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11.
Pasquale Laise Duccio Fanelli Annarosa Arcangeli 《Communications in Nonlinear Science & Numerical Simulation》2012,17(4):1795-1804
Apoptosis is a biological process crucial for the development and maintenance of healthy living organism. A deregulated apoptosis underlies many diseases, including cancer. Under hypoxic conditions, p53 starts to accumulate and competes with HIF-1 for their common binding target p300. This can lead to the repression of HIF-1, and trigger the apoptotic derive. In addition apoptosis is accompanied by an enhancement of potassium (K+) fluxes, which in turn create a low-potassium intracellular micro-environment, which cooperates to the activation of caspases, the final actors of the apoptotic cascade. Based on this scenario, we elaborate a dynamical model aimed at resolving the complex dynamical interplay between the aforementioned processes. In the ideal continuum limit, the model reduces to a system of coupled differential equations, whose dynamics is analytically inspected. 相似文献
12.
R. Gaudiuso M. Dell’Aglio O. De Pascale S. Loperfido A. Mangone A. De Giacomo 《Analytica chimica acta》2014
A modified version of the calibration-free (CF) method was applied to the analysis of a set of archaeological brooches made of various copper-based alloys and coming from the archaeological site of Egnatia (Apulia, Southern Italy). The developed methodology consists in determining the plasma temperature by reversing the set of equations employed in the usual CF algorithm, and it is thus referred to as “inverse method”. The plasma temperature is determined for one certified standard, by using its known elemental composition as an input data, and then applied to the set of unknown samples to evaluate their composition in a CF mode. The feasibility of such an approach is demonstrated by comparing the results obtained with classical LIBS (drawing calibration lines with a series of matrix-matched certified standards) and with independent measurements performed with a conventional technique (LA-ICP-MS). 相似文献
13.
Alessandro Bitetto Annarosa Mangone Rosa Maria Mininni Lorena C. Giannossa 《Journal of Chemometrics》2016,30(7):405-415
Statistical techniques, when applied to data obtained by chemical investigations on ancient artworks, are usually expected to recognize groups of objects to classify the archeological finds, to attribute the provenance of items compared with earlier investigated ones, or to determine whether an archaelogical attribution is possible or not. The statistical technique most frequently used in archeometry is the principal component analysis (PCA), because of its simplicity in theory and implementation. However, the application of PCA to archeometric data showed severe limitations because of its linear feature. Indeed, PCA is inadequate to classify data whose behavior describe a curve or a curved subspace of the original data space. As a consequence of it, an amount of information is lost because the multi‐dimensional data space is compressed into a lower‐dimensional subspace including principal components. The aim of this work is then to test a novel statistical technique for archeometry. We propose a nonlinear PCA method to extract maximum chemical information by plotting data on the smallest number of principal components and to answer archeological questions. The higher accuracy and effectiveness of nonlinear PCA approach with respect to standard PCA for the analysis of archeometric data are shown through the study of Apulian red figured pottery (fifth–fourth century BC) coming from some of the most relevant archeological sites of ancient Apulia (Monte Sannace (Gioia del Colle), Egnatia (Fasano), Canosa, Altamura, Conversano, and Arpi(Foggia)). Copyright © 2016 John Wiley & Sons, Ltd. 相似文献
14.
