排序方式: 共有75条查询结果,搜索用时 31 毫秒
61.
S Chanda Sarmishtha Bhattacharyya Tumpa Bhattacharjee SS Ghugre Swapan Kumar Basu S Muralithar RP Singh B Mukherjee RK Bhowmik SN Ray 《Pramana》2001,57(1):175-179
The high spin states in N=80 139Pr have been investigated by in-beam γ-spectroscopic techniques following the reaction 130Te (14N, 5n) reaction at E=75 MeV, using a gamma detector array, consisting of seven 23% compton-suppressed high purity germanium detectors and a multiplicity
ball of fourteen bismuth germanate elements. Based on γ-γ coincidence data, the level scheme of 139Pr has been considerably extended up to 7.2 MeV excitation. Tentative spin-parity assignments are done for the newly proposed
levels on the basis of the DCO ratios corresponding to strong gates and the available information from the earlier light ion
experiments. 相似文献
62.
63.
The semileptonic decay width of heavy baryons such as (Λ
b
→ Λcev) has been estimated in the framework of a nonrelativistic field theoretic quark model where four component quark field
operators along with a harmonic oscillator wave function are used to describe translationally invariant hadronic states. The
present estimation does not make an explicit use of heavy quark symmetry and has a reasonable agreement with the experimentally
measured decay width, polarisation ratio and form factors with the harmonic oscillator radii and quark momentum distribution
inside the hadron as free parameters. 相似文献
64.
Isotope Substitution of Promiscuous Alcohol Dehydrogenase Reveals the Origin of Substrate Preference in the Transition State 下载免费PDF全文
Dr. Enas M. Behiry Dr. J. Javier Ruiz‐Pernia Dr. Louis Luk Prof. Dr. Iñaki Tuñón Prof. Dr. Vicent Moliner Prof. Dr. Rudolf K. Allemann 《Angewandte Chemie (International ed. in English)》2018,57(12):3128-3131
The origin of substrate preference in promiscuous enzymes was investigated by enzyme isotope labelling of the alcohol dehydrogenase from Geobacillus stearothermophilus (BsADH). At physiological temperature, protein dynamic coupling to the reaction coordinate was insignificant. However, the extent of dynamic coupling was highly substrate‐dependent at lower temperatures. For benzyl alcohol, an enzyme isotope effect larger than unity was observed, whereas the enzyme isotope effect was close to unity for isopropanol. Frequency motion analysis on the transition states revealed that residues surrounding the active site undergo substantial displacement during catalysis for sterically bulky alcohols. BsADH prefers smaller substrates, which cause less protein friction along the reaction coordinate and reduced frequencies of dynamic recrossing. This hypothesis allows a prediction of the trend of enzyme isotope effects for a wide variety of substrates. 相似文献
65.
The excitation event in Fourier transform ion cyclotron resonance mass spectrometry is optimized in order to obtain more reliable relative signal intensities and enhance the ion-selection performance in multi-tandem mass spectrometric experiments. Standard trapped-ion cells suffer from the undesirable ejection of ions along the symmetry axis (z-axis) of the cell, which is oriented parallel to the magnetic field lines. This z-eiection effect is difficult to predict, and thus difficult to avoid, especially when complicated broad-band excitation schemes are applied to the ions. An improved trapped-ion cell design, referred to as the ‘Infinity Cell’, is discussed which eliminates z-ejection. The Infinity Cell concept is based on the finding that it is possible to model the electric excitation field of an infinitely long cell with a cell of finite dimensions. The virtual elimination of z-ejection effects is demonstrated in several suitable experiments by comparing the operation of the standard cell and the Infinity Cell. 相似文献
66.
Nicoll AJ Weston CJ Cureton C Ludwig C Dancea F Spencer N Smart OS Günther UL Allemann RK 《Organic & biomolecular chemistry》2005,3(24):4310-4315
A peptide NTH-18 was synthesized in which a N-terminal helix is stabilised by two crossed disulfide bonds to a C-terminal extension. The design was inspired by the structure of the neurotoxic peptide apamin, which has previously been used to stabilise helices in miniature enzymes. CD- and NMR-spectroscopy indicated that NTH-18 adopted a fold similar to that found in apamin. However, the arrangement of the elements of secondary structures was inverted relative to apamin; a N-terminal alpha-helix was connected by a reverse turn to a C-terminal extension of non-canonical secondary structure. NTH-18 displayed significant stability to heat and changes of pH. The high definition of the N-terminal end of the alpha-helix of NTH-18 should make this peptide a useful vehicle to stabilise alpha-helices in proteins with applications in protein engineering and molecular recognition. 相似文献
67.
(1S,2R,6R,7R)-4-Phenyl-3,10-dioxa-5-azatricyclo[5.2.1.02,6]dec-4-en-9-one ((+)- 5 ) obtained in 6 steps from the Diels-Alder adduct of furan to 1-cyanovinyl (1S)-camphanate ((+)- 3 ) was reduced to the corresponding endo-alcohol (?)- 6 the treatment of which with HBr/AcOH provided (?)-(3aS,4S,6R,7S,7aR)-4β-bromo-3aβ,4,5,6,7,7aβ-hexahydro-2-phenyl-1,3-benzoxazole-6β,7α-diyl diacetate ((?)- 17 ). Elimination of HBr with 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU) and acidic hydrolysis furnished (?)-(1R,2S,3R,4R)-4-aminocyclohex-5-ene-1,2,3-triol ( ? (?)-conduramine C1;(?)- 1 ). 相似文献
68.
