Quorum sensing (QS) is the communication between bacterial cells governed by their population density and regulated by the genes controlling virulence factors and biofilm formation. Multiple mechanisms of biofilms are resistive to antimicrobial chemotherapy; therefore novel strategies are required to overcome its limitations. Here, we report the effect of various copper oxide nanostructures (CuO-NSs) on quorum sensing inhibition. The two-dimensional CuO-NSs such as interlaced nanodiscs, nanodiscs and leaf-shaped nanosheets are prepared via a simple chemical method. The Quorum sensing inhibition (QSI) activity of all the CuO-NS are examined using reporter strain Chromobacterium violaceum CV026 and Escherichia coli pSB1142. We found that the CuO-interlaced nanodisc structures exhibit better QSI activity than nanodiscs and leaf-shaped sheets. The interlaced nanodisc structures are inhibited various long-chain N-acyl homoserine lactones (AHLs) mediated QS individually and confirmed by other QS-associated phenomena for Pseudomonas aeruginosa, including biofilm inhibition, inhibition of virulence factors such as pyocyanin, protease production and swarming motility. Thus QSI activity of CuO-NSs is solely dependent on specific shape offering large surface area and more active sites. The CuO-NS is effective quorum sensing inhibitors, which has potential clinical applications in the management of P. aeruginosa associated infections.
A diffusion chamber technique based on spectrophotometric analysis to determine effective diffusion coefficients for solutes with various relative molecular mass (Mr) values and properties, passing through native mucus gel, is reported. For all solutes studied, a reduction in effective diffusion coefficients is observed with a retardation of solute flux by a factor of at least two. For the solutes investigated (with Mr values ranging from 126-14,400 u), no consistent effect of solutes of low Mr was evident with regard to the retarding effect of mucus; however, at high Mr values (greater than 4000 u) the retardation was greatly enhanced. A possible relationship between charged solutes of low Mr and the degree of retardation was observed, which possibly suggests the presence of ionic interactions of the solutes with the largely negatively charged mucus gel. The results provide further evidence for the suggestion that mucus is acting more than simply as a gel support for an unstirred water layer and is perhaps a more potent diffusion barrier to specific solutes. 相似文献
Tris-hydroxymethylaminomethane has been demonstrated to be an efficient organocatalyst in diversity-oriented synthesis of medicinally prevalent spirochromenes by one-pot, three-component reactions between isatins, malononitrile, and enolizable CH acids like dimedone, 4-hydroxycoumarin, 4-hydroxy-N-methylquinolin-2-one, or in situ generated 2-methylpyrazolon-2-one. Biodegradability and extremely low cost of the catalyst are the noteworthy features of this chromatography-free protocol. 相似文献
Several buffer additives that may facilitate chiral separation for optically active transition metal (TM) systems are investigated using capillary zone electrophoresis. The TM complexes evaluated exhibit considerable heterogeneity with respect to total complex charge (0 to 4+), ligand type, and identity of the central metal including Ru2+, Ni2+, Cr3+, and Co3+, threo-D[+]-Isocitrate, potassium antimonyl-d-tartrate and dibenzoyl-L-tartrate are identified as the most efficient chiral selectors. Interestingly, TM complexes exhibiting a (3+) total complex charge exhibit a reversal of enantiomer elution order versus all other complexes when separated using the tartrate additives. Operating parameters including pH, temperature, and capillary length are discussed, and chiral separations of complex mixtures are demonstrated. 相似文献
Hindered‐amine light stabilizers were surface anchored to polyethylene and polypropylene thin films by: i) direct photo‐grafting of 1,2,2,6,6‐pentamethyl piperidinyl‐4‐acrylate onto the surface and ii) by reacting of 1,2,2,6,6‐pentamethyl‐4‐piperidinol with succinic anhydride functionalized polyolefin surface. The samples were exposed to UV irradiation in air and the oxidative‐degradation of the polymers was studied with FT‐IR spectroscopy. The photo‐stability of surface anchored Hindered‐amine stabilizers (HAS) was compared with films stabilized with commercial HAS by melt blending. The results of the study evince superiority of surface anchored HAS over that of melt blended polyolefins. 相似文献
Individual rare earth impurities in high purity La2O3 (99.9%) have been determined by NAA after pre-separation of the matrix (La). The separation is carried out on an anion exchanger (Dowex 1×8) using different mixtures of methanol/nitric acid as eluants. The rare earth elements from Dy to Lu are eluted quantitatively using a 10% 1M HNO3-90% methanol mixture, while the light rare earths from Ce to Gd are eluted quantitatively using a 10% 0.05M HNO3-90% methanol mixture. La, which is retained on the column, is eluted using 0.1M HNO3. The recoveries of the various rare earth elements have been checked using radiotracers and also by spiking the sample with known amount of elements, and the recoveries are found to be quantitative. Results obtained on a typical high purity lanthanum oxide are reported here. 相似文献
In this paper, we study the synchronization behaviour of two linearly coupled parametrically excited chaotic pendula. The
stability of the synchronized state is examined using Lyapunov stability theory and linear matrix inequality (LMI); and some
sufficient criteria for global asymptotic synchronization are derived from which an estimated critical coupling is determined.
Numerical solutions are presented to verify the theoretical analysis. We also examined the transition to stable synchronous
state and show that this corresponds to a boundary crisis of the chaotic attractor. 相似文献
The dual absorptive model applied to t-channel predicts that Regge residues above threshold are In-functions which for t ? 0, for the leading trajectory, are simply Jn(Ro√−t). Thus s-channel absorptive models can be expressed entirely in terms of t-channel quantities. Implications to complex Regge poles is discussed. 相似文献