Immobilization of protein on ferromagnetic Dacron

Ferromagnetic Dacron (polyethyleneterephthalate) is proposed as a matrix to immobilize proteins covalently. Dacron in powder was magnetized by reacting ferrous (Fe+2) and ferric (Fe+3) ions with its hydrazide form at pH 8.3. Ferromagnetic hydrazide Dacron was converted to ferromagnetic azide Dacron and amyloglucosidase (E.C. 3.2.1.3) was covalently bound through the latter group. The catalytic property of the enzyme was preserved (8% of the specific activity estimated for the soluble enzyme) and all the magnetic amyloglucosidase Dacron derivative was recovered by using a magnetic field. No activity was detected in the supernatant.     

New mono- and bis-carbene samarium complexes: synthesis, X-ray crystal structures and reactivity

New samarium carbene complexes have been synthesized and characterized by X-ray diffraction analysis; the carbenic nature was assessed by reactivity studies.     

Quaternarisation de l'atome d'azote de systemes tautomeres de la serie des oxo-4 thiazoles. Cycloadditions dipolaires 1,3 des thiazoles mesoioniques formes in situ

4-Hydroxythiazoles and N-acylimino 4-thiazolidinones show similar tautomeric behaviour to azlactones. However, the mechanism of their reaction with dimethylacetylenedicarboxylate or with ethyl vinyl ether differs fundamentally. In the thiazole series, the first step is the quaternization of the nitrogen atom. The mesoionic thiazole intermediate is then trapped by a second molecule of the dipolarophile.     

19F NMR chemical shifts of n-F-alkyl compounds

The examination of ¹⁹F chemical shifts for ca. 650 F-alkylated compounds of general formula CF₃(CF₂)_nCF₂X led to the following conclusions: the CF₂ groups α to X are very sensitive to the nature of X, and are spread over a range of 85 ppm. The effect of the length of the F-alkyl chain decreases rapidly, so that δCF₂(α) can already be considered as characteristic of X for n = 1 or 2 for most practical purposes. Solvent effects (in 9 different solvents having ε = 1.8 to 52.1) were found to be rather small except for the F-alkyl iodides. A chart which indicates the domain in which the CF₂(α) resonance signal is to be expected is given for 42 different series of F-alkylated compounds; it is expected to provide the synthetic chemist with a useful tool for the identification and characterization of such compounds.     

Selective Determination of Nanogram γ-Globulin in Human Blood Serum by Resonance Light Scattering of Functionalized Nano-PbS

Functionalized nano-PbS has been prepared and characterized. The functionalized nanoparticles have good dispersibility in water. Reaction of functionalized nano-PbS with γ-globulin (γ-IgG) results an enhanced resonance light scattering (RLS) around 385nm.However, when the content of HSA is lower than 0.5μg/ml^-1 the RLS enhancement is very weak and is nonlinear to concentration of HSA. Based on these results, a new direct quantitative determination method for γ-globulin in blood serum samples without separation is established.Under optimal conditions, the enhanced RLS intensity is in proportion to the γ-IgG concentration in the range 10-500ng/mL. The limit of detection is 2.75ng/mL. This method is proved to be very sensitive, rapid, simple and selective for detection of γ-IgG in blood serum.     

Structure and thermal expansion of LiGe2(PO4)3

LiGe₂(PO₄)₃ belongs to the Nasicon-type family. Room-temperature structure has been determined on a single crystal from 3D X-ray data. Thermal evolution of this structure has been established from neutron powder diffraction data between 300 and 1000 K using the Rietveld method. The thermal expansion is positive along the c-axis, whereas shrinking is observed along the a-axis below 900 K, followed by dilatation. The atomic displacements noted with increasing temperature are consistent with a model proposed for NaZr₂(PO₄)₃.     

Mechanism for resonant O electron stimulated desorption from condensed O2. Non-adiabatic transitions and dynamics

Absolute kinetic energy distributions and yields associated with ground state ³P and excited state ¹D oxygen atoms have been obtained for O⁻ anion electron stimulated desorption from condensed O₂ in the electron energy range 6–15 eV. The observed yields are understood as resulting essentially from dissociative electron attachment reactions via the two lowest ²Σ⁺_g O⁻₂ resonance states through adiabatic and non-adiabatic transitions to the limits O⁻(²P) + O(³P) and O⁻(²P) + O(¹D). The kinetic energy distributions show the prominent role of electron multiple collision processes and post-dissociation interactions of the O⁻ anions in the condensed phase.     

东太平洋深海锰结核的化学元素研究

用ICP-AES法测定了东太平洋深海锰结核样品中二十多种主，次及微量元素，结果表明，表面瘤状和表面光滑两种类型锰结核的化学元素含量有较明显的区别，如表面光滑的锰结核TiO2含较高，而表面瘤状的锰结核Mn/Fe之值较高等。     

Surface-enhanced Raman spectra study of metal complexes of N-D-glucosamine beta-naphthaldehyde and glycine and their interaction with DNA

Copper(II), zinc(II), cobalt(II) and cobalt(III) complexes of N-D-glucosamine beta-naphthaldehyde (C17H19O6N, NG) and glycine were synthesized. The four novel metal complexes, Cu(II)C19H28O11N2(CuGNG), Zn(II)C19H24O9N2 (ZnGNG), Co(II)C19H28O11N2(Co(II)GNG) and Co(III)C21H29O12N2(Co(III)GNG) were characterized by means of infrared (IR), electronic absorption spectroscopy and NMR etc. The surface-enhanced Raman spectra of the four complexes and their interaction with DNA were studied. By comparison of the surface-enhanced Raman spectra (SERS), the information of the four complexes' SER active sites and adsorption orientation were obtained. Combined with fluorescence spectra of Ethidium bromide (EthBr) DNA system, we concluded that none of the four complexes intercalate into DNA and that the presence of the glycine ligand lowered the anticancer activity of NG series complexes.