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91.
Sarah K?hler Tim Liebert Thomas Heinze Antje Vollmer Petra Mischnick Eugen M?llmann Winfried Becker 《Cellulose (London, England)》2010,17(2):437-448
Water-soluble hydroxyalkyl cellulose with a molar degree of substitution of up to 2.79 was prepared under completely homogeneous
reaction conditions in various ionic liquids without addition of inorganic bases. In acetate containing solvents the IL acts
as a catalyst. The substitution patterns of the cellulose ethers were analyzed by 13C NMR spectroscopy, 1H NMR spectroscopy after peracetylation and GLC/MS after permethylation and depolymerization. A diminished tendency towards
the formation of side chains compared to heterogeneously prepared hydroxyalkyl celluloses in the presence of inorganic bases
was found. 相似文献
92.
Falk Liebner Emmerich Haimer Martin Wendland Marie‐Alexandra Neouze Kerstin Schlufter Peter Miethe Thomas Heinze Antje Potthast Thomas Rosenau 《Macromolecular bioscience》2010,10(4):349-352
Bacterial cellulose produced by the gram‐negative bacterium Gluconacetobacter xylinum was found to be an excellent native starting material for preparing shaped ultra‐lightweight cellulose aerogels. The procedure comprises thorough washing and sterilization of the aquogel, quantitative solvent exchange and subsequent drying with supercritical carbon dioxide at 40 °C and 100 bar. The average density of the obtained dry cellulose aerogels is only about 8 mg · cm?3 which is comparable to the most lightweight silica aerogels and distinctly lower than all values for cellulosic aerogels obtained from plant cellulose so far. SEM, ESEM and nitrogen adsorption experiments at 77 K reveal an open‐porous network structure that consists of a comparatively high percentage of large mesopores and smaller macropores.
93.
Frank Dettner Anne Hänchen Dominique Schols Prof. Dr. Luigi Toti Dr. Antje Nußer Roderich D. Süssmuth Prof. Dr. 《Angewandte Chemie (International ed. in English)》2009,48(10):1856-1861
An adaptable approach : The first highly convergent stereoselective synthesis of feglymycin (see structure) and its enantiomer is based on the coupling of repeating peptide fragments. The use of weakly basic conditions throughout the synthesis suppressed the epimerization of sensitive aryl glycine units. Feglymycin has strong anti‐HIV activity as well as potent (previously identified as weak) antibacterial activity against Staphylococcus aureus.
94.
Antje Meltzer Carsten Präsang Dr. Carsten Milsmann Matthias Driess Prof. Dr. 《Angewandte Chemie (International ed. in English)》2009,48(17):3170-3173
The right mix does the trick : Elusive {Ni0(η6‐arene)} moieties can be dramatically stabilized by the N‐heterocyclic silylene ligand 1 , which has a zwitterionic mesomeric structure. The σ, π‐acid–base synergism between nickel and 1 explains the unexpectedly high stability of the new silylene complexes 2 , which enables arene exchange studies at a Ni0 center. Addition of B(C6F5)3 to 2 affords the zwitterionic silylene complex 3 (see scheme, R=2,6‐iPr2C6H3).
95.
Bunyakul N Edwards KA Promptmas C Baeumner AJ 《Analytical and bioanalytical chemistry》2009,393(1):177-186
Fluorescence and electrochemical microfluidic biosensors were developed for the detection of cholera toxin subunit B (CTB)
as a model analyte. The microfluidic devices were made from polydimethylsiloxane (PDMS) using soft lithography from silicon
templates. The polymer channels were sealed with a glass plate and packaged in a polymethylmethacrylate housing that provided
leakproof sealing and a connection to a syringe pump. In the electrochemical format, an interdigitated ultramicroelectrode
array (IDUA) was patterned onto the glass slide using photolithography, gold evaporation and lift-off processes. For CTB recognition,
CTB-specific antibodies were immobilized onto superparamagnetic beads and ganglioside GM1 was incorporated into liposomes. The fluorescence dye sulforhodamine B (SRB) and the electroactive compounds potassium hexacyanoferrate
(II)/hexacyanoferrate (III) were used as detection markers that were encapsulated inside the liposomes for the fluorescence
and electrochemical detection formats, respectively. Initial optimization experiments were carried out by applying the superparamagnetic
beads in microtiter plate assays and SRB liposomes before they were transferred to the microfluidic systems. The limits of
detection (LoD) of both assay formats for CTB were found to be 6.6 and 1.0 ng mL−1 for the fluorescence and electrochemical formats, respectively. Changing the detection system was very easy, requiring only
the synthesis of different marker-encapsulating liposomes, as well as the exchange of the detection unit. It was found that,
in addition to a lower LoD, the electrochemical format assay showed advantages over the fluorescence format in terms of flexibility
and reliability of signal recording. 相似文献
96.
