A New Isolate from Fusarium proliferatum (AUF-2) for Efficient Nitrilase Production

A fungal isolate from Fusarium proliferatum strain AUF-2 has been found to have a high nitrilase activity (≥1,000 U/l culture). The present work describes optimization of growth conditions and production medium to achieve maximum nitrilase production. The most effective carbon and nitrogen sources for nitrilase production were glucose and sodium nitrate, respectively. ε-Caprolactam was found to be the best inducer for maximum nitrilase production with 80 g/l wet cell biomass and 26 U/g nitrilase activity. An overall nitrilase activity of ≥2,000 U/l culture was obtained in this study, which is one of the best activities reported so far in any Fusarium strain. Chemo-profiling has shown that the strain is versatile in its ability to hydrolyze both aliphatic as well as aromatic nitriles. Efforts are being made to use the strain for biotransformation of pharmaceutical substrates.     

Synthesis,Characterization, and Thermal and Antibacterial Studies of Diorganotin(Iv) Derivatives of Amino Acids

Abstract

Six diorganotin(IV) derivatives of α-aminoacids with general formulae [(CH₃)₂ SnAACl]₂ and [(CH₃CH₂CH₂CH₂)₂SnAACl]₂, where AA = L-alaninate, L-phenylalaninate, and L-isoleucinate, have been synthesized by reacting dimethyltin(IV) dichloride (M) and dibutyltin(IV) dichloride (B) with L-alanine (A) or L-phenylalanine (PA) or L-isoleucine (I) using acetonitrile as the solvent and designated as MA, MPA, MI, BA, BPA, and BI. These complexes have been characterized by elemental analysis, infrared (IR), ¹H NMR, ¹³C NMR, and ¹¹⁹Sn NMR spectroscopy. Thermal studies of all of the synthesized complexes were also carried out using thermogravimetric (TG) and differential scanning calorimetry (DSC) techniques. The thermal decomposition mechanisms were similar for MA, BA, MI, and BI and occurred in one step, while in compounds MPA and BPA, it occurred in two consecutive steps. The TG curves of MPA and BPA suggest the loss of the ligand (AA) in the first step, with probable formation of a tin oxide R₂SnO as an intermediate, and in the second step, free tin is obtained, similar to MA, BA, MI, and BI, in accordance with the stoichiometry of the related derivatives. The diorganotin(IV) complexes have also been screened for their antibacterial activity against Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa. The minimum inhibitory concentration values of these complexes show enhanced activity.     

Synthesis and Antimicrobial Screening of Some Novel 2‐(5‐(4‐(1H‐Benzo[d][1,2,3]triazol‐1‐yl)phenyl)‐4,5‐dihydro‐1H‐pyrazol‐3‐yl)phenols Incorporated by Triazole Moiety

A novel series of 2‐(5‐(4‐(1H‐benzo[d][1,2,3]triazol‐1‐yl)phenyl)‐4,5‐dihydro‐1H‐pyrazol‐3‐yl)phenols derivative has been synthesized from (E)‐3‐(4‐(1H‐benzo[d][1,2,3]triazol‐1‐yl)phenyl)‐1‐(2‐hydroxyphenyl)prop‐2‐en‐1‐ones in ethanol and hydrazine hydrate under reflux condition. The synthesized compounds were screened for antibacterial activity against Gram‐positive bacteria viz Staphylococcus aureus and Bacillus subtilis and Gram‐negative bacteria viz Escherichia coli and Salmonella typhi, respectively. Some of the tested compounds showed significant antimicrobial activity. IR, ¹H NMR, mass spectral data, and elemental analysis elucidated the structures of all the newly synthesized compounds.     

An experimental and theoretical reinvestigation of the Michael addition of primary and secondary amines to dimethyl acetylenedicarboxylate

Theoretical calculations of the Michael addition of diethylamine, pyrrolidine, and benzylamine to DMAD at the DFT (B3LYP/6-31+G^∗) level indicate that the reaction follows a stepwise mechanism via a zwitterionic intermediate. The reactions have low activation barriers, 13–15 kcal mol⁻¹ and are exothermic, ΔH° = −29 to −44 kcal mol⁻¹. The detailed investigation of the reaction of benzylamine with DMAD reveals participation of the reactant-, transition structure-, and the product-complexes and that the 1,3-prototropic shift occurs through the benzylamine molecule. It also predicts formation of dimethyl 2-(N-benzylimino)butane-1,4-dicarboxylate as one of the products, which has been duly isolated and characterized experimentally.     

Differential dynamical effects of macromolecular crowding on an intrinsically disordered protein and a globular protein: implications for in-cell NMR spectroscopy

In-cell NMR provides a valuable means to assess how macromolecules, with concentrations up to 300 g/L in the cytoplasm, affect the structure and dynamics of proteins at atomic resolution. Here an intrinsically disordered protein, alpha-synuclein (alphaSN), and a globular protein, chymotrypsin inhibitor 2 (CI2) were examined by using in-cell NMR. High-resolution in-cell spectra of alphaSN can be obtained, but CI2 leaks from the cell and the remaining intracellular CI2 is not detectable. Even after stabilizing the cells from leakage by using alginate encapsulation, no CI2 signal is detected. From in vitro studies we conclude that this difference in detectability is the result of the differential dynamical response of disordered and ordered proteins to the changes of motion caused by the increased viscosity in cells.     

Randers change of <Emphasis Type="Italic">m</Emphasis><Superscript>th</Superscript> root metric

The present paper deals with a Randers metric that has been derived after a particular β-change in the mth root metric. Various geometers such as [7], [9], [10] etc. have studied the mth root metric and its transformations. We have obtained some tensors and theorems holding the relation between the Finsler space equipped with the mth root metric and the one obtained after its Randers change.     

Synthesis of a 1,3,5-benzotriazepine-2,4-dione based library

A library of benzotriazepines have been synthesised employing microwave-mediated synthesis, supported resins and parallel synthesis methodology.     

Fluorescence Quenching of Tris(2,2′-bipyridine)Ruthenium(II) Dichloride by Certain Organic Dyes

Fluorescence quenching of [Ru(bpy)₃]²⁺ by a series of organic dyes has been investigated by using the steady state fluorescence technique in aqueous medium. The dyes used are anthraquinone dyes: uniblue, acid blue 129, alizarin, alizarin red S and the azo dyes: congo red, sunset yellow, methyl orange, tartrazine, acid orange 63, methyl red and erichrome black T. The quenching of [Ru(bpy)₃]²⁺ was found to obey the Stern-Volmer equation and the corresponding Stern-Volmer plots were linear indicating dynamic quenching. The quenching rate constants (k _q) were calculated from the fluorescence data. The mechanism of quenching was discussed on the basis of the quenching rate constants as well as the reduction potential of dyes. The electron transfer mechanism has been proved by the calculation of Gibbs energy changes (ΔG _et) by applying the Rehm-Weller equation.     

Ruthenium(III) catalyzed oxidation of paracetamol by chloramine-T in aqueous alkaline medium – A kinetic and mechanistic pathway

The kinetic study of ruthenium(III) chloride catalyzed oxidation of paracetamol by N-chloro-p-toluene sulfonamide (chloramine-T) in the alkaline medium has been performed. The reaction exhibits second order nature and the effect of the catalyst indicates the occurrence of uncatalyzed reaction simultaneously. Rate is decelerated by hydroxide ions. A plausible reaction mechanism has been suggested and the rate law is derived to account for such experiential observations. The activation parameters have been calculated. No evidence of the participation of free radicals is observed.