新型开链聚醚胺(Ⅲ)对苯并三唑N-烷基化反应的催化作用

苯并三唑N-取代基衍生物Ⅰ和Ⅱ是一类重要的杂环化合物,苯并三唑的N—H受吸电子基团—N=N—的影响,呈现较弱的酸性(pK_a=8.2)。苯并三唑在强碱作用下生成负离子,然后与烷基化剂反应,得到N-1和N-2-烷     

电解沉积成膜法制备氧化钼变色薄膜的研究

本文研究了一种新的制备过渡金属氧化钼(MoO₃)变色薄膜的方法,即电解沉积成膜法。通过X射线衍射实验证明用该方法制得的氧化钼薄膜为非晶质。与传统的真空蒸镀法相比,该薄膜同样具有良好的电致变色和光致变色特性,并观测到了可见光变色效应。结果表明,该方法将为大面积制备均匀过滤金属氧化物变色薄膜走向实用化打下基础。     

D609增强HT-29细胞对辛伐他汀的敏感性

为了探讨D609能否提高HT-29细胞对辛伐他汀的敏感性及其可能的机制,利用不同浓度的辛伐他汀处理人结肠癌细胞(HT-29)24h,利用MTT法测定并计算出IC50。随后根据上述浓度将HT-29细胞随机分为四组:对照组、D609组、辛伐他汀组和D609+辛伐他汀组,加药处理24h后,采用MTT法检测D609和辛伐他汀对HT-29细胞增殖的影响,实时荧光定量PCR(qRT-PCR)和蛋白质印迹(WB)检测各组RIP140、SMS1、SMS2、BCL-2和BAX的表达。MTT实验结果显示,辛伐他汀可明显抑制HT-29细胞生长,且呈剂量依赖效应,其IC50为:67.98μmol·L-1,而辛伐他汀和D609联用可明显增强辛伐他汀对HT-29的生长抑制作用(P<0.05,n=3)。qRT-PCR和WB实验结果表明D609和辛伐他汀处理均上调了RIP140及BAX的表达,但降低了SMS1、SMS2、BCL-2的表达(P<0.05,n=3),而两者联用可显著增强这些改变(P<0.05,n=3),即D609可提高HT29细胞对辛伐他汀的敏感性,其机制可能与D609通过抑制SMS的活性和增加RIP140的表达有关。     

Numerical solutions of singular integral equations for planar rectangular interfacial crack in three dimensional bimaterials

Stress intensity factors for a three dimensional rectangular interfacial crack were considered using the body force method. In the numerical calculations, unknown body force densities were approximated by the products of the fundamental densities and power series; here the fundamental densities are chosen to express singular stress fields due to an interface crack exactly. The calculation shows that the numerical results are satisfied. The stress intensity factors for a rectangular interface crack were indicated accurately with the varying aspect ratio, and bimaterial parameter.     

Studies on high-moment soft magnetic FeCo/Co thin films

The dependences of soft magnetic properties and microstructures of the sputtered FeCo (=FeFeCo薄膜 溅射条件 软磁性 高饱和磁化强度FeCo film, sputtering conditions, high saturation magnetization, soft magnetic properties2005-10-263/7/2006 12:00:00 AMThe dependences of soft magnetic properties and microstructures of the sputtered FeCo （=Fe65Co35） films on Co underlayer thickness tCo, FeCo thickness tFeCo, substrate temperature Ts and taxget-substrate spacing dT-s are studied. FeCo single layer generally shows a high coercivity with no obvious magnetic anisotropy. Excellent soft magnetic properties with saturation magnetization μ0Ms of 2.35 T and hard axis coercivity Hch of 0.25 kA/m in FeCo films can be achieved by introducing a Co underlayer. It is shown that sandwiching a Co underlayer causes a change in orientation and reduction in grain size from 70 nm to about 10 nm in the FeCo layer. The magnetic softness can be explained by the Hoffmann＇s ripple theory due to the effect of grain size. The magnetic anisotropy can be controlled by changing dT-S, and a maximum of 14.3 kA/m for anisotropic field Hk is obtained with dT-S=18.0 cm.     

高效液相色谱法对黄精多糖相对分子质量及组成的测定

从内蒙古野生黄精根茎中提取出粗黄精多糖,并用胰蛋白酶法和Sevag法结合脱去其中的蛋白质,采用高效液相色谱法,利用Shodex GPC色谱柱对其纯度、相对分子质量和相对分子质量的分布进行了测定,结果表明所得产物为均一成分,测得的数均相对分子质量（Mn）为7073,相对分子质量（Mr）为7247,分散系数为1.025。以水为流动相,利用Shodex SP0810糖色谱柱对黄精多糖的组成进行分析,结果表明,黄精多糖是由单一的果糖组成的。     

水溶液中碳纳米管的表面增强拉曼光谱研究

合成了碳纳米管和金纳米颗粒的复合物, 测量了水溶液相中复合物的表面增强拉曼光谱, 结果表明, 碳纳米管的巯基化修饰可以提高碳纳米管与金纳米颗粒复合的效率, 随着金纳米颗粒负载量的增加, 碳纳米管的拉曼信号逐渐增强. 加入己二胺分子可以减小金纳米颗粒之间的距离使表面增强效应更显著, 碳纳米管的拉曼光谱得到进一步的增强. 还可进一步在复合体系中加入对巯基苯胺和罗丹明B等小分子拉曼探针, 利用金纳米颗粒的表面增强效应, 这种多元复合体系有望作为多通道拉曼成像探针材料.     

双链特异性核酸切割酶等温循环扩增结合 高效液相色谱高灵敏检测双目标microRNA

通过双链特异性核酸切割酶(DSN)循环扩增策略结合高效液相色谱(HPLC)法,实现了对两种疾病标志物microRNA(miRNA)的选择性分离和高灵敏检测.采用酶循环等温扩增策略增强了目标miRNA的信号,提高了HPLC法检测核酸的灵敏度.利用固定在磁珠上的不同长度和碱基序列的DNA探针实现了不同miRNA信号的色谱分...     

Study of BESⅢ MUC offine software with cosmic-ray data

Cosmic-ray data of 90 M events have been collected and used for calibration,alignment as well as detector tuning. A special tracking algorithm for the BESⅢ muon counter is developed and verified with Monte-Carlo simulation and then further confirmed with the cosmic-ray data. The obtained strip resolutions are in good agreement with the design values. A new alignment approach for the BESⅢ muon counter is confirmed with the cosmic-ray data and proposed to be used in future analysis of experimental data.