[reaction: see text] A novel, biomimetic concept for the direct reductive amination of ketones is described that relies on selective imine activation by hydrogen bond formation. The mild, acid- and metal-free process requires only catalytic amounts of thiourea as hydrogen bond donor and utilizes the Hantzsch ester for transfer hydrogenation. The method allows the efficient synthesis of structurally diverse amines. 相似文献
97.
Pure tone gap stimuli with identical (within-channel) or dissimilar (between-channel) marker frequencies of 1 and 2 kHz were presented to young and old listeners in a two-interval forced choice gap detection task. To estimate the influence of extraneous duration cues on gap detection, thresholds in the between-channel conditions were obtained for two different sets of reference stimuli: reference stimuli that were matched to the overall duration of the gap stimulus, i.e., two markers plus the gap, and reference stimuli that were fixed at the combined duration of the two markers excluding the gap. Results from within-channel conditions were consistent with previous studies, i.e., there were small but highly reliable age differences, smaller gap thresholds at longer marker durations, and an interaction between the two variables. In between-channel conditions, however, age differences were not as clear cut. Rather, the effect of age varied as a function of duration cue and was more pronounced when stimuli were matched for overall duration than when the duration of the reference tone was fixed. 相似文献
98.
A microfluidic device capable of recirculating nano to microlitre volumes in order to efficiently mix solutions is described. The device consists of molded polydimethyl siloxane (PDMS) channels with pressure inlet and outlet holes sealed by a glass lid. Recirculation is accomplished by a repeatedly reciprocated flow over an iterated sawtooth structure. The sawtooth structure serves to change the fluid velocity of individual streamlines differently depending on whether the fluid is flowing backwards or forward over the structure. Thus, individual streamlines can be accelerated or decelerated relative to the other streamlines to allow sections of the fluid to interact that would normally be linearly separated. Low Reynolds numbers imply that the process is reversible, neglecting diffusion. Computer simulations were carried out using FLUENT. Subsequently, fluorescent indicators were employed to experimentally verify these numerical simulations of the recirculation principal. Finally, mixing of a carboxyfluorescein labeled DMSO plug with an unlabeled DMSO plug across an immiscible hydrocarbon plug was investigated. At cycling rates of 1 Hz across five sawtooth units, the time was recorded to reach steady state in the channels, i.e. until both DMSO plugs had the same fluorescence intensity. In the case of the sawtooth structures, steady state was reached five times faster than in channels without sawtooth structures, which verified what would be expected based on numerical simulations. The microfluidic mixer is unique due to its versatility with respect to scaling, its potential to also mix solutions containing small particles such as beads and cells, and its ease of fabrication and use. 相似文献
99.
100.
Antje Klockow H. Michael Widmer Renato Amado Aran Paulus 《Fresenius' Journal of Analytical Chemistry》1994,350(7-9):415-425
The detection limits of the ANTS (8-aminonaphthalene-1,3,6-trisulfonic acid) label and ANTS maltose as a model carbohydrate conjugate were investigated with on-column UV and laser induced fluorescence detection. Under capillary electrophoresis conditions, the concentration and mass detection limits were found to be 5×10–7 mol/l or 8 femtomole with UV and 5×10–8 mol/l or 400 attomole with laser induced fluorescence detection, respectively. Including the derivatization reaction, the best concentration detection limit increases to 1×10–6 mol/l carbohydrate. A model calculation shows that these detection levels are still insufficient to match those of current protein sequencing protocols.Derivatization conditions for dextran and polygalacturonic acid ladders are described with subsequent fast separation in a capillary electrophoresis system under acidic pH buffer conditions. Up to 30 oligomers could be separated in less than 10 min. The application of ANTS labelled carbohydrate analysis in the food industry is demonstrated with the carbohydrate fraction of sweets and the kinetic monitoring of the hydrolysis of polygalacturonic acid.The described ANTS derivitization protocol works with as little as 5 g carbohydrate as demonstrated with a complex oligosaccharide labelled in a reaction volume as little as 2 l. To demonstrate the applicability of this approach to complex carbohydrate analysis, an oligosaccharide mixture derived from human Immunoglobuline G was labelled and separated within 5 min. Separation efficiency and speed are superior to state-of-the-art chromatographic methods. Both electrophoretic and chromatographic methods are complementary because of their different separation mechanism. The implications of using capillary electrophoresis with laser induced fluorescence and appropriate labelling strategies for structural and compositional analysis of complex carbohydrates are discussed.Dedicated to Professor Dr. Dieter Klockow on the occasion of his 60th birthday 相似文献