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71.
Campanelli Anna Rita Domenicano Aldo Macchiagodena Marina Ramondo Fabio 《Structural chemistry》2011,22(5):1131-1141
Reference values of the structural substituent parameters, S
E and S
R, measuring the electronegativity and resonance effects, respectively, of functional groups (Campanelli et al. J Phys Chem
A 107:6429–6440, 2003) have been determined from the benzene ring geometries of 100 Ph–X species, including different conformations of the same
molecule. Geometries have been obtained by quantum chemical calculations at the HF/6-31G*, HF/6-311++G**, and B3LYP/6-311++G**
levels of theory. The substituent parameters from HF/6-311++G** calculations are in close agreement with those determined
at the HF/6-31G* level. Using the B3LYP density functional yields S
E and S
R values which—in general—correlate well with the corresponding HF values. Exceptions occur with some charged groups, and,
in the case of S
E, with a few dipolar groups having very high or low electronegativities. S
R values from B3LYP calculations are about 22% smaller than the corresponding HF values. The variations of the benzene ring
geometry caused by electronegativity, resonance, and steric effects are illustrated in some detail. 相似文献
72.
Lavergne A Zhu Y Pizzino A Molinier V Aubry JM 《Journal of colloid and interface science》2011,360(2):645-653
Two agro-based anionic surfactants containing an isosorbide moiety have been synthesized and their amphiphilic properties evaluated. Since isosorbide is now considered as an important platform chemical of the starch industry, these compounds could represent bio-sourced alternatives to the alkyl ether sulfates (notably lauryl ether sulfate, LES) that are based on petroleum-derived ethylene oxides. As isosorbide is an asymmetric diol, two isomers can be prepared (2-O-dodecyl isosorbide sulfate and 5-O-dodecyl isosorbide sulfate) that show significantly different aqueous properties as regards to their Krafft temperatures and critical micellar concentrations. 5-O-dodecyl isosorbide sulfate is the most soluble and the most efficient surfactant. It possesses a much lower critical micelle concentration (cmc) than sodium dodecyl sulfate, SDS, leading to comparable foaming properties with a three times lower concentration. Its behavior compares well with the one of pure diethoxylated dodecyl sulfate that has also been prepared and evaluated in this work. 相似文献
73.
Laura Belvisi Gianpaolo Bravi Carlo Scolastico Anna Vulpetti Aldo Salimbeni Roberto Todeschini 《Journal of computer-aided molecular design》1994,8(2):211-220
Summary A 3D QSAR methodology based on the combined use of conformational analysis and chemometrics was applied to perform a comparative analysis of the 3D conformational features of 13 nonpeptide angiotensin II receptor antagonists showing different levels of binding affinity. Conformational analysis by using a molecular mechanics MM2 method was carried out for each of these structures to obtain conformational minima. These minima were described by ten interatomic distances which define the relative spatial disposition of five significant atoms belonging to relevant functional groups present in all the 13 molecules. The structure-activity relationship between the interatomic distances and the biological activity was then assessed by using chemometric methods (cluster analysis, principal component analysis, classification methods). With our indirect approach based on the search for geometrical similarity it was possible, even though structural information on the receptor active site was lacking, to identify the 3D geometrical requirements for the binding affinity of nonpeptide angiotensin II receptor inhibitors. 相似文献
74.
Portable chemiluminescence multiplex biosensor for quantitative detection of three B19 DNA genotypes
Mara Mirasoli Francesca Bonvicini Luisa Stella Dolci Martina Zangheri Giorgio Gallinella Aldo Roda 《Analytical and bioanalytical chemistry》2013,405(2-3):1139-1143
A miniaturized multiplex biosensor exploiting a microfluidic oligonucleotide array and chemiluminescence (CL) lensless imaging detection has been developed for parvovirus B19 genotyping. The portable device consists of a reaction chip, comprising a glass slide arrayed with three B19 genotype-specific probes and coupled with a polydimethylsiloxane microfluidic layer, and a charge-coupled device camera modified for lensless CL imaging. Immobilized probes were used in DNA hybridization reactions with biotin-labeled targets, and then hybrids were measured by means of an avidin-horseradish peroxidase (HRP) conjugate and CL detection. All hybridization assay procedures have been optimized to be performed at room temperature through the microfluidic elements of the reaction chip, with sample and reagents delivery via capillary force exploiting adsorbent pads to drive fluids along the microchannels. The biosensor enabled multiplex detection of all B19 genotypes, with detectability down to 80 pmol?L?1 for all B19 genotype oligonucleotides and 650 pmol?L?1 for the amplified product of B19 genotype 1, which is comparable with that obtained in traditional PCR-ELISA formats and with notably shorter assay time (30 min vs. 2 h). The specificity of the assay has been evaluated by performing DNA–DNA hybridization reactions among sequences with different degrees of homology, and no cross hybridizations among B19 genotypes have been observed. The clinical applicability has been demonstrated by assaying amplified products obtained from B19 reference serum samples, with results completely consistent with the reference PCR-ELISA method. The next crucial step will be integration in the biosensor of a miniaturized PCR system for DNA amplification and for heat treatment of amplified products. Figure
A portable multiplex biosensor was developed for detection and genotyping of parvovirus B19 DNA, exploiting lensless CL imaging. The reaction chip is composed of a polydimethylsiloxane microfluidic layer coupled with a glass slide on which oligonucleotide probes specific for three different B19 genotypes are covalently immobilized in a 3?×?3 array. The reaction chip was used in hybridization reactions with biotin-labeled targets and then hybrids were then detected by means of an avidin-HRP conjugate, upon addition of a CL substrate for HRP 相似文献
75.
