排序方式: 共有72条查询结果,搜索用时 109 毫秒
41.
42.
43.
44.
A fast method for the generation of permanent hydrophilic capillary coatings for capillary electrophoresis (CE) is presented. Such interior coating is effected by treating the surface to be coated with a solution of glutaraldehyde as cross-linking agent followed by a solution of poly(vinyl alcohol) (PVA), which results in an immobilization of the polymer on the capillary surface. Applied for capillary zone electrophoresis (CZE) such capillaries coated with cross-linked PVA exhibit excellent separation performance of adsorptive analytes like basic proteins due to the reduction of analyte-wall interactions. The long-term stability of cross-linked PVA coatings could be proved in very long series of CZE separations. More than 1000 repetitive CE separations of basic proteins were performed with stable absolute migration times relative standard deviation (RSD > 1.2%) and without loss of separation efficiency. Cross-linked PVA coatings exhibit a suppressed electroosmotic flow and excellent stability over a wide pH range. 相似文献
45.
Stefan Jezierski Anke S. Klein Christian Benz Michael Schaefer Stefan Nagl Detlev Belder 《Analytical and bioanalytical chemistry》2013,405(16):5381-5386
We immobilized adherent human embryonic kidney (HEK) cells—which are able to trace adenosine triphosphate (ATP) —inside a microfluidic free-flow electrophoresis (μFFE) chip in order to develop an integrated device combining separation and biosensing capabilities. HEK 293 cells loaded with fluorescent calcium indicators were used as a model system to enable the spatially and temporally resolved detection of ATP. The local position of a 20 μM ATP stream was successfully visualized by these cells during free-flow electrophoresis, demonstrating the on-line detection capability of this technique towards native, unlabeled compounds. 相似文献
46.
M. Sc. Christian Benz Dr. Michael Boomhoff M. Sc. Johannes Appun Prof. Dr. Christoph Schneider Prof. Dr. Detlev Belder 《Angewandte Chemie (International ed. in English)》2015,54(9):2766-2770
Free‐flow electrophoresis is an ideal tool for preparative separations in continuous microflow. With the approach presented herein for coupling free‐flow electrophoresis and mass spectrometry it is now also possible to trace non‐fluorescent compounds and identify them by means of mass spectrometry. The functionality of the method and its potential as an integrated separation unit for microflow synthesis is demonstrated by application to a multicomponent [3+2]‐cycloannulation. 相似文献
47.
48.
49.
50.
Belder D 《Angewandte Chemie (International ed. in English)》2005,44(23):3521-3522