Heat‐Free Biomimetic Metal Molding on Soft Substrates

Fabrication of bio‐templated metallic structures is limited by differences in properties, processing conditions, packing, and material state(s). Herein, by using undercooled metal particles, differences in modulus and processing temperatures can be overcome. Adoption of autonomous processes such as self‐filtration, capillary pressure, and evaporative concentration leads to enhanced packing, stabilization (jamming) and point sintering with phase change to create solid metal replicas of complex bio‐based features. Differentiation of subtle differences between cultivars of the rose flower with reproduction over large areas shows that this biomimetic metal patterning (BIOMAP) is a versatile method to replicate biological features either as positive or negative reliefs irrespective of the substrate. Using rose petal patterns, we illustrate the versatility of bio‐templated mapping with undercooled metal particles at ambient conditions, and with unprecedented efficiency for metal structures.     

Advances in ultra-high load polymer-supported peptide synthesis with phenolic supports: 2. Use of ‘hydrazinolysis-hplc’ to check for racemization on c-terminal amino acid loading and to determine peptide homogeneity during synthesis with selectively labile c-terminal anchoring linkages

2, 4-Dinitrophenyl-L-phenylalanine has been coupled to L-, D-, and DL-amino acid phenyl esters pendant upon a polymer matrix. The esters had been prepared by di-isopropylcarbodiimide-mediated condensation, catalyzed by 4-dimethylaminopyridine. Reverse phase high pressure liquid chromatography (HPLC), using elution solvents consisting of 10 vol.-% trifluoroacetic acid in water/acetonitrile mixtures, has been used to investigate the 2,4-dinitrophenyl L-L and L-D dipeptide mixtures obtained on hydrazinolysis of each of the dipeptide-matrix assemblies. ‘Hydrazinolysis-HPLC’ has been used also to determine intermediate peptide homogeneity in ultra-high load solid (gel) phase synthesis with Boc amino acids. Cross-linked poly(N-[2-(4-hydroxyphenyl)ethyl]acrylamide) and two derived polymers incorporating spacer groups have been used as supports. The spacer groups made possible peptide C-terminal attachment by either HF-labile benzyl ester or HF-labile cyclohexyl ester bonds, while still incorporating the phenolic ester linkage susceptible to rapid hydrazinolytic scission.     

High-field MRS studies in brain slices

We are applying multi-nuclear high-field (500 MHz) MR spectroscopy of metabolising whole tissue preparations of the mammalian brain to studies on individual components of convulsions, which include prolonged depolarization, metabolic deprivation, and the effects of excitotoxins. The responses of glial cells and neurones can be partially distinguished by following labelling patterns of metabolic intermediates from ¹³C-labelled glucose or acetate (which enters only glial cells). This approach clearly confirmed our earlier indications that the metabolic response to depolarization (40 mM extracellular K⁺) occurs essentially in glial cells. Some evidence for metabolic shuttling between glia and neurones was obtained from the changes in C3/C4 ratios of glutamate and glutamine, and the C2/C3 of GABA. Mechanisms for metabolic support of neurones by glia may be of importance in neuronal protection under such metabolic stress as occurs in epilepsy. Changes in free intracellular divalent cations ([Ca²⁺]_i and [Zn²⁺]_i) were monitored using the ¹⁹F-MRS indicator, 5FBAPTA. Large increases in [Ca²⁺]_i and decreases in PCr were produced by excitotoxins (glutamate and NMDA), depolarization or ischaemia, but intracellular Zn²⁺ appeared only after exposure to the excitotoxins. The NMDA receptor blocker, MK801, removed all of the responses to NMDA, but only prevented the appearance of Zn²⁺ observed with glutamate. These results indicate that the damage caused to neurones by such insults as convulsions is not due simply to the presence of excessive excitotoxic glutamate.     

NMR chemical shifts in amino acids: effects of environments in the condensed phase

We present calculations of NMR chemical shifts in crystalline phases of some representative amino acids such as glycine, alanine, and alanyl-alanine. We explore the effects of environment on the chemical shifts in selected glycine geometries ranging from the crystalline phase to completely isolated molecules. In the crystalline and dilute molecular limits, the calculated distinct NMR chemical shifts are attributed to intermolecular hydrogen-bonds and dipole electric field effects, respectively.     

