Fluorescence methods to study lipid-protein association: The interaction of protein kinase C with lipid-loaded mixed micelles

The interaction of protein kinase C with lipids was studied in a mixed micellar system. Two fluorescence spectroscopic methods are presented with a different but complementary information content. Diffusion monitored by fluorescence correlation spectroscopy provides information on the interaction of the protein with the whole lipid aggregate. Resonance energy transfer from tryptophans to pyrene-labeled lipids monitored by time-correlated single-photon counting supplies information on the interaction of the protein with specific lipid cofactors within the micelle. The results can be extended to postulate new mechanisms for the activation of protein kinase C by the signal transduction cascades in the cell. Both fluorescence spectroscopic methods can be easily applied to other protein systems which interact with lipids.     

Synthesis and Crystal Structure of N,N′-4-aminomethyl-pyromellitic diimide

The title compound N,N′-4-aminomethyl-pyromellitic diimide (4-pmpmd) crystallizes in monoclinic, space group P21/c with a = 4.785(1), b = 6.200(1), c = 29.907(2) (A), β =93.583(11)°, V= 885.5(2) (A)3, Dc = 1.494 g/cm3, Z = 2, C22H14N4O4, Mr = 398.37,μ(MoKα) = 0.106mm-1 and F(000) = 412, and its structure was refined to R = 0.0469 and wR = 0.1017 for 1194observed reflections (Fo ＞ 4σ(Fo)). The X-ray diffraction shows the existence of the staggered multi-dimensional supramolecular network based on the Z-mode conformation of the title compound.     

从FeSe0.5Te0.5的V～I曲线论磁通动力学的诸模型（英文）

高温超导体的发现动摇了支配磁通动力学二十几年的Kim-Anderson模型.于是人们提出了新的涡旋玻璃态模型,集体钉扎模型和U～j对数关系模型来描述高温超导体的磁通动力学.其中,涡旋玻璃态模型已被人们广泛接受.Fe基超导体发现后,人们它行为十分类似于高温超导体.我们实验上仔细研究了FeSe0.5Te0.5单晶的V～I关系曲线,发现上述诸模型均不能很好的解释我们的实验结果,而张的反跳模型恰能很好的拟合实验结果.本文讨论了诸模型对高温超导体磁通动力学描述的适用性并给出合理的解释.     

Cooling of Akmal-Pandharipande-Ravenhall neutron stars with a rotochemical heating source

Employing phenomenological density-dependent critical temperatures of strong singlet-state proton pairing and of moderate triplet-state neutron pairing, we investigate the effects of rotochemical heating on the thermal evolution of superfluid neutron stars whose cores consist of npe matter with the Akmal-Pandharipande-Ravenhall equation of state. Since the star is not quite in the weak interaction equilibrium state during spin-down, the departure from the chemical equilibrium leads to the rotochemical heating in a rotating NS which will increase the stellar＇s temperature. Our calculations show that the rotochemical heating delays the cooling of superfluid neutron stars considerably and makes the previous classification of NS cooling ambiguous. What＇s more, our model is currently consistent with all the observational data, and in particular some middle-aged and cold NSs （PRS J0205＋6449 in 3C 58, PRS J1357-6429, RX J007.0＋7303 in CTA 1, Vela） can be better explained when taking into account rotochemical heating.     

Cooling of Akmal-Pandharipande-Ravenhall neutron stars with a rotochemical heating source

Employing phenomenological density-dependent critical temperatures of strong singlet-state proton pairing and of moderate triplet-state neutron pairing, we investigate the effects of rotochemical heating on the thermal evolution of superfluid neutron stars whose cores consist of npe matter with the Akmal-Pandharipande-Ravenhall equation of state. Since the star is not quite in the weak interaction equilibrium state during spin-down, the departure from the chemical equilibrium leads to the rotochemical heating in a rotating NS which will increase the stellar's temperature. Our calculations show that the rotochemical heating delays the cooling of superfluid neutron stars considerably and makes the previous classification of NS cooling ambiguous. What's more, our model is currently consistent with all the observational data, and in particular some middle-aged and cold NSs (PRS J0205+6449 in 3C 58, PRS J1357-6429, RX J007.0+7303 in CTA 1, Vela) can be better explained when taking into account rotochemical heating.     

