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71.
We study Abelian strings in a fixed de Sitter background. We find that the gauge and Higgs fields extend smoothly across the cosmological horizon and that the string solutions have oscillating scalar fields outside the cosmological horizon for all currently accepted values of the cosmological constant. If the gauge to Higgs boson mass ratio is small enough, the gauge field function has a power-like behaviour, while it is oscillating outside the cosmological horizon if Higgs and gauge boson mass are comparable. Moreover, we observe that Abelian strings exist only up to a maximal value of the cosmological constant and that two branches of solutions exist that meet at this maximal value. We also construct radially excited solutions that only exist for non-vanishing values of the cosmological constant and are thus a novel feature as compared to flat space–time. Considering the effect of the de Sitter string on the space–time, we observe that the deficit angle increases with increasing cosmological constant. Lensed objects would thus be separated by a larger angle as compared to asymptotically flat space–time. 相似文献
72.
Candida kefyr DSM 70106 was cultivated in a medium containing inulin as a carbon source. About 92% of the inulinase was recovered directly
from the medium. Different concentration (Cf) and enrichment (Ef) factors were obtained, using the following methods: Cross-flow filtration (microfiltration and cell diafiltration were carried
out using a rotary filter; enzyme ultrafiltration and diafiltration were performed using a cassette module): Cf = 7.5 and Ef = 2.2; liquid-liquid extraction ofN-Benzyl-N-Dodecyl-N-bis[2-hydroxyethyl] ammonium chloride (BDBAC) reversed micelles: Cf = 2.5 and Ef = 2.7; and expanded-bed adsorption: Cf = 2.8 and Ef = 4.3. 相似文献
73.
74.
Thereza Christina Vessoni Penna Angela Faustino Jozala Thomas Rodolfo Gentille Adalberto Pessoa Jr. Olivia Cholewa 《Applied biochemistry and biotechnology》2006,129(1-3):334-346
Nisin, a bacteriocin produced during the exponential growth phase of Lactococcus lactis ATCC 11454, inhibits the growth of a broad range of Grampositive bacteria. Gram-negative bacteria can also be inhibited by nisin with EDTA. In this study, nisin production was assayed by the agar diffusion method using Lactobacillus sake ATCC 15521 and a recombinant Escherichia coli DH5-α expressing the recombinant green fluorescent protein as the nisin-susceptible test organisms. The titers of nisin expressed and released in culture media were quantified and expressed in arbitrary units (AU/mL of medium) and converted to standard nisin concentration (Nisaplin®, 25 mg of pure nisin with an activity of 1×106 AU/mL). The expression and release of nisin by L. lactis in skimmed milk (9.09% total solids) with Man Rugosa Shepeer-Bacto Lactobacilli broth (1∶1) was monitored in a 5 L New Brunswick fermentor. Combining EDTA with nisin increased the bactericidal effect of nisin on the bacteria examined. The presence of EDTA was necessary to inhibit E. coli growth with nisin. L. sake was shown to be a good indicator for the evaluation of nisin release in the culture media, including with the addition of EDTA. 相似文献
75.
Penna Thereza Christina Vessoni Chiarini Eb Machoshvili Irene Alexeevna Ishii Marina Pessoa Adalberto 《Applied biochemistry and biotechnology》2002,98(1-9):791-802
The recombinant green fluorescent protein (gfp
uv
) was expressed by Escherichia coli DH5-α cells transformed with the plasmid pGFPuv. The gfp
uv
was selectively permeabilized from the cells in buffer solution (25 mM Tris-HCl, pH 8.0), after freezing (−70°C for 15 h), by four freeze (−20°C)/thaw cycles interlaid by sonication. The average
content of released gfp
uv
(experiment 2) was 7.76, 34.58, 39.38, 12.90, and 5.38%, for the initial freezing (−70°C) and the first, second, third and
fourth freeze/thaw cycles, respectively. Superfusion on freezing was observed between −11°C and −14°C, after which it reached
−20°C at 0.83°C/min. 相似文献
76.
Hyaluronic acid (HA) has been implemented for chemo and photothermal therapy to target tumour cells overexpressing the CD44+ receptor. HA-targeting hybrid systems allows carbon nanomaterial (CNM) carriers to efficiently deliver anticancer drugs, such as doxorubicin and gemcitabine, to the tumour sites. Carbon nanotubes (CNTs), graphene, graphene oxide (GO), and graphene quantum dots (GQDs) are grouped for a detailed review of the novel nanocomposites for cancer therapy. Some CNMs proved to be more successful than others in terms of stability and effectiveness at removing relative tumour volume. While the literature has been focused primarily on the CNTs and GO, other CNMs such as carbon nano-onions (CNOs) proved quite promising for targeted drug delivery using HA. Near-infrared laser photoablation is also reviewed as a primary method of cancer therapy—it can be used alone or in conjunction with chemotherapy to achieve promising chemo-photothermal therapy protocols. This review aims to give a background into HA and why it is a successful cancer-targeting component of current CNM-based drug delivery systems. 相似文献
77.