Leggett-Garg inequality with a kicked quantum pump

A kicked quantum nondemolition measurement is introduced, where a qubit is weakly measured by pumping current. Measurement statistics are derived for weak measurements combined with single-qubit unitary operations. These results are applied to violate a generalization of the Leggett-Garg inequality. The violation is related to the failure of the noninvasive detector assumption, and may be interpreted as either intrinsic detector backaction, or the qubit entangling the microscopic detector excitations. The results are discussed in terms of a quantum point contact kicked by a pulse generator, measuring a double quantum dot.     

Hadron production at forward and backward rapidities in \sqrt {s_{NN} } = 200 GeV d+Au collisions

Understanding normal nuclear medium effects present in heavy ion collisions is essential for understanding the following dynamics of the high density matter produced in the collision. Asymmetric collisions, such as deuteron + gold, provide a key tool for studying these effects since particles produced in the forward and backward directions may be subject to different phenomena. Particle production has been studied in d+Au collisions for various kinematic regions at the RHIC facility. PHENIX has measured charged hadron production as a function of pT for different centrality classes using the PHENIX muon spectrometers for d+Au collisions at $\sqrt {s_{NN} } = 200$ GeV. The PHENIX muon spectrometers have coverage in both forward and backward directions in the rapidity range 1.2 |η| < 2.4. The R _cp measurement, the ratio of central to peripheral production, is presented and discussed. Comparisons are also made with some relevant theoretical calculations.     

Spectral beam combining of 2 μm Tm fiber laser systems

Output beams from three independently frequency-stabilized thulium master-oscillator power-amplifier fiber laser systems were spectrally combined using a plane-ruled metal diffraction grating. Two laser channels were frequency-stabilized with guided mode resonance filters and the third was stabilized using a plane-ruled metal diffraction grating. The systems had output wavelengths between 1984 and 2015 nm, each with a spectral width of 100-450 pm and output powers between 40-120 W. The combined beam had powers up to 49 W and was 32% efficient with respect to the launched pump power.     

Phospholipid lung surfactant and nanoparticle surface toxicity: Lessons from diesel soots and silicate dusts

Because of their small size, the specific surface areas of nanoparticulate materials (NP), described as particles having at least one dimension smaller than 100 nm, can be large compared with micrometer-sized respirable particles. This high specific surface area or nanostructural surface properties may affect NP toxicity in comparison with micrometer-sized respirable particles of the same overall composition. Respirable particles depositing on the deep lung surfaces of the respiratory bronchioles or alveoli will contact pulmonary surfactants in the surface hypophase. Diesel exhaust ultrafine particles and respirable silicate micrometer-sized insoluble particles can adsorb components of that surfactant onto the particle surfaces, conditioning the particles surfaces and affecting their in vitro expression of cytotoxicity or genotoxicity. Those effects can be particle surface composition-specific. Effects of particle surface conditioning by a primary component of phospholipid pulmonary surfactant, diacyl phosphatidyl choline, are reviewed for in vitro expression of genotoxicity by diesel exhaust particles and of cytotoxicity by respirable quartz and aluminosilicate kaolin clay particles. Those effects suggest methods and cautions for assaying and interpreting NP properties and biological activities.     

Motion of a viscoelastic micellar fluid around a cylinder: flow and fracture

We present an experimental study of the motion of a viscoelastic micellar fluid around a moving cylinder, which ranges from fluidlike flow to solidlike tearing and fracture, depending on the cylinder radius and velocity. The observation of crack propagation driven by the cylinder indicates an extremely low tear strength, approximately equal to the steady state surface tension of the fluid. At the highest speeds a driven crack is observed in front of the cylinder, propagating with a fluctuating speed equal on average to the cylinder speed, here as low as 5% of the elastic wave speed.     

Quasi-Gaussian Bessel-beam superposition: application to the scattering of focused waves by spheres

A superposition of zero-order Bessel beams is examined that closely resembles an idealized paraxial Gaussian beam, provided the superposition is not tightly focused. Plots compare wavefield properties in the focal region and in the far field for different values of kw(0), the product of the wavenumber k, and the focal-spot-radius w(0). The superposition (which is an exact solution of the Helmholtz equation) has the important property that the scattering by an isotropic sphere can be calculated without any approximations for the commonly considered case of linear waves propagating in an inviscid fluid. The nth partial wave amplitude is similar to the case of plane-wave illumination except for a weighting factor that depends on incomplete gamma functions. An approximation for the weighting factor is also discussed based on a generalization of the Van de Hulst localization principle for a sphere of radius a at the focus of a Gaussian beam. Examples display differences between the directionality of the scattering with the plane wave case even though for the cases displayed, ka does not exceed 2 and w(0)∕a is not less than 2. Properties of tightly focused wavefields and the partial wave weighting factors are discussed.