High-tech breakthrough DNA scanner for reading sequence and detecting gene mutation

The 17″×14″ X-ray film, gels, and blots are widely used in DNA research. However, DNA laser scanners are costly and unaffordable for the majority of surveyed biotech scientists who need it. The high-tech breakthrough analytical personal scanner (PS) presented in this report is an inexpensive 1 lb hand-held scanner priced at 2–4% of the bulky and costly 30–95 lb conventional laser scanners. This PS scanner is affordable from an operation budget and biotechnologists, who originate most science breakthroughs, can acquire it to enhance their speed, accuracy, and productivity. Compared to conventional laser scanners that are currently available only through hard-to-get capital-equipment budgets, the new PS scanner offers improved spatial resolution of 20 μm, higher speed (scan up to 17″×14″ molecular X-ray film in 48 s), 1–32,768 gray levels (16-bits), student routines, versatility, and, most important affordability. Its programs image the film, read DNA sequences automatically, and detect gene mutation. In parallel to the wide laboratory use of PC computers instead of mainframes, this PS scanner might become an integral part of a PC-PS powerful and cost-effective system where the PS performs the digital imaging and the PC acts on the data.     

Synthesis of 1,7-bis(4′-aminophenylamido)-m-carborane and 1,7-bis(4′-aminophenylcarboxy)-m-carborane

Conclusion 1,7-Bis(4-aminophenylamido)- and l,7-bis(4-aminophenylcarboxy)-m-carboranes were prepared and characterized.Translated from Izvestiya Akademii Nauk SSSR, Seriya Khimicheskaya, No. 12, pp. 2786–2787, December, 1986.     

Lactam and acid amide acetals. 71. New synthesis of 3-nitro-6-hydroxyindole derivatives

Previously unknown 3-nitro-6-hydroxyindole derivatives were synthesized by condensation of p-benzoquinone with secondary -nitro enamines.See [1] for Communication 70.Translated from Khimiya Geterotsiklicheskikh Soedinenii, No. 1, pp. 40–45, January, 1992.     

Catalytic hydroamination of furfuryl and tetrahydrofurfuryl alcohols with nitriles

Reaction of furfuryl and tetrahydrofurfuryl alcohols with nitriles over copper oxide catalysts under a hydrogen pressure of 15 atm at a temperature of 230C gives N-alkylfurfuryl- (yield 46–50%) and N-alkyl-tetrahydrofurfurylamines (49–53%), and N-alkylpiperidines (28–41%). A reaction mechanism is proposed.Translated from Khimiya Geterotsiklicheskikh Soedinenii, No. 1, pp. 23–26, January, 1988.     

Thermochemical reactions in polycomplexes

Polycomplex formation between poly(methacrylic acid) and polyacrylamide in aqueous solutions has been studied by means of viscometry, potentionetry and elemental analysis. Stoichiometry of the polycomplex was found to be 1:1 with respect to unit-moles. I.R.-spectroscopic data give evidence of the co-existence of ladder-like regions and loops in the polycomplexes formed by poly(methacrylic acid) with polyacrylamide and poly(ethylene glycol). The reactivities of the same functional groups in ladder-like regions and loops are different. This difference has been shown taking as an example thermochemical reactions in the above polycomplexes. Dehydrocyclization in the poly(methacrylic acid) chain was found to occur in the loops of both polycomplexes. This intramolecular reaction in the polycomplex of poly(methacrylic acid) with polyacrylamide is followed by intermolecular formation of imide bonds in ladder-like regions.