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111.
H. Nölle A. Horn J. Hüve B. Nölle J. Reinhardt J. Schmand H. J. Andrä E. W. Weber 《Zeitschrift für Physik D Atoms, Molecules and Clusters》1994,30(2):227-229
Cooling and optical pumping by a circularly polarized two mode laser is applied to a Na atomic beam in transverse geometry. The low velocity components of the beam are transversly cooled to the Doppler limit temperature of 240 µK and are simultaneously spin polarized for more than 90%. 相似文献
112.
H. Grosse-Ruyken J. Bosholm G. Reinhardt 《Isotopes in environmental and health studies》2013,49(3):124-125
Von Zehntelgrammengen Gd2O3 und Dy2O3 wurden durch Verteilungschromatographie mit Di-(2-äthylhexyl)-phosphorsäurebenachbarte Seltenerdverunreinigungen so weit abgetrennt, daß sie aktivierungsanalytisch nicht mehr nachweisbar waren. Das entspricht unter den gegebenen Aktivierungsbedingungen einem Restgehalt von < 10?10% Eu bzw. < 10?7% Tb. 相似文献
113.
114.
Peptide‐Templated Acyl Transfer: A Chemical Method for the Labeling of Membrane Proteins on Live Cells 下载免费PDF全文
Ulrike Reinhardt Jonathan Lotze Sarah Zernia Dr. Karin Mörl Prof. Dr. Annette G. Beck‐Sickinger Prof. Dr. Oliver Seitz 《Angewandte Chemie (International ed. in English)》2014,53(38):10237-10241
The development of a method is described for the chemical labeling of proteins which occurs with high target specificity, proceeds within seconds to minutes, and offers a free choice of the reporter group. The method relies upon the use of peptide templates, which align a thioester and an N‐terminal cysteinyl residue such that an acyl transfer reaction is facilitated at nanomolar concentrations. The protein of interest is N‐terminally tagged with a 22 aa long Cys‐E3 peptide (acceptor), which is capable of forming a coiled‐coil with a reporter‐armed K3 peptide (donor). This triggers the transfer of the reporter to the acceptor on the target protein. Because ligation of the two interacting peptides is avoided, the mass increase at the protein of interest is minimal. The method is exemplified by the rapid fluorescent labeling and fluorescence microscopic imaging of the human Y2 receptor on living cells. 相似文献
115.
Dr. Sebastian Götze Dr. Nahid Azzouz Dr. Yu‐Hsuan Tsai Prof. Dr. Uwe Groß Anika Reinhardt Dr. Chakkumkal Anish Prof. Dr. Peter H. Seeberger Dr. Daniel Varón Silva 《Angewandte Chemie (International ed. in English)》2014,53(50):13701-13705
Around 2 billion people worldwide are infected with the apicomplexan parasite Toxoplasma gondii which induces a variety of medical conditions. For example, primary infection during pregnancy can result in fetal death or mental retardation of the child. Diagnosis of acute infections in pregnant women is challenging but crucially important as the drugs used to treat T. gondii infections are potentially harmful to the unborn child. Better, faster, more reliable, and cheaper means of diagnosis by using defined antigens for accurate serological tests are highly desirable. Synthetic pathogen‐specific glycosylphosphatidylinositol (GPI) glycan antigens are diagnostic markers and have been used to distinguish between toxoplasmosis disease states using human sera. 相似文献
116.
We simulate the homogeneous nucleation of ice from supercooled liquid water at 220 K in the isobaric-isothermal ensemble using the MW monatomic water potential. Monte Carlo simulations using umbrella sampling are performed in order to determine the nucleation free energy barrier. We find the Gibbs energy profile to be relatively consistent with that predicted by classical nucleation theory; the free energy barrier to nucleation was determined to be ~18 k(B)T and the critical nucleus comprised ~85 ice particles. Growth from the supercooled liquid gives clusters that are predominantly cubic, whilst starting with a pre-formed subcritical nucleus of cubic or hexagonal ice results in the growth of predominantly that phase of ice only. 相似文献
117.
Christos Apostolidis Dr. Bernd Schimmelpfennig Dr. Nicola Magnani Dr. Patric Lindqvist‐Reis Dr. Olaf Walter Dr. Richard Sykora Dr. Alfred Morgenstern Dr. Eric Colineau Dr. Roberto Caciuffo Prof. Dr. Reinhardt Klenze Dr. Richard G. Haire Dr. Jean Rebizant Dr. Frank Bruchertseifer Dr. Thomas Fanghänel Prof. Dr. 《Angewandte Chemie (International ed. in English)》2010,49(36):6343-6347
118.
Herrwerth S Eck W Reinhardt S Grunze M 《Journal of the American Chemical Society》2003,125(31):9359-9366
Protein resistance of oligoether self-assembled monolayers (SAMs) on gold and silver surfaces has been investigated systematically to elucidate structural factors that determine whether a SAM will be able to resist protein adsorption. Oligo(ethylene glycol) (OEG)-, oligo(propylene glycol)-, and oligo(trimethylene glycol)-terminated alkanethiols with different chain lengths and alkyl termination were synthesized as monolayer constituents. The packing density and chemical composition of the SAMs were examined by XPS spectroscopy; the terminal hydrophilicity was characterized by contact angle measurements. IRRAS spectroscopy gave information about the chain conformation of specific monolayers; the amount of adsorbed protein as compared to alkanethiol monolayers was determined by ellipsometry. We found several factors that in combination or by themselves suppress the protein resistance of oligoether monolayers. Monolayers with a hydrophobic interior, such as those containing oligo(propylene glycol), show no protein resistance. The lateral compression of oligo(ethylene glycol) monolayers on silver generates more highly ordered monolayers and may cause decreased protein resistance, but does not necessarily lead to an all-trans chain conformation of the OEG moieties. Water contact angles higher than 70 degrees on gold or 65 degrees on silver reduce full protein resistance. We conclude that both internal and terminal hydrophilicity favor the protein resistance of an oligoether monolayer. It is suggested that the penetration of water molecules in the interior of the SAM is a necessary prerequisite for protein resistance. We discuss and summarize the various factors which are critical for the functionality of "inert" organic films. 相似文献
119.
Gerwen Lammers Günter Roth Mathias Heck Roland Zengerle G. Sandra Tjabringa Elly M. Versteeg Theo Hafmans Ronnie Wismans Dieter P. Reinhardt Eugene T. P. Verwiel Patrick L. J. M. Zeeuwen Joost Schalkwijk Roland Brock Willeke F. Daamen Toin H. van Kuppevelt 《Macromolecular bioscience》2012,12(5):675-691
A papillary‐structured collagen fibril membrane is created, mimicking the 3D‐architecture of the human papillary dermis. Primary human keratinocytes cultured to confluency on papillar‐structured films are compared to keratinocytes cultured on flat membranes. Microscopical evaluation reveals the presence of morphologically distinct cells at the base of the papillar structures that are not observed on flat membranes. Gene expression microarrays and RT‐qPCR indicate that these cells are in a more proliferative/migrational state, whereas cells on flat membranes have a more differentiated expression profile. Immunohistochemical stainings confirm these results. In conclusion, specific collagen architecture can direct keratinocyte behavior, and this may be used to further improve skin regeneration.
120.