miR-9 and let-7g enhance the sensitivity to ionizing radiation by suppression of NFκB1

The activation of nuclear factor-kappa B1 (NFkB1) in cancer cells may confer resistance to ionizing radiation (IR). To enhance the therapeutic efficiency of IR in lung cancer, we screened for microRNAs (miRNAs) that suppress NFkB1 and observed their effects on radiosensitivity in a human lung cancer cell line. From time series data of miRNA expression in γ-irradiated H1299 human lung cancer cells, we found that the expression of miR-9 was inversely correlated with that of NFκB1. Overexpression of miR-9 down-regulated the level of NFκB1 in H1299 cells, and the surviving fraction of γ-irradiated cells was decreased. Interestingly, let-7g also suppressed the expression of NFκB1, although there was no canonical target site for let-7g in the NFκB1 3' untranslated region. From these results, we conclude that the expression of miR-9 and let-7g could enhance the efficiency of radiotherapy for lung cancer treatment through the inhibition of NFκB1.     

Synthesis of polyfunctional triethoxysilanes by ‘click silylation’

The copper-catalyzed ‘click silylation’ has been exploited for the chemical modification of γ-azidopropyltriethoxysilane (AzPTES) with a wide range of terminal alkynes (1a–1v) in a one-pot operation. The novel 1,2,3-triazole-triethoxysilane derivatives (2a–2v) were synthesized by this procedure and comprehensively characterized by IR spectra, ¹H and ¹³C NMR, and HRMS studies.     

Study of Optical Properties of <Emphasis Type="Italic">Macrophomina phaseolina</Emphasis> Impregnated Sol-gel Derived Silica Matrices

In the present frame of work, Macrophomina phaseolina is encapsulated in silica matrices at various concentrations by low temperature sol-gel technique using tetraethylorthosilicate (TEOS) as precursor. The optical and photophysical properties of these samples have been studied by second harmonics of Nd:YAG laser at 532 nm. UV-visible absorption spectra of samples have been recorded and it is found that the absorption increases with increase in concentration of fungus. Further, a decrease in output transmission intensity of the laser has been observed with increase in fungus concentration. The temporal response of these samples has also been examined. The results show that the fungus concentration can be measured within ∼15–20 min. This method of optical sensing of fungus in test sample is faster than other techniques, such as the conventional colorimetric method which takes about 1 h.     

Rate constants of O2(1Δg)

The rate constants of O₂(¹Δ_g) with aliphatic alcohols, terpenes, unsaturated hydrocarbons, chlorinated hydrocarbons, oxygen, and diamines have been studied in thepresence of NO₂. The rate constants for oxygen, 1,2-ethane diamine, and 1,2-propane diamine are (9.9 ± 0.4) × 10², (8.7 ± 0.7) × 10⁴, and (1.4 ± 0.3) × 10⁴ 1/mol/s, respectively. The rate constants for all other compounds are less than the oxygen rate constant.