Effect of spermine and DNase on DNA release from bacteriophage T5

Bacterial viruses (bacteriophages) consist of nucleic acid protected by a protein envelope called capsid. At the start of infection, the phage genome is translocated into the bacterial cytoplasm. In vitro (and also in vivo), this DNA release can be triggered by binding a specific receptor protein to the phage tail. The force responsible for the release arises from energy stored in the capsid due to strong confinement of the DNA. We show that this force can be modified by adding molecules like spermine that affect DNA conformation. The tetravalent cation spermine can reduce the pressure inside the capsid and induce condensation of the released DNA. We examine the effect of spermine on DNA ejection from phage T5 by using light scattering and gel electrophoresis to measure the amount of DNA remaining in the capsid at the end of ejection. We discuss the results in terms of free energy minimization and we demonstrate that the presence of a DNA condensate outside the phage generates an additional force pulling passively on the DNA remaining inside the capsid.     

Studies on chargino production and decay at a photon collider

A Monte-Carlo analysis on production and decay of supersymmetric charginos at a future photon-collider is presented. A photon collider offers the possibility of a direct branching-ratio measurement. In this study, the process has been considered for a specific mSUGRA scenario. Various backgrounds and a parameterised detector simulation have been included. Depending on the centre-of-mass energy, a statistical error for the directly measurable branching ratio BR( ) of up to 3.5% can be reached.Received: 14 March 2005, Revised: 31 May 2005, Published online: 28 June 2005G. Klämke: Now at: Institut für Theoretische Physik, Universität Karlsruhe, Wolfgang-Gaede-Str. 1, 76131 Karlsruhe, Germany     

Impedance spectroscopy study of the sintering of yttria-stabilized zirconia/magnesia composites

In the present study, the sintering of (ZrO₂:8 mol%Y₂O₃)_1 ? m–(MgO)_m, YSZ–mMg composites, with m in the 0–30 mol% range, has been investigated by impedance spectroscopy (IS), dilatometry, and X-ray diffraction. Impedance diagrams were collected at 400 °C after heating the green compacts up to a selected sintering temperature, which was increased stepwise from 800 to 1400 °C. The combined experimental results revealed that the samples can be separated in two categories: below and above the solubility limit of MgO in the YSZ (m ~ 10). Moreover, important microstructural features associated with both the sintering process and solid solution formation of YSZ–mMgO samples were correlated to the electrical properties inferred by IS.     

The multiple-scale transport equation in one space dimension

This study examines the behavior of the one-dimensional non-homogeneous transport equation of the form u_t= u_x+f, «1. The solution consists of behavior which changes on two different time scales, one rapid and one slow. This time scale behavior is known. Additionally, however, we find here that because of the presence of the non-homogeneous forcing termf, and large wave speed 1/, there is a component of the solution which will vary only on a very large spatial scale. This large space-scale solution persists throughout all time, even after the source term of the solution has been shut off. The analysis of this large spacescale behavior is the focus of this paper. Numerical experiments highlight some of our results. These results have applications in fields such as meteorology, and other areas where multiple time scales are of interest.This work was supported in part by NSF grant NSF-DMS93-21728.     

Existence and fractional regularity of solutions for a doubly nonlinear differential inclusion

This article considers the issues of existence and regularity of solutions to the following doubly nonlinear differential inclusion $$\omega_t+\alpha (\omega_t)-\Delta \omega-\Delta_p{\omega} \ni f$$ where α is a maximal monotone operator in ${\mathbb{R}^2}$ and Δ _p denotes the p-Laplacian with p > 2. The investigation on fractional regularity is based on the Galerkin method combined with a suitable basis for W ^1,p , which we exhibit as a preliminary result. This approach also allows the obtaining of estimates in the so-called Nikolskii spaces, since it balances the interplay between the maximal monotone operator with the appearing higher order nonlinear terms.     

Magnetically induced chessboard pattern in the conductance of a Kondo quantum dot

We quantitatively describe the main features of the magnetically induced conductance modulation of a Kondo quantum dot-or chessboard pattern-in terms of a constant-interaction double quantum dot model. We show that the analogy with a double dot holds down to remarkably low magnetic fields. The analysis is extended by full 3D spin density functional calculations. Introducing an effective Kondo coupling parameter, the chessboard pattern is self-consistently computed as a function of magnetic field and electron number, which enables us to explain our experimental data quantitatively.     

Tunable coupling of superconducting qubits

We study an LC circuit implemented using a current-biased Josephson junction (CBJJ) as a tunable coupler for superconducting qubits. By modulating the bias current, the junction can be tuned in and out of resonance and entangled with the qubits coupled to it. One can thus implement two-qubit operations by mediating entanglement. We consider the examples of CBJJ and charge-phase qubits. A simple recoupling scheme leads to a generalization to arbitrary qubit designs.     

Experimental implementation of an adiabatic quantum optimization algorithm

We report the realization of a nuclear magnetic resonance computer with three quantum bits that simulates an adiabatic quantum optimization algorithm. Adiabatic quantum algorithms offer new insight into how quantum resources can be used to solve hard problems. This experiment uses a particularly well-suited three quantum bit molecule and was made possible by introducing a technique that encodes general instances of the given optimization problem into an easily applicable Hamiltonian. Our results indicate an optimal run time of the adiabatic algorithm that agrees well with the prediction of a simple decoherence model.     

On Multi-threaded Metrical Task Systems

Traditionally, on-line problems have been studied under the assumption that there is a unique sequence of requests that must be served. This approach is common to most general models of on-line computation, such as Metrical Task Systems. However, there exist on-line problems in which the requests are organized in more than one independent thread. In this more general framework, at every moment the first unserved request of each thread is available. Therefore, apart from deciding how to serve a request, at each stage it is necessary to decide which request to serve among several possibilities.In this paper we introduce Multi-threaded Metrical Task Systems, that is, the generalization of Metrical Task Systems to the case in which there are many threads of tasks. We study the problem from a competitive analysis point of view, proving lower and upper bounds on the competitiveness of on-line algorithms. We consider finite and infinite sequences of tasks, as well as deterministic and randomized algorithms. In this work we present the first steps towards a more general framework for on-line problems which is not restricted to a sequential flow of information.