rank at least 4, whose maximal parabolic subgroups are finite, we construct... 相似文献
103.
Raine Garrido Arteaga Felix Cardoso San Jorge María del C. Rodríguez Montero Vicente Vérez Bencomo Herman Vélez Castro 《Magnetic resonance in chemistry : MRC》2012,50(8):525-528
Quimi‐Hib is a conjugate vaccine against Haemophilus influenza type b (Hib) where the Hib antigen is the only one produced by chemical synthesis. NMR has become the alternative of choice for the identity of intermediates during the chemical synthesis of Hib antigen. We explore a rapid quantitative proton magnetic resonance (qHNMR) assay for the determination of N,N‐dimethylformamide (DMF) as a residual in one of the critical intermediates. The proposed assay has been shown to be accurate, precise for intermediate precision conditions (relative standard deviation <3% for spectrometer‐to‐spectrometer variations), specific (no detected interferences), and rugged (percentage difference <3% for day‐to‐day and spectrometer‐to‐spectrometer variations). The quantitative NMR assay can replace the common chromatographic methods for monitoring the DMF contents in one crucial step of the synthetic scheme. Copyright © 2012 John Wiley & Sons, Ltd. 相似文献
104.
Luis A. Montero Jos Molina Jürgen Fabian 《International journal of quantum chemistry》2000,79(1):8-16
Multiple minima of water cluster hypersurfaces are explored to find thermodynamic properties by means of the corresponding partition functions of their canonical distributions. The combination of semiempirical quantum chemical procedures for calculating the cluster energies in local minima of supermolecules and the statistical thermodynamics approach for both the evaluation of macroscopic association energies and the possible reduction by average of absolute errors intrinsic to the parametrized Hamiltonian are shown to provide an appropriate model for comparison between experimental and theoretical results. The method can explicitly take into account environmental effects due to intermolecular interaction. Water trimer and tetramer association energies of ?10.9 and ?14.1 kJ/mol obtained from virial coefficient calculations compare very well to the values of ?10.5 and ?16.4 kJ/mol, respectively, found for the theoretical association energies in this paper. © 2000 John Wiley & Sons, Inc. Int J Quant Chem 79: 8–16, 2000 相似文献
105.
van Pinxteren MS Montero L Jäsch S Paschke H Popp P 《Analytical and bioanalytical chemistry》2009,393(2):767-775
A new approach for headspace sorptive extraction is presented and demonstrated for the determination of 12 chlorobenzenes
in water samples. It consists of a silicone tube (15-mm length) arranged around a stainless steel rod. This device is fixed
on a septum cap and exposed to the headspace of 50 mL of a salt-saturated water sample. After extraction (60-min optimized
extraction time), thermodesorption is carried out by direct insertion of the silicone tube into the thermodesorption-gas chromatography-mass
spectrometry system. Desorption of the analytes is performed at 250 °C for 5 min with a gas flow of 50 mL/min. Repeatability
(relative standard deviation 5–10%), extraction yields (9–46%), enrichment factors (129–657), and detection limits (0.002–0.012 μg/L)
were determined and four real water samples were analyzed with the headspace tube extraction. The results were verified by
standard addition. A comparison of headspace tube extraction with other headspace enrichment techniques underlined the high
extraction capacity of the proposed method. A big advantage of tube extraction is the low cost of the silicone material. The
tubes can be discarded after single use, avoiding carryover problems and cross-contamination.
Figure Scheme of the HS-tube extraction and thermodesorption system 相似文献
106.
Doménech O Merino-Montero S Montero MT Hernández-Borrell J 《Colloids and surfaces. B, Biointerfaces》2006,47(1):102-106
In this work, using atomic force microscopy (AFM), we have studied the influence of the temperature on the properties of the surface planar bilayers (SPBs) formed with: (i) the total lipid extract of Escherichia coli; (ii) 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (DMPC) (1:1, mol/mol); and, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanol-amine (POPE) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol (POPG) (3:1, mol/mol). According to the height profile analysis we performed, the height of the SPBs of DMPC:POPC were temperature dependent. Separated domains were observed in the SPBs of the POPE:POPG mixture and the E. coli lipid extract. The implication of those domains for the correct insertion of membrane proteins into proteoliposomes is discussed. 相似文献
107.
Alvaro M Carbonell E Ferrer B Garcia H Herance JR 《Photochemistry and photobiology》2006,82(1):185-190
Ionic liquids are suitable media which stabilize charged intermediates favoring those mechanisms that occur through charge separation. We have used ionic liquids to develop a photocatalytic system to perform the reduction of a carbonyl group to alcohol, thus mimicking the behavior of the reductase enzymes. The photochemical cycle is based on the well-known electron transfer from the Ru(bpy)(3)2+ complex in its excited state, acting as electron donor to MV2+, which acts as electron acceptor. The initial electron transfer process can be promoted upon selective Ru(bpy)(3)2+ excitation by visible light. By means of laser flash photolysis we have provided evidence of the nature and lifetimes of the intermediates involved in the photocatalytic system. Thus, the initial electron transfer between Ru(bpy)(3)2+ triplets and viologen MV2+ forms the MV*+ radical cation, which upon accepting an H* atom from a suitable hydrogen atom donor, forms the corresponding dihydropyridine MVH+ reducing agent. 相似文献
108.
The different parameters affecting the ionisation and fragmentation of selected polychlorinated biphenyls (PCBs) in an IT detector working in the MS/MS mode, ITD(MS/MS), have been optimised for maximum selectivity and sensibility. The low LODs (in the range 0.03-0.3 microg/L), the satisfactory repeatability (RSDs in general below 11%) and reproducibility (RSDs below 17%) obtained when analysing standard solutions ensured proper determination of the PCBs studied at the concentrations typically found in food samples. Foodstuffs naturally contaminated with varying levels of PCBs have been analysed using the optimised GC-ITD(MS/MS) method. The results obtained compared favourably with those found using more conventional detectors, such as (micro-)electron capture detection (for ortho-PCBs) and high-resolution MS (for non-ortho-PCBs), as well as with the consensus PCB levels established for these particular samples via an international interlaboratory exercise. The relative merits of these three detectors have been discussed. 相似文献
109.
This paper presents a theoretical analysis of a density measurement cell using an unidimensional model composed by acoustic and electroacoustic transmission lines in order to simulate non-ideal effects. The model is implemented using matrix operations, and is used to design the cell considering its geometry, materials used in sensor assembly, range of liquid sample properties and signal analysis techniques. The sensor performance in non-ideal conditions is studied, considering the thicknesses of adhesive and metallization layers, and the effect of residue of liquid sample which can impregnate on the sample chamber surfaces. These layers are taken into account in the model, and their effects are compensated to reduce the error on density measurement. The results show the contribution of residue layer thickness to density error and its behavior when two signal analysis methods are used. 相似文献
110.