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101.
102.
Cationic liposomes enhanced firefly bioluminescent assay of bacterial ATP in the presence of an ATP extractant. 总被引:1,自引:0,他引:1
Naohiro Nakata Akihiko Ishida Hirofumi Tani Tamio Kamidate 《Analytical sciences》2003,19(8):1183-1185
Cationic liposomes composed of two components, diethylaminoethyl-carbamoyl cholesterol and phosphatidylcholine, were applied to an enhancer for a firefly bioluminescent (BL) assay of bacterial ATP in the presence of an ATP extractant. Trichloroacetic acid (TCA), which inhibits the activity of luciferase, was used as an ATP extractant. Cationic liposomes enhanced the BL intensity as long as luciferase was active. The detection limits for cell numbers of Escherichia coli extracts in the presence of cationic liposomes and in water alone were 199 and 897 colony forming units ml(-1), respectively. The sensitivity for bacterial ATP in the presence of cationic liposomes was improved by a factor of 2.5 times compared to that in the presence of diethylaminoethyl-dextran. 相似文献
103.
Tosylation of the secondary side of β-cyclodextrin, which has previously been incorrectly reported, can be achieved by tosyl transfer from 3-nitrophenyl toluenesulfonate to the C-2 hydroxyl of the cyclodextrin. 相似文献
104.
A statistical theory is proposed for the calculation of the total capture cross section of a neutrino by a nucleus. Close references are made to the statistical theory of β-decay and electron capture developed by Yamada and his collaborators. As a test of the theory, we calculate the total cross section for the capture of a solar neutrino by a 37Cl nucleus. 相似文献
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Akihiko Tanioka Atsushi Oobayashi Yuji Kageyama Keizo Miyasaka Kinzo Ishikawa 《Journal of Polymer Science.Polymer Physics》1982,20(12):2197-2208
Effects of carbon filler on the sorption and diffusion of carbon dioxide in natural rubber and in styrene-butadiene rubber have been studied. Sorption isotherms conform to Henry's law in unfilled rubber and to Langmuir's law in carbon black. The isotherms in filled rubber exhibit a combination of the two sorption modes. The Henry's law solubility parameter kD increases with carbon filler content; the Langmuir saturation constant C′A initially is constant with filler level, but then decreases abruptly when carbon particles begin to aggregate. The diffusion coefficient decreases with increasing filler content, presumably owing to geometric effects and to polymer chain immobilization in the interfacial regions. 相似文献
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