Isolation and Purification of Recombinant HBsAg of Human Hepatitis B Virus from Silkworm Larvae

Recombinant HBsAg coded by preS1-preS2-S regions of hepatitis B virus was expressed in Bombyx mori silkworm larvae. Recombinant HBsAg was expressed (30–40 μg/mL, 0.1% of the total amount of extracted protein) by larvae infected with recombinant baculovirus rBmNPV-Hep-preS1-S containing cDNA of HBsAg strictly by the polyhedrin gene promoter. Recombinant HBsAg consisting of a polypeptide of molecular weight ∼36 kDa (p36) was purified by gel filtration and affinity chromatography to 92% purity. __________ Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 477–480, September–October, 2005.     

Induction of the PAOX1-Promoter and Synthesis of GFP Protein in Wild X-33 Strain Recombinant <Emphasis Type="Italic">Pichia pastoris</Emphasis> Yeast Cells

Recombinant clones of X-33 strain Pichia pastoris containing the marker gene yEGFP were prepared. The optimal methanol concentration in the medium for induction of heterologous expression was determined in the recombinant clones.__________Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 60–62, January–February, 2005.     

Chemical Composition and Antimicrobial Activity of Essential Oil from the Aerial Part of Haplophyllum latifolium

Chemistry of Natural Compounds -     

Interaction of abscisic-acid-binding cotton (Gossypium hirsutum) protein and phytohormones

The interaction of representatives of three classes of phytohormones, abscisic acid (ABA), the synthetic auxin 1-NAA, and the synthetic cytokinin 6-BAP, with ABA-binding cotton protein was studied. Binding of ABA with its protein was shown to be specific and receptor-like. Competitive protein binding showed that 1-NAA (15±3%), 6-BAP (82±3%), and ABA (95±3%) replace³H-ABA from the protein complex. The possibility of reacting ABA-binding protein with various classes of intracellular phytohormones is discussed.Institute of Genetics and Experimental Biology of Plants, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (99871) 64 22 30. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 252–253, May–June, 2000.     

Chemical composition,antimicrobial and antioxidant activities of the essential oils of three Uzbek Lamiaceae species

The chemical composition of the essential oil from the aerial parts of three Lamiaceae species from Uzbekistan was investigated by GC-MS analysis. β-Linalool (26.6%), α-terpineol (10.0%), coumarin (8.9%) and 4,5,7,7α-tetrahydro-4,4,7α-trimethyl-2(6H)-benzofuranone (5.4%) resulted as the main components of Ajuga turkestanica essential oil, while camphene (17.1%), 1,8-cineole (15.9%), β-cymene (7.9%) and limonene (7.4%) in Phlomis regelii. The essential oil of Thymus seravschanicus was dominated by thymol (37.5%), phellandral (26.0%), τ-terpinene (6.6%) and β-cymene (5.2%). The essential oils had considerable antimicrobial activity against different bacterial strains and fungi. Among the tested samples of essential oils, P. regelii essential oil has the significant antioxidant activity with IC₅₀ value of 117.8 ± 8.02 μg/mL.     

Synthesis of co-oligomers of 2-propenylphenol with maleic anhydride and study of products of their transformations with amines as steel corrosion inhibitors

Modification with diethylamine and diethanolamine of co-oligomers of 2-propenylphenol with maleic anhydride yielded water-soluble nitrogen-containing derivatives inhibiting the corrosion of St.3 steel in watersalt solutions.     

Immunochemical Study of Antigenic Determinants of Recombinant HBsAg Produced by Bombyx mori Larvae

Antibodies to various protein fractions isolated from larvae of mulberry silkworm containing recombinant HBsAg, to plasma HBV, and to healthy human serum were obtained. The cross reactivity of the antibodies was studied using enzyme-linked immunosorbent assay (ELISA). The antigenic determinants of recombinant HBsAg are identical to those in plasma and have practically no common antigenic determinants with healthy human blood serum     

Primary structure of triacetinase — An esterase from cotton seeds III. Peptides from tryptic hydrolysis

Summary 1. The peptides from tryptic hydrolysis of triacetinase have been isolated and characterized and their amino-acid sequences have been determined.2. On the basis of the results of the determination of the amino-acid sequences of the peptides of the tryptic hydrolyzate the sequence of peptides of the cyanogen bromide hydrolysis of triacetinase has been established or confirmed.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 241–248, March–April, 1978.