Mycoestrogen determination in cow milk: Magnetic solid‐phase extraction followed by liquid chromatography and tandem mass spectrometry analysis 下载免费PDF全文
Anna Laura Capriotti Chiara Cavaliere Patrizia Foglia Giorgia La Barbera Roberto Samperi Salvatore Ventura Aldo Laganà 《Journal of separation science》2016,39(24):4794-4804
Recently, magnetic solid‐phase extraction has gained interest because it presents various operational advantages over classical solid‐phase extraction. Furthermore, magnetic nanoparticles are easy to prepare, and various materials can be used in their synthesis. In the literature, there are only few studies on the determination of mycoestrogens in milk, although their carryover in milk has occurred. In this work, we wanted to develop the first (to the best of our knowledge) magnetic solid‐phase extraction protocol for six mycoestrogens from milk, followed by liquid chromatography and tandem mass spectrometry analysis. Magnetic graphitized carbon black was chosen as the adsorbent, as this carbonaceous material, which is very different from the most diffuse graphene and carbon nanotubes, had already shown selectivity towards estrogenic compounds in milk. The graphitized carbon black was decorated with Fe3O4, which was confirmed by the characterization analyses. A milk deproteinization step was avoided, using only a suitable dilution in phosphate buffer as sample pretreatment. The overall process efficiency ranged between 52 and 102%, whereas the matrix effect considered as signal suppression was below 33% for all the analytes even at the lowest spiking level. The obtained method limits of quantification were below those of other published methods that employ classical solid‐phase extraction protocols. 相似文献
76.
Cavaliere C Foglia P Guarino C Nazzari M Samperi R Laganà A 《Rapid communications in mass spectrometry : RCM》2007,21(4):550-556
A liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) method for measurement of aflatoxins B1, B2, G1, and G2 in maize is described. Aflatoxins (AFs) were extracted from 1 g samples by using tri-portions of acetonitrile/water (80:20, v/v) (10 + 7 + 7 mL), and 2/5 of the extract diluted to 500 mL by water was cleaned up with a 100 mg Carbograph-4 cartridge. After the addition of the internal standard AFM1, the final extract was analyzed by LC/ESI-MS/MS in positive ion mode using multiple reaction monitoring with a triple-quadrupole instrument. A C(18) column thermostatted at 45 degrees C with a mobile phase gradient of acetonitrile/water with 2 mmol/L ammonium formate was used. Although the matrix suppression effect was negligible, quantitation was achieved by an external calibration procedure using matrix-matched standard solutions to improve accuracy. Sample recoveries at four spiking levels ranged from 81 to 101% (relative standard deviation (RSD) =12%), and the method quantification limit ranged from 0.1 to 0.6 microg/kg. This method is sensitive, accurate and selective, and can thus be applied as a confirmatory procedure in establishing non-compliance with EU permitted maximum levels (2 microg/kg for aflatoxin B1 and 4 microg/kg for total AFs in corn products). This work also includes an application to samples obtained from retail markets and from experimental trial fields. Of the 48 samples, 15 were found to be contaminated with AFs and, of these, 5 did not comply with European Union legislation (AFB1 =2 microg/kg). 相似文献
77.
Dionigi C Stoliar P Porzio W Destri S Cavallini M Bilotti I Brillante A Biscarini F 《Langmuir : the ACS journal of surfaces and colloids》2007,23(4):2030-2036
We demonstrate field effect transistors based on organic semiconductor molecules dispersed in a self-organized polystyrene (PS) latex bead matrix. An aqueous colloidal composite made of PS and tetrahexylsexithiophene (H4T6) is deposited with a micropipet into the channel of a bottom-contact field effect transistor. The beads self-organize into a network whose characteristic distances are governed by their packing. The semiconductor molecules crystallize in the interstitial voids, leading to the growth of large interconnected domains. Depending on the bead size and the ratio between H4T6 and PS, the fraction of the different phases in the polymorph can be controlled. In the transistors where the H4T6 metastable "red phase" is the largest, the device response and the charge mobility are comparable to those of sexithienyl thin films grown by high-vacuum sublimation. 相似文献
78.