超声乳化溶剂扩散法制备阿奇霉素超细粉体

采用超声乳化溶剂扩散法制备了阿奇霉素超细粉体，讨论了溶剂、温度、阿奇霉素浓度以及稳定剂种类和用量等条件对微粉粒径及形貌的影响．实验结果表明，在实验选定的最佳实验条件下(结晶温度0～5℃，阿奇霉素浓度0．03mol／L，1％聚乙二醇(PEG)、乙醇为溶剂)，可制备出平均粒径为500nm的均匀分散阿奇霉素超细粉体。     

Synthesis and Crystal Structure of N,N′-4-aminomethyl-pyromellitic diimide

1 INTRODUCTION N,N?-4-aminomethyl-pyromellitic diimide (4-pmpmdwas firstly obtained[1] in 1989 as one of the bypro-ducts by refluxing equimolar quantities of pyrome-llitic anhydride, n-hexylamine and 4-aminomethyl py-ridine in dry DMF to assemble electronic donor-spa-cer-acceptor systems, but its structure and coordina-tion chemistry did not receive attention at that time.For 4-pmpmd, the entity of two methylene groups(-CH2-) between the rigid pyromellitic diimide (PMDcore and two fre…     

富氧燃烧条件下煤焦的燃尽特性

本文利用平面火焰携带流反应器研究了DT烟煤在富氧燃烧条件下的燃烧实验。采用灰示踪法分析煤焦的燃尽和元素释放特性,并采用等密度模型计算了基于氧化反应C+0.5O_2→CO的表观反应动力学参数。研究结果表明;煤粉在富氧燃烧条件下的燃尽慢于空气燃烧;富氧燃烧条件下,煤焦与CO_2的气化反应会导致煤焦表面对O的化学吸附,进而导致氧元素释放速率减慢;高氧浓度条件下,高浓度CO_2对煤焦燃尽的抑制作用大于CO_2气化反应对煤焦燃尽的促进作用,降低环境氧浓度可以逐步提高CO_2气化反应对煤焦燃尽的贡献。     

LPL3层膜的结构、磁性和磁电阻效应的研究

采用直流溅射法在 ( 1 0 0 )LaAlO3 单晶基片上制备了La_0.67Sr_0.33MnO_3+δ/Pr_0.7Ca_0.3MnO_3+δ/La_0.67Sr_0.33MnO_3+δ(简称为LPL) 3层膜 .用X射线粉末衍射法(XRD)研究了系列样品的摇摆曲线和衍射全图 ,结果表明所有的样品均为高度取向的外延膜 .SQUID磁强计的测量结果证实了 3层膜中磁耦合的存在 .用常规的四端引线法测量了LSMO、PCMO和LPL 3层膜的电阻 ,分析了logρ 1 /T曲线 .由此可以得出如下结论 :具有铁磁性的PCMO中间层在 3层膜中可能起到了内磁场的作用 ,使得LSMO膜的顺磁性被削弱 ,这个作用与外加磁场的作用一样 ,降低了 ρ_max,增大了由金属到半导体的转变温度T_p;PCMO中间层还诱发了LSMO的能隙中的态密度的变化 .以上两个原因使得在零场中样品的电阻率和T_p 随着中间层PCMO的厚度变化而明显变化 .     

多态性及基因测序分析方法研究苯丙酮尿症患者的基因突变

应用聚合酶链反应-单链构象多态性分析及基因测序的方法,分析35例苯丙酮尿症患者PAH基因第6和7外显子以及其两侧部分内含子序列,进行了突变的筛查和确定.研究吉林地区苯丙酮尿症人群中苯丙氨酸羟化酶(Phenylalanine hydroxylase,PAH)基因突变特征.结果显示:(1)在70个PAH等位基因中共检测出9...