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101.
The use of plasmid DNA in gene therapy and genetic vaccination has increased the need for scalable and sustainable production processes. One key challenge for bioprocess engineering is the separation of plasmid DNA from structurally related impurities. Affinity purification procedures allow a highly selective capturing of the target molecule. In this paper, we present the isolation of a his-tagged lac repressor, its non-covalent immobilisation to different matrices and binding of DNA, thus enabling us to screen for combinations of ligands and stationary phases by using a building block principle. 相似文献
102.
Giraud N Blackledge M Goldman M Böckmann A Lesage A Penin F Emsley L 《Journal of the American Chemical Society》2005,127(51):18190-18201
A detailed analysis of nitrogen-15 longitudinal relaxation times in microcrystalline proteins is presented. A theoretical model to quantitatively interpret relaxation times is developed in terms of motional amplitude and characteristic time scale. Different averaging schemes are examined in order to propose an analysis of relaxation curves that takes into account the specificity of MAS experiments. In particular, it is shown that magic angle spinning averages the relaxation rate experienced by a single spin over one rotor period, resulting in individual relaxation curves that are dependent on the orientation of their corresponding carousel with respect to the rotor axis. Powder averaging thus leads to a nonexponential behavior in the observed decay curves. We extract dynamic information from experimental decay curves, using a diffusion in a cone model. We apply this study to the analysis of spin-lattice relaxation rates of the microcrystalline protein Crh at two different fields and determine differential dynamic parameters for several residues in the protein. 相似文献
103.
104.
Nicole Bäuerle Anja Blatter Alfred Müller 《Mathematical Methods of Operations Research》2008,67(1):161-186
In this paper we investigate dependence properties and comparison results for multidimensional Lévy processes. In particular
we address the questions, whether or not dependence properties and orderings of the copulas of the distributions of a Lévy
process can be characterized by corresponding properties of the Lévy copula, a concept which has been introduced recently
in Cont and Tankov (Financial modelling with jump processes. Chapman & Hall/CRC, Boca Raton, 2004) and Kallsen and Tankov
(J Multivariate Anal 97:1551–1572, 2006). It turns out that association, positive orthant dependence and positive supermodular dependence of Lévy processes can be characterized in terms of the Lévy measure as well as in terms of the Lévy copula. As
far as comparisons of Lévy processes are concerned we consider the supermodular and the concordance order and characterize
them by orders of the Lévy measures and by orders of the Lévy copulas, respectively. An example is given that the Lévy copula
does not determine dependence concepts like multivariate total positivity of order 2 or conditionally increasing in sequence. Besides these general results we specialize our findings for subfamilies of Lévy processes. The last section contains some
applications in finance and insurance like comparison statements for ruin times, ruin probabilities and option prices which
extends the current literature.
Anja Blatter was supported by the Deutsche Forschungsgemeinschaft (DFG). 相似文献
105.
106.
107.
Adnan Zahirović Marina Cindrić Sandra Kraljević Pavelić Mirsada Hukić Anja Harej 《Journal of Coordination Chemistry》2017,70(24):4030-4053
Heteroleptic ruthenium(II) bioflavonoid complexes of quercetin, morin, chrysin, and 3-hydroxyflavone were prepared and their interaction with CT DNA and BSA along with antioxidant and in vitro anticancer and antimicrobial activities was investigated. The formulation and characterization of complexes were achieved through elemental and thermal analysis, mass spectrometry, 1H NMR spectroscopy along with infrared, electronic absorption, and emission spectroscopy as well as square-wave voltammetry, and magnetic and conductivity measurements. Ruthenium(II) is octahedrally coordinated in cationic complex species to two bidentate diimine ligands (2,2′-bipyridine or 1,10-phenanthroline) and one bidentate monobasic flavonoid ligand through 3,4-site of quercetin, morin, and 3-hydroxyflavone or 4,5-site of chrysin. Complexes bind CT DNA by intercalation and binding constants comparable to ethidium bromide or 10 times higher. Binding constants of complexes to BSA were several times higher compared to ibuprofen and diazepam, and suggest that the complexes have a strong affinity to BSA. Antioxidant activity tests showed that the complexes are more potent in terms of radical inhibition compared to the parent flavonoids. Cytotoxic testing revealed that the Ru(II) complex of quercetin with 2,2′-bipyridine co-ligand has good selectivity to breast adenocarcinoma, while the complex of 3-hydroxyflavone with 2,2′-bipyridine co-ligand showed strong cytotoxicity toward all tested cell lines with IC50 ~ 1 μM. All complexes showed moderate activity toward Acinetobacter baumannii, while the Ru(II) complex of 3-hydroxyflavone with 2,2′-bipyridine showed excellent activity toward MRSA and Candida albicans. 相似文献
108.
Kauffmann-Weiss S Gruner ME Backen A Schultz L Entel P Fähler S 《Physical review letters》2011,107(20):206105
We exploit the intrinsic structural instability of the Fe(70)Pd(30) magnetic shape memory alloy to obtain functional epitaxial films exhibiting a self-organized nanostructure. We demonstrate that coherent epitaxial straining by 54% is possible. The combination of thin film experiments and large-scale first-principles calculations enables us to establish a lattice relaxation mechanism, which is not expected for stable materials. We identify a low twin boundary energy compared to a high elastic energy as key prerequisite for the adaptive nanotwinning. Our approach is versatile as it allows to control both, nanostructure and intrinsic properties for ferromagnetic, ferroelastic, and ferroelectric materials. 相似文献
109.
Syntheses and Crystal Structures of Rb4Br2O and Rb6Br4O In the quasi‐binary system RbBr/Rb2O, the addition compounds Rb4Br2O and Rb6Br4O are obtained by solid state reaction of the boundary components RbBr and Rb2O. Crystals of red‐orange Rb4Br2O as well as of orange Rb6Br4O decompose immediately when exposed to air. Rb4Br2O (Pearson code tI14, I4/mmm, a = 544.4(6) pm, c = 1725(2) pm, Z = 2, 175 symmetry independent reflections with Io > 2σ(I), R1= 0.0618) crystallizes in the anti K2NiF4 structure type; Rb6Br4O (Pearson code hR22, R3c, a = 1307.8(3) pm, c = 1646.6(5) pm, Z = 6, 630 symmetry independent reflections with Io > 2σ(I), R1 = 0.0759) in the anti K4CdCl6 structure type. Both structures contain characteristic ORb6‐octahedra and can be understood as expanded perovskites, following the general systematics of alkaline metal oxide halides. 相似文献