Liquid chromatographic analysis of prednisolone, prednisone and their 20-reduced metabolites in perfusion media

A reversed-phase liquid chromatographic assay was developed to quantitate prednisolone, prednisone and the 20 alpha-dihydro and 20 beta-dihydro reduced metabolites of both parent compounds in tissue culture media from in vitro perfusions of the human placental lobule. Steroids were extracted from perfusate, using reversed-phase cartridges, with average recoveries of 95.2% or greater. The internal standard for the analyses was 6 alpha-methylprednisolone. In this assay cortisol coelutes with prednisolone, however, no other significant interferences were found. Assay of each steroid was linear in the range 0-1 microgram/ml. Intra-assay coefficients of variation were measured at 10 and 750 ng/ml with ranges of 3.4% (20 alpha-dihydroprednisone) to 8.8% (20 beta-dihydroprednisolone) and 4.1% (20 beta-dihydroprednisone) to 8.8% (prednisone). The corresponding inter-assay coefficients of variation were 3.3% (20 alpha-dihydroprednisone) to 9.1% (20 beta-dihydroprednisolone) and 1.9% (prednisolone) to 3.5% (prednisone). The analyses utilized two C18 columns which were linked together and maintained at 40 degrees C.     

A microeconomic simulation model of reproduction

Translated from Programmnoe Oborudovanie i Voprosy Prinyatiya Reshenii, pp. 152–184, 1989.     

On estimates of the bispectral density of certain models of random processes

Estimates are given for the bispectral densities of stationary random processes. Two models of stationary random processes with discrete time are considered. It is shown that for both models conditions hold under which one can construct a consistent estimate of the bispectral density.Translated fromTeoriya Sluchaínykh Protsessov, Vol. 14, pp. 3–7, 1986.     

Symmetries and conservation laws of Navier-Stokes equations

All the symmetries and conservation laws of Navier-Stokes equations are calculated.     

Behavior of massless feynman integrals near singular points

Nizhnetagil' State Pedagogical Institute. Translated from Teoreticheskaya i Matematicheskaya Fizika, Vol. 80, No. 3, pp. 372–380, September, 1989.     

Inner continuation of regular functions and a problem of kaufman

Translated from Ukrainskii Matematicheskii Zhurnal, Vol. 41, No. 3, pp. 408–412, March, 1989.