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101.
The advent of chemical tools for cellular imaging—from organic dyes to green fluorescent proteins—has revolutionized the fields
of molecular biology and biochemistry. Lanthanide-based probes are a new player in this area, as the last decade has seen
the emergence of the first responsive luminescent lanthanide probes specifically intended for imaging cellular processes.
The potential of these probes is still undervalued by the scientific community. Indeed, this class of probes offers several
advantages over organic dyes and fluorescent proteins. Their very long luminescence lifetimes enable quantitative spatial
determination of the intracellular concentration of an analyte through time-gating measurements. Their emission bands are
very narrow and do not overlap, enabling the simultaneous use of multiple lanthanide probes to quantitatively detect several
analytes without cross-interference. Herein we describe the principles behind the development of this class of probes. Sensors
for a desired analyte can be designed by rationally manipulating the parameters that influence the luminescence of lanthanide
complexes. We will discuss sensors based on varying the number of inner-sphere water molecules, the distance separating the
antenna from the lanthanide ion, the energies of excited states of the antenna, and PeT switches.
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Valérie C. PierreEmail: |
102.
Jahjah M Belahsene S Nähle L Fischer M Koeth J Rouillard Y Vicet A 《Optics letters》2012,37(13):2502-2504
A system for gas sensing based on the quartz-enhanced photoacoustic spectroscopy technique has been developed. It makes use of a quantum well distributed feedback (DFB) laser diode emitting at 3.38 μm. This laser emits near room temperature in the continuous wave regime. A spectrophone, consisting of a quartz tuning fork and two steel microresonators were used. Second derivative wavelength modulation detection is used to perform low concentration measurements. The sensitivity and the linearity of the Quartz enhanced photoacoustic spectroscopy (QEPAS) sensor were studied. A normalized noise equivalent absorption coefficient of 4.06×10(-9) cm(-1)·W/Hz(1/2) was achieved. 相似文献