A review of the chemical and physical properties of MnRe(CO)10 and its derivatives has been undertaken. This heterobimetallic complex is one of the simplest complexes known which contains a bond between two different metals and does not contain bridging groups. The review has revealed that high yield synthetic strategies are known for this complex and the synthetic strategies have bearing for the synthesis of other bimetallic complexes. Kinetic data suggest that information available on monometallic fragments can provide information on the reactivity trends expected for the dimer. However, the second metal is not a neutral spectator and provides a site forsecondary reactions eg for CO substitution. Data on the physical properties of the dimer are sparse and definitive statements ondMn-Re·vMn-Re, HMn-Re etc. cannot be made. This has significant implications for studies on related simple and complex dimer and cluster complexes. 相似文献
A novel approach for detection of ligand binding to a protein in solid samples is described. Hydrated precipitates of the anti-apoptotic protein Bcl-xL show well-resolved (13)C-(13)C 2D solid-state NMR spectra that allow site-specific assignment of resonances for many residues in uniformly (13)C-enriched samples. Binding of a small peptide or drug-like organic molecule leads to changes in the chemical shift of resonances from multiple residues in the protein that can be monitored to characterize binding. Differential chemical shifts can be used to distinguish between direct protein-ligand contacts and small conformational changes of the protein induced by ligand binding. The agreement with prior solution-state NMR results indicates that the binding pocket in solid and liquid samples is similar for this protein. Advantages of different labeling schemes involving selective (13)C enrichment of methyl groups of Ala, Val, Leu, and Ile (Cdelta1) for characterizing protein-ligand interactions are also discussed. It is demonstrated that high-resolution solid-state NMR spectroscopy on uniformly or extensively (13)C-enriched samples has the potential to screen proteins of moderate size ( approximately 20 kDa) for ligand binding as hydrated solids. The results presented here suggest the possibility of using solid-state NMR to study ligand binding in proteins not amenable to solution NMR. 相似文献
A broad understanding of the binding modes of ligands and inhibitors to cytochrome P450 is vital for the development of new drugs. We investigated ligand binding in a site-specific fashion on cytochrome P450 BM-3 from Bacillus megaterium, a 119 kDa paramagnetic enzyme, using solid-state magic angle spinning nuclear magnetic resonance methods. Selective labeling and longitudinal relaxation effects were utilized to identify the peaks in a site-specific fashion and to provide evidence for binding. Well-resolved one-dimensional and two-dimensional NMR spectra of cytochrome P450 BM-3 reveal shifts upon binding of its substrate, N-palmitoylglycine. These data are consistent with the crystallographic result that a biochemically important amino acid residue, Phe87, moves upon ligation. This experimental scheme provides a tool for probing ligand binding for complex systems. 相似文献
An ab initio study of O?N? N?S with full geometry optimization has been carried out to corroborate the presence of an interaction between the terminal atoms in this type of structure, which, in O?N? N?O, apparently stabilizes the cis conformer. Using the unscaled 4–31G basis set with a full set of d functions on the sulfur, there is a potential minimun at the trans but not the cis geometry. A gauche conformer with a torsional angle of 77.2° is the most stable. With N2O2 this basis set gives potential minima at both the cis and trans geometries, but the trans conformer is slightly more stable, contrary to experiment and the results of (7,3) basis-set calculations reported in the literature in which Gaussian lobe functions were employed. Using a (9,5) basis set there is no longer a potential minimum at the cis geometry, and a gauche structure is more stable than the cis conformer as in the case of N2OS with the less-extended basis set. Force constants (harmonic and anharmonic), compliance constants, relaxed force constants, and interaction-displacement coordinates for both molecules are compared for key structural elements. 相似文献
The structure of five conformations of the-lac inhibitor clavulanic acid have been optimized using ab initio gradient methods at the 4-21G level. The conformations of lowest energy possess an intramolecular H bond and also have the lowest pyramidization of N1. Different side-chain conformations lead to (i) differences in anomeric interactions and variations of the hydroxyethylidene geometry and (ii) changes in the geometry of the ring skeleton, which are smaller in the four- than in the five-membered ring.On leave from Universidad de Santiago de Compostela, Departamento de Química Física, Facultad de Química, Galicia (Spain). 相似文献
A routing R of a graph G is a set of n(n ? 1) elementary paths R(u, v) specified for all ordered pairs (u, v) of vertices of G. The vertex-forwarding index ξ(G) of G, is defined by Where ξ(G, R) is the maximum number of paths of the routing R passing through any vertex of G and the minimum is taken over all the routings of G. Let Gp denote the random graph on n vertices with edge probability p and let m = np. It is proved among other things that, under natural growth conditions on the function p = p(n), the ratio Tends to 1 in probability as n tends to infinity. 相似文献
Complexes of Dioxocyanogen (OCN)2 with TiCl4 and ZrCl4 A solution of (OCN)2 was obtained by reaction of AgOCN with Br2 in H2CCl2 at ?70°C. From this the complexes TiCl4(NCO)2 and ZrCl4(NCO)2 were obtained by addition of equimolar amounts of TiCl4 and ZrCl4, respectively. According to the i.r. and Raman spectra, the (NCO)2 ligand is bonded via its N atoms to the metal. 相似文献
A collaborative study was conducted to compare the VIDAS Salmonella (SLM) with Rappaport-Vassiliadis (RV) method for detection of Salmonella in foods to the current standard method presented in the U.S. Food and Drug Administration's Bacteriological Analytical Manual (BAM) and the culture method presented in AOAC's Official Methods of Analysis. The VIDAS SLM with RV method uses tetrathionate broth in combination with RV medium in place of selenite cystine broth for selective enrichment, thereby eliminating the hazardous waste issue for laboratories. Twenty five laboratories participated in the evaluation, each testing one or more of 8 test products: nonfat dry milk, dried egg, soy flour, lactic casein, milk chocolate, raw ground pork, raw ground turkey, and raw peeled shrimp. Results of the study showed no significant differences in the numbers of confirmed positive samples with the VIDAS SLM with RV procedure and the BAM/AOAC culture procedure. The VIDAS SLM with RV method was effective for rapid detection of Salmonella in foods. It is recommended that AOAC INTERNATIONAL modify the VIDAS Salmonella SLM procedure to include the RV method. 相似文献