Salt Stress Proteins Identified by a Functional Approach in Yeast

Summary. We have performed functional genomics of salt stress by overexpression of gene libraries in yeast and selection for salt tolerance. Thirty halotolerance genes were isolated from yeast, Arabidopsis, and sugar beet. The results indicate that Na⁺ transport (uptake, efflux, and compartmentation), sulfate activation, RNA processing, and protein synthesis are crucial for salt tolerance.Received September 3, 2002; accepted September 16, 2002 Published online June 12, 2003     

Concerted aminolysis of diaryl carbonates: Kinetic sensitivity on the basicity of the nucleophile,nonleaving group,and nucleofuge

The kinetics of the reactions of 4‐methylphenyl, phenyl, and 4‐chlorophenyl 2,4,6‐trinitrophenyl carbonates ( 1 , 2 , and 3 , respectively) with a series of anilines and secondary alicyclic (SA) amines has been carried out spectrophotometrically in 44 wt% ethanol–water, at 25.0°C, ionic strength 0.2 M. The Brønsted plots (statistically corrected) for the reactions of carbonates 1 – 3 with anilines and SA amines were linear with slopes (β_N) in the range of 0.69–0.78 and 0.45–0.48, respectively, attributed to a concerted mechanism. The negative values found for the sensitivity of log k_N to the basicity of the nonleaving (β_nlg) and leaving (β_lg) groups are discussed. Anilines are more reactive than isobasic SA amines, probably because of the greater steric hindrance offered by the latter. © 2012 Wiley Periodicals, Inc. Int J Chem Kinet 44: 604–611, 2012     

A comparison of Bayesian,Hazard, and Mixed Logit model of bankruptcy prediction

Computational Management Science - The purpose of this study is to examine the impact of the choice of cut-off points, sampling procedures, and business cycles on the forecasting accuracy of...     

Direct classification of olive oils by using two types of ion mobility spectrometers

In this work, we explored the use of an Ion Mobility Spectrometry (IMS) device with an ultraviolet (UV) source, and of a Gas Chromatographic (GC) column coupled to an IM Spectrometer with a tritium source, for the discrimination of three grades of olive oil, namely: extra virgin olive oil (EVOO), olive oil (OO) and pomace olive oil (POO). The three types of oil were analyzed with both equipment combinations as coupled to a headspace system and the obtained ion mobility data were consecutively processed with various chemometric tools. The classification rate for an independent validation set was 86.1% (confidence interval at 95% [83.4%, 88.5%]) with an UV-IMS and 100% (confidence interval at 95% [87%, 100%]) using a GC-IMS system. The classification rate was improved by using a more suitable ionization source and a pre-separation step prior to the IM analysis.     

D-optimal designs and N-way techniques to determine sulfathiazole in milk by molecular fluorescence spectroscopy

The present work proposes an analytical procedure to determine sulfathiazole in milk by using molecular fluorescence spectroscopy. For this sulfonamide the European Union in Regulation 37/2010 has established a maximum residue limit in milk of 100 μg kg(-1). The study includes the effect of six factors on the recovery of sulfathiazole. The factors are: (i) The one related to the matrix depending on the heat treatment of the milk (UHT, pasteurized); (ii) Those related to the protein precipitation step, namely the ratio between the volume of trichloroacetic acid (TCA) and milk, centrifugation speed and temperature; (iii) Those affecting the derivatization reaction: derivatization time and volume of fluorescamine. To do this, two chemometric tools are used together: a D-optimal design for studying the effect of the factors on the recovery of sulfathiazole, considerably reducing the number of needed experiments; and the second-order property of the PARAFAC (Parallel Factor Analysis) decomposition that avoids the need of fitting a new calibration model each time that the experimental conditions change. It has been found that the type of milk, the TCA:milk ratio and the volume of fluorescamine have significant effect on the response. The rest of factors and interactions are not significant. The best recovery is obtained with UHT milk, 4:6 rate for TCA:milk volumes and 40 μL of fluorescamine. In UHT milk, the mean recovery (n=5) in the optimal conditions is 88.7% (RSD=12.4%). As some non-linear behaviour may occur when using fluorescence spectroscopy, the calibration model that relates the fluorescence spectra with the concentration is computed by a partial least squares regression and a multi-layer feed-forward neural network. In both cases, the proposed procedures have been validated according to Decision 2002/657/EC, concluding that the two are accurate although the calibration model built with the neural network has better figures of merit, the decision limit (CCα) for x(0)=100 μg L(-1) is 103.3 μg L(-1) and the detection capability (CCβ) is 106.5 μg L(-1), with the probabilities of false noncompliance (α) and false compliance (β) equal to 5%.     

Geneticallly enginereed trehalose accumulation improves cryopreservation tolerance of chrysanthemum (Dendranthema grandiflorum kitam.) shoot-tips

Shoot-tips isolated from two transgenic lines of chrysanthemum (Dendranthema grandiflorum Kitam.) var. Indianapolis in vitro plantlets with induced capacity to biosynthesize trehalose, and from a non-transformed line, were subjected to cryopreservation using a vitrification procedure. After dissection, apices were precultured on semi-solid MS medium with 0.3 M sucrose for 4 days, loaded in a 0.4 M sucrose + 2 M glycerol solution for 20-30 min and exposed to PVS2 or PVS3 vitrification solutions for 0, 20, 40 or 60 min at room temperature prior to rapid immersion in liquid nitrogen. The highest shoot regeneration after cryopreservation was obtained with exposure to either PVS solution for 40 min. Plant regeneration from cryopreserved shoot-tips ranged between 48 percent and 67 percent for transgenic lines and between 33 percent and 36 percent for non-transgenic lines. No polymorphic loci were detected in plantlets regenerated from cryopreserved and non-cryopreserved shoot-tips with RAPD techniques using eight primers that amplified 101 monomorphic loci.     

