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11.
Hydroxylation of lysine, one of posttranslational modifications of proteins, generates 5‐hydroxylysine (Koh) and plays a crucial role in regulating protein functions in cellular activity. We have developed a chemical labeling method of Koh. The 1,2‐aminoalcohol moiety of Koh in synthetic peptide sequences was trapped by an alkyne‐containing benzimidate to form a 2‐oxazoline ring. An additional ammonia treatment process removed the undesirable amidine residue formed between benzimidate and lysine. During the ammonia treatment, the oxazoline residue formed at Koh mainly remained intact, and the ring opening to the amide form was observed for only part of oxazoline, indicating that the chemical labeling is amino acid selective. Azide‐substituted biotin or fluorescent dye was attached to the peptide through Huisgen cycloaddition at Koh and converted into an alkyne‐labeled oxazoline form. The Koh‐labeling assay could provide a platform to enhance proteomic research of lysine hydroxylation. 相似文献
12.
Ken MotokuraShigekazu Matsunaga Akimitsu MiyajiTatsuaki Yashima Toshihide Baba 《Tetrahedron letters》2011,52(50):6687-6692
Proton-exchanged montmorillonite showed catalytic activity for intermolecular allylsilylation, arylsilylation, and terminal silylation of alkynes with allylsilanes. The reaction selectivity greatly depended on the solvent used. Reactions proceeded with various terminal alkynes and allylsilanes in good to moderate yields. The reaction pathways involving cationic Si species on the montmorillonite surface were also investigated. 相似文献
13.
Okamoto A 《Chemical Society reviews》2011,40(12):5815-5828
An excitonic interaction caused by the H-aggregation of fluorescent dyes is a new type of useful photophysical process for fluorescence-controlled nucleic acid sensing. This critical review points out the recent advances in exciton-controlled hybridization-sensitive fluorescent oligonucleotide (ECHO) probes, which have a fluorescence-labeled nucleotide in which two molecules of thiazole orange or its derivatives are linked covalently. ECHO probes show absorption shift and emission switching depending on hybridization with the target nucleic acid. The hybridization-sensitive fluorescence emission of ECHO probes and the further modification of probes have made possible a variety of practical applications, such as multicolor RNA imaging in living cells and facile detection of gene polymorphism (144 references). 相似文献
14.
An improved synthetic method of palladium(II) dinuclear macrocyclic complexes have been described. Each of the two isomers of the complexes [Pd2LBr2] has a macrocyclic ligand L in which two 2,6-bis(diaminomethyl)phenyl units coordinate to the Pd(II) centers with N, C, N donor atoms. Substitution of the bromo ligands of one of the isomer of the complexes with acetonitrile ligands affords a new dinuclear complex. Catalytic activities of these complexes were studied for the Mizoroki-Heck type reactions of iodobenzene and styrene. High turnover number up to 30,000 was achieved using one of the isomer of the complexes. 相似文献
15.
Ikeda S Kubota T Wang DO Yanagisawa H Yuki M Okamoto A 《Organic & biomolecular chemistry》2011,9(19):6598-6603
Fluorescent oligonucleotides have been designed on which two different energy transfer processes were mounted together: excitonic interaction and FRET. The fluorescence emission of the oligonucleotides was controlled well by the two different energy transfer processes, in response to their hybridization to the complementary RNA both in vitro and in cells. 相似文献
16.
Maity P Tsunoyama H Yamauchi M Xie S Tsukuda T 《Journal of the American Chemical Society》2011,133(50):20123-20125
A new class of monolayer-protected Au clusters with Au-C covalent bonds (organogold clusters) was synthesized by ligating phenylacetylene (PhC≡CH) to PVP-stabilized Au clusters. Matrix-assisted laser desorption ionization mass spectrometry revealed for the first time a series of stable compositions of the organogold (Au:C(2)Ph) clusters. 相似文献
17.
We present a fitting method for obtaining a functional form of the refractive index profile of planar microlenses made by ion exchange techniques from total shearing interferometric measurements. Compared to the usual power series expansion fit, this method allows a reduction in the number of coefficients needed to characterize a lens. 相似文献
18.
H. Takahashi N. Mri Y. Nagata S. Nakamura T. Uchida J. Akimitsu Y. Tokura 《Physica C: Superconductivity and its Applications》1993,210(3-4):485-488
The superconducting transition temperature (Tc) of Ba0.62K0.38Bio3 (Tc=30 K) has been measured under high pressure up to 8 GPa. It is observed that Tc increases initially with pressure, as reported by Uwe et al., Shirber et al. and Huang et al., but decreases above 4 GPa. The Hall coefficient of Ba0.62K0.38BiO3 has been measured up to 1.2 GPa. The absolute value of the Hall coefficient decreases with pressure by 10% GPa, the value of which is almost the same as that obtained in most CuO-based high-temperature superconductors. 相似文献
19.
The critical behavior of the electron paramagnetic resonance line widths have been studied in the quasi two-dimensional antiferromagnet MnTiO3. Line widths diverge like ΔH∝(T?TN)?0.49, which is in good agreement with the theoretical value predicted by Kawasaki. 相似文献
20.
We have developed a new fluorescent DNA sensor containing two pyrene-labeled nucleobases, (Pet)G and (Py)C, and the fluorescence color was altered by the salt-induced B-Z DNA transition. 相似文献