In the present paper we have investigated the self-focusing behaviour of radially symmetrical rippled Gaussian laser beam
propagating in a plasma. Considering the nonlinearity to arise from relativistic phenomena and following the approach of Akhmanov
et al, which is based on the WKB and paraxial-ray approximation, the self-focusing behaviour has been investigated in some detail.
The effect of the position and width of the ripple on the self-focusing of laser beam has been studied for arbitrary large
magnitude of nonlinearity. Results indicate that the medium behaves as an oscillatory wave-guide. The self-focusing is found
to depend on the position parameter of ripple as well as on the beam width. Values of critical power has been calculated for
different values of the position parameter of ripple. Effects of axially and radially inhomogeneous plasma on self-focusing
behaviour have been investigated and presented here. 相似文献
69.
RK Choudhury 《Pramana》2001,57(2-3):585-600
Nuclear fission process involves large scale shape changes of the nucleus, while it evolves from a nearly spherical configuration
to two separated fission fragments. The dynamics of these shape changes in the nuclear many body system is governed by a strong
interplay of the collective and single particle degrees of freedom. With the availability of heavy ion accelerators, there
has been an impetus to study the nuclear dynamics through the investigations of nucleus-nucleus collisions involving fusion
and fission process. From the various investigations carried out in the past years, it is now well recognized that there is
large scale damping of collective modes in heavy ion induced fission reactions, which in other words implies that nuclear
motion is highly viscous. In recent years, there have been many experimental observations in heavy ion induced fission reactions
at medium bombarding energies, which suggest possible occurrence of various non-equilibrium modes of fission such as quasi-fission,
fast fission and pre-equilibrium fission, where some of the internal degrees of freedom of the nucleus is not fully equilibrated.
We have carried out extensive investigations on the fission fragment angular distributions at near barrier bombarding energies
using heavy fissile targets. The measured fragment anisotropies when compared with the standard saddle point model (SSPM)
calculations show that for projectile-target systems having zero or low ground state spins, the angular anisotropy exhibits
a peak-like behaviour at the sub barrier energies, which cannot be explained by the SSPM calculations. For projectiles or
targets with large ground state spins, the anomalous peaking gets washed out due to smearing of the K-distribution by the intrinsic entrance channel spins. Recently studies have been carried out on the spin distributions of
fission fragments through the gamma ray multiplicity measurements. The fission fragments acquire spin mainly from two sources:
(i) due to rigid rotation of the nascent fragments at scission and (ii) due to statistical excitation of the spin bearing
collective modes in the fissioning nucleus. One of the collective modes — the tilting mode depends on the K quantum number and is responsible for the emission angle dependence of fragment spin. In our studies, we have shown conclusively
that the collective statistical spin modes get strongly suppressed for high K values corresponding to large rotational frequencies along the fission axis. These results bring out the importance of the
dynamical effects in the heavy ion induced fusion-fission reactions. The present article will review the work carried out
on the above aspects in heavy ion fission reactions as well as on the fission time scales, and some of the recent studies
on the mass-energy correlations of fission fragments at near-barrier bombarding energies. 相似文献
70.
Nicoll AJ Miller DJ Fütterer K Ravelli R Allemann RK 《Journal of the American Chemical Society》2006,128(28):9187-9193
The design, synthesis, and characterization of a folded high-affinity metal-binding peptide is described. Based on the previously described folded peptide NTH-18, in which an alpha-helix was constrained through two disulfide bonds to a C-terminal extension of noncanonical secondary structure, a peptide (1) was designed to contain two histidine residues in positions 3 and 7. Air oxidation of 1 led to the formation of peptide 2, which contained two intramolecular disulfide bonds. The presence of the two histidines significantly destabilized the alpha-helical structure of 2 when compared to NTH-18. However, CD spectroscopy revealed that the addition of certain transition metal ions allowed the reformation of a stable alpha-helix. CD, NMR, and EPR spectroscopy as well as MALDI-TOF mass spectrometry indicated that 2 bound to Cu2+ to form a 1:1 complex via the imidazoles of the two histidine side chains. A glycine displacement assay revealed a dissociation constant for this complex of 5 nM at pH 8, which is the lowest reported value for a designed Cu2+-binding peptide. This peptide displayed more than 100-fold selectivity for Cu2+ over Zn2+, Ni2+, and Co2+. The 1.05 A crystal structure of the Cu(II)-complex of 2 revealed a square-pyramidal coordination geometry and confirmed that 2 bound to copper in an alpha-helical conformation via its two histidine side chains. The high affinity metal binding of peptide 2 demonstrates that metals can be used for the selective nucleation of alpha-helices. 相似文献