Isolation of acetic acid-tolerant Baker’s yeast variants in a turbidostat

A commercial baker’s yeast was subjected to selection in a continuous turbidostat cultivation with increasing concentration of acetic acid. The final acetic acid content in fresh medium was 0.6% or 0.8% v/v. Two of seven selected variants were stable over 15 sequential shake flask cultivations without selection pressure. After laboratory scale production of baker’s yeast, one of the variants also showed increased acetic acid tolerance in sour dough. The overall raising power (mL CO₂/h) in sour dough was improved 36%.     

Kinetics of the reactions of the IO radical with dimethyl sulfide,methanethiol, ethylene,and propylene

The reactions of IO radicals with CH₃SCH₃, CH₃SH, C₂H₄, and C₃H₆ have been studied using the discharge flow method with direct detection of IO radicals by mass spectrometry. The absolute rate constants obtained at 298 K are the following: IO + CH₃SCH₃ → products (1): k₁ = (1.5 ± 0.2) × 10^?14; IO + CH₃SH → products (2): k₂ = (6.6 ± 1.3) × 10^?16; IO + C₂H₄ →products (3): k₃ < 2 × 10^?16; IO + C₃H₆ → products (4): k₄ < 2 × 10^?16 (units are cm³ molecule^?1 s^?1). CH₃S(O)CH₃ and HOI were found as products of reactions (1) and (2), respectively. The present lower value of k₁ compared to our previous determination is discussed.     

351 nm, 0.7 ns laser damage thresholds of monomeric liquid-crystalline systems

The 351 nm laser-damage thresholds (at 0.7 nm pulse length) of monomeric liquid crystals are reported and results from aromatic-core samples are compared with those from fully saturated systems. The role of π-electron conjugation is examined and identified as the key cause for laser damage. For UV laser compatibility of devices, the damage behaviour of an alignment-layer polymer (nylon 6/6) was also investigated.     

Optimality conditions in a resource allocation problem

Translated from Vychislitel'nye Kompleksy i Modelirovanie Slozhnykh Sistem, pp. 138–141, Moscow State University, 1989.     

Synthesis of 4H-pyrans by O-cyclization of 1,5-diketones

Substituted 4H-pyrans were obtained in high preparative yields by the reaction of 1,5-diketones with acetic anhydride and boron trifluoride etherate in diethyl ether. It is assumed that the heterocyclization of 1,5-diketones includes a step involving the formation and transformations of an acyloxy carbonium ion.Translated from Khimiya Geterotsiklicheskikh Soedinenii, No. 3, pp. 320–322, March, 1992.     

Acoustic effect of pulsed high-energy proton beams

Experimental data on the generation of acoustic radiation in solid targets by pulsed high-energy proton beams are outlined. The features of ultrasound generation in solids by beams of heavy charged particles are analyzed, and the possibility of using the acoustic effect of heavy charged particles to investigate the interaction between radiation and condensed media and to determine particle energies is shown.Translated from Izvestiya Vysshikh Uchebnykh Zavedenii, Fizika, No. 8, pp. 95–99, August, 1991.     

A novel derivatization method for peptides to increase sensitivity and backbone fragmentation in liquid secondary-ion mass spectra.

Derivatization is used to increase both negative-ion sensitivity and positive-ion sequence information in the liquid secondary-ion mass spectra (LSIMS) of a series of peptides. The derivatization method involves acylation with pentafluorobenzoyl fluoride in a single-step reaction, and the reaction mixture is applied directly to the probe tip for analysis. Acylation takes place at the unprotected N-terminus, tyrosine, and lysine. The derivatives exhibit increased signal-to-noise ratio for [M-H]- ions, especially where there is not already an acidic amino acid residue in the peptide. In positive-ion LSIMS, the N-terminal group acts to retain the charge at the N-terminus, simplifying the fragmentation by producing N-terminal fragment ions. It also increases positive-ion fragmentation, sometimes very dramatically, making sequence determination more straightforward. The simplicity of the process, together with the enhancements it provides, make this a generally useful method for obtaining peptide structural information.     

Nitroazines. 20. Simple syntheses of nitropyrazolopyridines from aliphatic nitrosynthons and aminopyrazoles

Two variants of a synthesis for nitropyrazolopyridines from aminopyrazoles and two- or three-carbon nitrosynthons are examined.For Communication 19 see [1].Translated from Khimiya Geterotsiklicheskikh Soedinenii, No. 11, pp. 1560–1564, November, 1992.