Jos A Hernndez-Díaz Jorge JO Garza-García Janet M Len-Morales Adalberto Zamudio-Ojeda Jenny Arratia-Quijada Gilberto Velzquez-Jurez Julio C Lpez-Velzquez Soledad García-Morales 《Molecules (Basel, Switzerland)》2021,26(19)
The use of selenium nanoparticles (SeNPs) in the biomedical area has been increasing as an alternative to the growing bacterial resistance to antibiotics. In this research, SeNPs were synthesized by green synthesis using ascorbic acid (AsAc) as a reducing agent and methanolic extract of Calendula officinalis L. flowers as a stabilizer. Characterization of SeNPs was performed by UV-vis spectrophotometry, infrared spectrophotometry (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM) techniques. SeNPs of 40–60 nm and spherical morphologies were obtained. The antibacterial activity of marigold extracts and fractions was evaluated by disk diffusion methodology. The evaluation of SeNPs at different incubation times was performed through the colony-forming unit (CFU) count, in both cases against Serratia marcescens, Enterobacter cloacae, and Alcaligenes faecalis bacteria. Partial antibacterial activity was observed with methanolic extracts of marigold leaves and flowers and total inhibition with SeNPs from 2 h for S. marcescens, 1 h for E. cloacae, and 30 min for A. faecalis. In addition, SeNPs were found to exhibit antioxidant activity. The results indicate that SeNPs present a potentiated effect of both antimicrobial and antioxidant activity compared to the individual use of marigold extracts or sodium selenite (Na2SeO3). Their application emerges as an alternative for the control of clinical pathogens. 相似文献
78.
Flavia Barone Adalberto Bonincontro Filomena Mazzei Anna Minoprio Francesco Pedone 《Photochemistry and photobiology》1995,61(1):61-67
Abstract— It is well known that the pyrimidine dimers are the main damage produced by UV radiation on the DNA structure. However, while studies on the photoproduct structure have been carried out extensively, uncertainties still exist on the implication that a single damaging event has on the overall conformation. In particular, the extension of the damage influence on the polynucleotide chain is a matter of debate. This problem is especially important to understanding some steps of the repair mechanisms. In this study we performed a chemical-physical characterization of 21 base pair oligonucleotides containing a single thymine dimer in one strand. Thermodynamic parameters were determined by means of thermal denaturation experiments, and static fluorescence measurements were performed to unequivocally define the primary structure-conformation relationship in this specific case. We used hydroxyl radicals, produced by means of γ-irradiation of the sample solution, to detect fine structure changes. Our data show that the introduction of a single thymine dimer might cause only a slight distortion of the helix geometry, as judged by the evaluation of the enthalpic and the entropic terms and by the small changes observed in the binding of ethidium bromide to DNA. The modifications in the sugar phosphate backbone subsequent to the damaging event are especially evident, near the thymine dimer, toward the 5'-end direction in the strand containing the dimer. 相似文献
79.
Solatie D Carbol P Betti M Bocci F Hiernaut T Rondinella VV Cobos J 《Fresenius' Journal of Analytical Chemistry》2000,368(1):88-94
The choice of the analytical method for the determination of actinide isotopes in leachate solutions has to be made considering several parameters: detection limit for each isotope, sample preparation procedure in terms of duration and complexity, counting time and interferences. A leachate solution obtained by keeping a pellet of UO2 doped with 238Pu in contact with distilled water was investigated for the content of U and Pu isotopes by radiometric methods (alpha-, gamma-spectrometry and liquid scintillation counting). The results of the radiometric methods were compared with those obtained from the analysis performed by inductively coupled plasma mass spectrometry on-line to a system for chromatographic separation (IC-ICP-MS). The comparison confirmed that IC-ICP-MS is a powerful method for the detection of long-lived radionuclides. The radiometric methods have a detection limit two orders of magnitude lower than IC-ICP-MS in the case of short-lived radioisotopes mostly due to the low background in the detector. On the other hand, the sample preparation and the analysis duration are more time-consuming compared to IC-ICP-MS; moreover, not all isotopes can be determined by using only one radiometric technique. 相似文献
80.