Resatorvid‐based Pharmacological Antagonism of Cutaneous TLR4 Blocks UV‐induced NF‐κB and AP‐1 Signaling in Keratinocytes and Mouse Skin 下载免费PDF全文
Jaroslav Janda Nichole B. Burkett Karen Blohm‐Mangone Vivian Huang Clara Curiel‐Lewandrowski David S. Alberts Emanuel F. Petricoin III Valerie S. Calvert Janine Einspahr Zigang Dong Ann M. Bode Georg T. Wondrak Sally E. Dickinson 《Photochemistry and photobiology》2016,92(6):816-825
Cutaneous exposure to solar ultraviolet (UV) radiation is a major causative factor in skin carcinogenesis, and improved molecular strategies for efficacious chemoprevention of nonmelanoma skin cancer (NMSC) are urgently needed. Toll‐like receptor 4 (TLR4) signaling has been shown to drive skin inflammation, photoimmunosuppression, and chemical carcinogenesis. Here we have examined the feasibility of genetic and pharmacological antagonism targeting cutaneous TLR4 for the suppression of UV‐induced NF‐κB and AP‐1 signaling in keratinocytes and mouse skin. Using immunohistochemical and proteomic microarray analysis of human skin, we demonstrate for the first time that a significant increase in expression of TLR4 occurs in keratinocytes during the progression from normal skin to actinic keratosis, also detectible during further progression to squamous cell carcinoma. Next, we demonstrate that siRNA‐based genetic TLR4 inhibition blocks UV‐induced stress signaling in cultured keratinocytes. Importantly, we observed that resatorvid (TAK‐242), a molecularly targeted clinical TLR4 antagonist, blocks UV‐induced NF‐κB and MAP kinase/AP‐1 activity and cytokine expression (Il‐6, Il‐8, and Il‐10) in cultured keratinocytes and in topically treated murine skin. Taken together, our data reveal that pharmacological TLR4 antagonism can suppress UV‐induced cutaneous signaling, and future experiments will explore the potential of TLR4‐directed strategies for prevention of NMSC. 相似文献
15.
Angela Traini Lorena C. Giannossa P. Ubbrìaco Annarosa Mangone Maria D. De Filippis R. Laviano 《Journal of Thermal Analysis and Calorimetry》2008,92(1):337-344
Two kilns, one of which collapsed during firing cycle together with its entire pottery load, have been excavated at the Egnazia
site in Southern Italy.
To understand the reason for the collapse, ‘Broad Line’ typology pottery finds were analysed by complementary analytical techniques.
Analytical results not only suggest as cause of collapse sudden overheating in kiln due to uncontrolled increases in temperature,
but also indicate a good technological cycle from the recovery of raw materials to the manufacturing and firing process, which
tends to disprove the common assumption of non-professional production. 相似文献
16.
Lorena Carla Giannossa Marianna Acquaviva Caterina Laganara Rocco Laviano Annarosa Mangone 《Applied Physics A: Materials Science & Processing》2014,116(4):1541-1552
Glazed pottery with “negative decoration” samples, dating back to the twelfth to thirteenth century ad and coming from three sites along the Adriatic coast, Siponto, Egnatia and Trani (Southern Italy) were characterized from physical–chemical, mineralogical and morphological points of view. Optical microscopy, scanning electron microscopy with energy dispersive X-ray spectroscopy, inductively coupled plasma-mass spectroscopy, X-ray diffraction and micro-Raman spectroscopy investigations were carried out on ceramic bodies, pigments and glazes of the fragments. We aimed to outline the technological features, define the nature of decorations and coatings—glazes and engobes—and look for clues to hypothesize provenance. Results obtained show clear differences in raw materials and production technology between the impressed ceramic of Islamic tradition and the incised one of Byzantine tradition. Regarding the latter, evidences of a non-local origin can be found in the compositional diversity of raw materials used for the ceramic bodies of fragments decorated with spiral and pseudo-kufic motifs, which stressed the use of clays so far not recorded in Apulia. At the same time, at least in the case of Siponto, the compositional similarity of both ceramic bodies and materials used under the glaze for impressed ceramic and painted polychrome ceramics (RMR and protomaiolica), more likely local production, could suggest that both were produced in the same workshops. 相似文献
17.
Lorena Carla Giannossa Daniela Fico Antonio Pennetta Annarosa Mangone Rocco Laviano Giuseppe Egidio De Benedetto 《Chemical Papers》2015,69(8):1033-1043
A multi-analytical approach was used to investigate Roman lead-glazed ceramic artefacts from archaeological excavations at Pompeii and Herculaneum (Italy) aiming at defining the production technology of both glaze and ceramic body, by way of integrated investigations. The chemical, structural, and micro-morphological characterisations were performed using a combination of laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS), optical microscopy (OM), scanning electron microscopy (SEM), and micro-Raman spectroscopy. Fragments of artefacts (skyphoi, oil lamps, bowls, askoi, amphorae, krateres) of great historical and archaeological interest were sampled. LA-ICP-MS was used to determine the elemental composition by virtue of its effective lateral resolution, its ability to detect most elements and also to analyse comparably small samples. All the archaeological objects were coated with a lead-based glaze produced using a lead oxide-plus-quartz mixture, with sodium/potassium feldspars added as a flux and two different metals used: copper and iron. Two types of ceramic pastes have been identified, but chemometric techniques support the hypothesis of a Campanian provenance for the raw materials. Degradation phenomena such as the partial devitrification of the glaze, i.e. the slow structural reorganisation towards stable crystalline phases, and the leaching by mineral dissolution in the soil, were determined. 相似文献
18.
