Accurate measurement of long-range heteronuclear coupling constants from undistorted multiplets of an enhanced CPMG-HSQMBC experiment

Here, we present a modified CPMG-HSQMBC experiment which is capable to reduce the detrimental phase twists in the "long-range" connectivity multiplets caused by proton-proton couplings. We demonstrate that concerted CPMG pulse trains applied on both nuclei in the starting CPMG-INEPT transfer step can considerably be improved by composite pi pulses that compensate for pulse imperfections and off-resonance effects. Experimental optimization of the interpulse delay within the CPMG cycle was found to be crucial in order to achieve the best possible "decoupling" of homonuclear coupling modulation.     

New synthesis of oxindole‐1‐carboxamides

A new synthesis of oxindole‐1‐carboxamides was elaborated by the reaction of oxindole‐1‐phenyl‐carboxylate with various amines. This method also permitted the preparation of N,N‐disubstituted oxindole‐1‐carboxamides.     

Fourier Transform of Symmetric Gauss Measures on the Heisenberg Group

An explicit form is derived for the Fourier transform of symmetric Gauss measures on the Heisenberg group at the Schrödinger representation. Using this explicit formula, necessary and sufficient conditions are given for the convolution of two symmetric Gauss measures to be a symmetric Gauss measure and for commutability of two symmetric Gauss measures. Moreover, necessary and sufficient conditions are presented for the convolution of two symmetric Gauss convolution semigroups to be a convolution semigroup. May 8, 2001     

Well Ordering Groups with no Monotone Arithmetic Progressions

Károlyi–Kós and Ardal–Brown–Jungic proved that every vector space over \(\mathbb {Q}\) has an ordering with no monotone three term arithmetic progression (3-AP). We show that every solvable group has a well ordering with no monotone 6-AP, and each hypoabelian group has an ordering omitting monotone 5-APs. Finally, we prove that every group has a well ordering with no infinite monotone AP.     

Microanalytical method development for Fe, Cu and Zn determination in colorectal cancer cells

Microanalytical methods suitable for the determination of Fe, Cu in HT-29 (human colon adenocarcinoma) cells treated with different iron compounds (Fe(II) sulfate, Fe(III) chloride, Fe(III) citrate and Fe(III) transferrin) and cultured in medium supplemented or not with 10% (v/v) fetal calf serum (FCS) by total reflection X-ray fluorescence spectrometry (TXRF) and simultaneous graphite furnace atomic absorption spectrometry (GF-AAS) were developed. The developed TXRF method was also suitable for Zn determination in the samples. The main advantage of the proposed methods is the execution of all sample preparation steps following incubation and prior to the elemental analysis in the same Eppendorf tubes. Sample preparation was performed at microscale (115 μL sample volume) with 65% nitric acid and 30% hydrogen peroxide. According to scanning electron microscopic measurements, the organic matrix of the cell samples could be eliminated to the extent that accurate results were obtained for Cu and Fe by analyzing the same samples by TXRF and GF-AAS. Concerning the iron uptake, HT-29 cells incubated in FCS-free medium contained Fe in cca. 5-50 times higher amounts compared to cells cultured in FCS supplemented medium. Pronounced differences in the iron uptake compared to the iron supply (inorganic vs. organic chelated as well as iron(II) vs. iron(III)) were observed in the case of cell lines incubated in FCS-free medium.     

Halogen

Ohne Zusammenfassung     

Synthesis of isoquinobenzodiazepinediones

1-(2′-Chloroacetylamino)-4,4-dimethyl-1,4-dihydro-3(2H)-isoquinolinone ( 3 ) was cyclised by treatment with sodium hydride in dimethyl sulphoxide containing 0.1 % of water to give 10,10-dimethyl-6,7,9,10-tetrahydro-5H,14bH-isoquino[2,1-d][1,4]benzodiazepine-6,9-dione ( 4 ) in a yield of 80%. In anhydrous dimethyl sulphoxide the main product of the reaction was 5-N-(4,4-dimethyl-1-phenyl-1,4-dihydro-3(2H)-isoquinolinon-1′-yl)isoquino[2,1-d][1,4]benzodiazepine-6,9-dione ( 5 ), which was also prepared by the reaction of 3 with 4 .     

Konvexes Polyeder als geometrischer Ort

Ohne Zusammenfassung Aus dem Nachlaß des Verfassers bearbeitet durch L. Fejes Tóth