Influence of the Degree of Deacetylation of Chitosan and BMP-2 Concentration on Biocompatibility and Osteogenic Properties of BMP-2/PLA Granule-Loaded Chitosan/β-Glycerophosphate Hydrogels

Compositions based on chitosan/β-glycerophosphate hydrogels with highly porous polylactide granules can be used to obtain moldable bone graft materials that have osteoinductive and osteoconductive properties. To eliminate the influence of such characteristics as chain length, degree of purification, and molecular weight on a designed material, the one-stock chitosan sample was reacetylated to degrees of deacetylation (DD%) of 19.5, 39, 49, 55, and 56. A study of the chitosan/β-glycerophosphate hydrogel with chitosan of a reduced DD% showed that a low degree of deacetylation increased the MSCs (multipotent stromal cells) viability rate in vitro and reduced the leukocyte infiltration in subcutaneous implantation to Wistar rats in vivo. The addition of 12 wt% polylactide granules resulted in optimal composite mechanical and moldable properties, and increased the modulus of elasticity of the hydrogel-based material by approximately 100 times. Excessive filling of the material with PLA (polylactide) granules (more than 20%) led to material destruction at a ~10% strain. Osteoinductive and osteoconductive properties of the chitosan hydrogel-based material with reacetylated chitosan (39 DD%) and highly porous polylactide granules impregnated with BMP-2 (bone morphogenetic protein-2) have been demonstrated in models of orthotopic and ectopic bone formation. When implanted into a critical-size calvarial defect in rats, the optimal concentration of BMP-2 was 10 μg/mL: bone tissue areas filled the entire material’s thickness. Implantation of the material with 50 μg/mL BMP-2 was accompanied with excessive growth of bone tissue and material displacement beyond the defect. Significant osteoinductive and osteoconductive properties of the material with 10 μg/mL of BMP-2 were also shown in subcutaneous implantation.     

The Influence of Silicon Dioxide on the Stability of the Phase Composition and Mechanical Properties of Alumina-Toughened Zirconia-Based Ceramics

Technical Physics - The influence of SiO2 impurity (with a concentration varying from 0 to 10 mol %) on the stability of tetragonal ZrO2 (t-ZrO2) and on a set of micro- and macromechanical...     

Vibration Properties of Thin Films of Transition 3d-Metals on the Cu (111) Surface

Russian Physics Journal - Using the potentials, generated by a tight binding approximation, the equilibrium atomic structure and the phonon spectra of Cr and Ni thin films (one monolayer) deposited...     

Gradient structure of polypropylene composites filled with carbon black

Russian Chemical Bulletin - Gradient polymer composites were prepared by melt-mixing of carbon black and polypropylene in a compounder and subsequent hot pressing of pellets. The thermally treated...     

Thermodynamic and structural characteristics of aqueous alkanolamine solutions

Journal of Structural Chemistry - Thermodynamic characteristics are calculated for aqueous alkanolamine solutions that are obtained by substituting alkyl radicals for the protons in the amino group...