Ditaranto N Loperfido S van der Werf I Mangone A Cioffi N Sabbatini L 《Analytical and bioanalytical chemistry》2011,399(1):473-481
Biological agents play an important role in the deterioration of cultural heritage causing aesthetic, biogeophysical and biogeochemical
damages. Conservation is based on the use of preventive and remedial methods. The former aims at inhibiting biological attack,
and the latter aims at eradicating the biological agents responsible for biodeterioration. Here, we propose the preparation
and the analytical characterisation of copper-based nanocoating, capable of acting both as a remedy and to prevent microbial
proliferation. Core–shell CuNPs are mixed with a silicon-based product, commonly used as a water-repellent/consolidant, to
obtain a combined bioactive system to be applied on stone substrates. The resulting coatings exert a marked biological activity
over a long period of time due to the continuous and controlled release of copper ions acting as biocides. To the best of
our knowledge, this is the first time that a multifunctional material is proposed, combining the antimicrobial properties
of nanostructured coatings with those of the formulations applied to the restoration of stone artworks. A complete characterisation
based on a multi-technique analytical approach is presented. 相似文献
19.
Fyrner T Lee HH Mangone A Ekblad T Pettitt ME Callow ME Callow JA Conlan SL Mutton R Clare AS Konradsson P Liedberg B Ederth T 《Langmuir : the ACS journal of surfaces and colloids》2011,27(24):15034-15047
We describe the synthesis of a series of mono-, di-, and trisaccharide-functionalized alkanethiols as well as the formation of fouling-resistant self-assembled monolayers (SAMs) from these. The SAMs were characterized using ellipsometry, wetting measurements, and infrared reflection-absorption spectroscopy (IRAS). We show that the structure of the carbohydrate moiety affects the packing density and that this also alters the alkane chain organization. Upon increasing the size of the sugar moieties (from mono- to di- and trisaccharides), the structural qualities of the monolayers deteriorated with increasing disorder, and for the trisaccharide, slow reorganization dynamics in response to changes in the environmental polarity were observed. The antifouling properties of these SAMs were investigated through protein adsorption experiments from buffer solutions as well as settlement (attachment) tests using two common marine fouling species, zoospores of the green macroalga Ulva linza and cypris larvae of the barnacle Balanus amphitrite. The SAMs showed overall good resistance to fouling by both the proteins and the tested marine organisms. To improve the packing density of the SAMs with bulky headgroups, we employed mixed SAMs where the saccharide-thiols are diluted with a filler molecule having a small 2-hydroxyethyl headgroup. This method also provides a means by which the steric availability of sugar moieties can be varied, which is of interest for specific interaction studies with surface-bound sugars. The results of the surface dilution study and the low nonspecific adsorption onto the SAMs both indicate the feasibility of this approach. 相似文献
20.
In this report we have studied the extraction of a series of heavy metals ions (Cu2+, Ni2+, Fe3+, Cr3+, CrO4(2-)) from water bulk solutions by means of reverse micelles. The parameters explored are the nature and concentration of the accompanying electrolyte, as well as the surfactant nature and its concentration. The extracted metals can be recovered and eventually concentrated in a new water solution carrying out a back extraction. The extracted amount of metal is strongly dependent on the charge of the metal to be extracted. Therefore the extracted water solution is enriched in higher charge metal. Anions of amphoteric metals, like the chromate ions, can be quantitatively separated from their positive cations, like Cr3+ by properly choosing the cationic or the anionic surfactants. The transfer of the metal is essentially controlled by electrostatic forces. A model based on the Poisson-Boltzmann distribution allow us to get the potential profile inside the water pool by determining the concentrations of the surfactant counter ions. From the potential profile and mass balance it is possible calculate the extraction percentage. 相似文献