Monochromatic Paths and at Most 2-Coloured Arc Sets in Edge-Coloured Tournaments

We call the tournament T an m-coloured tournament if the arcs of T are coloured with m-colours. If v is a vertex of an m-coloured tournament T, we denote by ξ(v) the set of colours assigned to the arcs with v as an endpoint. In this paper is proved that if T is an m-coloured tournament with |ξ(v)|≤2 for each vertex v of T, and T satisfies at least one of the two following properties (1) m≠3 or (2) m=3 and T contains no C₃ (the directed cycle of length 3 whose arcs are coloured with three distinct colours). Then there is a vertex v of T such that for every other vertex x of T, there is a monochromatic directed path from x to v. Received: April, 2003     

Preparation of uniform rich cholesterol unilamellar nanovesicles using CO2-expanded solvents

Nanoscopic and uniform unilamellar vesicles, rich in cholesterol, have been prepared by a new procedure, named "DELOS-SUSP", which is based on the depressurization of a cholesterol solution in CO2-expanded acetone into an aqueous solution containing a surfactant. The CO2 is used here as a cosolvent medium, allowing the straightforward preparation of vesicular systems with controlled size distribution, uniform shape, and stability unachievable by conventional mixing technologies. The resulting nanoscopic vesicular systems dispersed in water were characterized using dynamic light scattering, zeta potential, turbidity, and cryogenic transmission electron microscopy. The influence of operational parameters of this new methodology on the physicochemical characteristics of the vesicular systems is also reported.     

Mosaic CuI−CuII−InIII 2D Perovskites: Pressure-Dependence of the Intervalence Charge Transfer and a Mechanochemical Alloying Method

The perovskite (BA)₄[Cu^II(Cu^IIn^III)_0.5]Cl₈ ( 1_BA ; BA⁺=butylammonium) allows us to study the high-pressure structural, optical, and transport properties of a mixed-valence 2D perovskite. Compressing 1_BA reduces the onset energy of Cu^I/II intervalence charge transfer from 1.2 eV at ambient pressure to 0.2 eV at 21 GPa. The electronic conductivity of 1_BA increases by 4 orders of magnitude upon compression to 20 GPa, when the activation energy for conduction decreases to 0.16 eV. In contrast, Cu^II perovskites achieve similar conductivity at ≈50 GPa. The solution-state synthesis of these perovskites is complicated, with more undesirable side products likely from the precursor mixtures containing three different metal ions. To circumvent this problem, we demonstrate an efficient mechanochemical synthesis to expand this family of halide perovskites with complex composition by simply pulverizing together powders of 2D Cu^II single perovskites and Cu^IIn^III double perovskites.     

Richardson’s Theorem in <Emphasis Type="Italic">H</Emphasis>-coloured Digraphs

Let H be a digraph possibly with loops and D a finite digraph without loops whose arcs are coloured with the vertices of H (D is an H-coloured digraph). The sets V(D) and A(D) will denote the sets of vertices and arcs of D respectively. A directed path W in D is an H-path if and only if the consecutive colors encountered on W form a directed walk in H. A set \(N\subseteq \hbox {V}(D)\) is an H-kernel if for every pair of different vertices in N there is no H-path between them, and for every vertex \(u\in \hbox {V}(D){\setminus }N\) there exists an H-path in D from u to N. Let D be an m-coloured digraph. The color-class digraph of D, denoted by \({\mathscr {C}}_C(D\)), is the digraph such that: the vertices of the color-class digraph are the colors represented in the arcs of D, and \((i,j) \in A({\mathscr {C}}_C(D\))) if and only if there exist two arcs namely (u, v) and (v, w) in D such that (u, v) has color i and (v, w) has color j. Let \(W=(v_0, \ldots , v_n\)) be a directed walk in \({\mathscr {C}}_C(D)\), with D an H-coloured digraph, and \(e_i = (v_{i},v_{i+1})\) for each \(i \in \{0, \ldots ,n-1\}\). Let \(I = \{i_1, \ldots , i_k\}\) a subset of \(\{0, \ldots , n-1\}\) such that for 0 \(\le s \le n-1\), \(e_s \in \hbox { A}(H^c)\) if and only if \(s \in I\) (where \(H^c\) is the complement of H), then we will say that k is the \(H^c\)-length of W. Since V(\({\mathscr {C}}_C(D)) \subseteq \hbox {V}(H)\), the main question is: What structural properties of \({\mathscr {C}}_C(D)\), with respect to H, imply that D has an H-kernel? In this paper we will prove the following: If \({\mathscr {C}}_C(D)\) does not have directed cycles of odd \(H^c\)-length, then D has an H-kernel. Finally we will prove Richardson’s theorem as a direct consequence of the previous result.