Bogialli S Coradazzi C Di Corcia A Lagana A Sergi M 《Journal of AOAC International》2007,90(3):864-871
A rapid, specific, and sensitive procedure for determining residues of 4 widely used tetracycline antibiotics and 3 of their 4-epimers in cheese is presented. The method is based on the matrix solid-phase dispersion (MSPD) technique followed by liquid chromatography/tandem mass spectrometry (LC/MS/MS). After dispersing samples of mozzarella, asiago, parmigiano, gruyere, emmenthal, and camembert on sand, target compounds were eluted from the MSPD column by passing through it 6 mL water heated at 70 degrees C. After acidification and filtration, 200 microL of the aqueous extract was directly injected into the LC column. For analyte identification and quantification, MS data acquisition was performed in the multireaction monitoring mode, selecting 2 precursor ion-to-product ion transitions for each target compound. Hot water appeared to be an efficient extractant, because absolute recoveries were no lower than 78%. Using demeclocycline as a surrogate analyte, recoveries of analyte added to the 6 types of cheeses at the 30 ng/g level were 96-117%, with relative standard deviation (RSD) not higher than 9%. Statistical analysis of the mean recovery data showed that the extraction efficiency was not dependent on the type of cheese analyzed. This result indicates that this method could be applied to other cheese types not considered here. At the lowest concentration considered, i.e., 10 ng/g, the accuracy of the method ranged between 90 and 107%, with RSDs not larger than 12%. Based on a signal-to-noise ratio of 10, limits of quantitation were estimated to be 1-2 ng/g. 相似文献
79.
Bacaloni A Cavaliere C Foglia P Nazzari M Samperi R Laganà A 《Rapid communications in mass spectrometry : RCM》2007,21(7):1123-1130
A liquid chromatography/electrospray ionization tandem mass spectrometric method for analyzing organophophorus flame retardants and plasticizers in drinking and environmental waters was developed. Five alkyl phosphates, three chlorinated alkyl phosphates, two aryl phosphate and triphenylphosphine oxide were selected for this study. These compounds were extracted from water samples by a hydrophilic polymeric solid-phase extraction cartridge. Accuracy and precision were evaluated analyzing 0.5 L of water samples spiked at concentrations of 10 and 100 ng/L for drinking water and at 300 and 1000 ng/L for river water. Except for trimethyl phosphate, analyte recoveries were better than 80%, and were not dependent on the type of aqueous matrix in which they were dissolved. At the spike levels considered, within-day precision was between 3 and 12% for tap water and between 4 and 14% for river water, and estimated method quantification limits ranged from 0.2 to 3.9 ng/L. A short survey conducted by analyzing some river water samples (River Tiber) ascertained the presence of ten organophosphorus compounds at concentration levels ranging from a few nanograms per liter to 323 ng/L for tris(2-butoxyethyl) phosphate. 相似文献
80.
Cavaliere C Foglia P Guarino C Nazzari M Samperi R Laganà A 《Analytica chimica acta》2007,596(1):141-148
A liquid chromatography-tandem mass spectrometric with electrospray ionization (LC/ESI-MS/MS) method for determining the four naturally occurring aflatoxins (AFs) B1, B2, G1, and G2 in olive oil is proposed. AFs were extracted from oil sample by means of matrix solid phase dispersion (MSPDE), utilizing C18 as dispersing material. No further purification step, such as lipid removal, was performed. Aflatoxin M1, the hepatic metabolite of AFB1, was employed as internal standard. Olive oil extract was analyzed by LC/ESI-MS/MS in positive ionization mode, with multireaction monitoring acquisition. Due to a signal suppression ranging between 4 and 23%, quantitation was performed by matrix-matched calibration curves. The regression line coefficients of determination were above 0.9991. Sample recoveries ranged from 92 to 107%, with relative standard deviations below 13% for spiking levels between 0.5 and 5 ng g−1; method quantification limits ranged between 0.04 and 0.12 ng g−1. The developed LC/ESI-MS/MS method, although not as sensitive as LC coupled to fluorescence detection, is rapid, selective, accurate and precise, thus it can be used as confirmatory assay. The MSPDE appears suitable for application to other oleaginous matrices and for multiresidue investigation. 相似文献