悬浮固化分散液液微萃取技术的发展及应用进展

综述了悬浮固化分散液液微萃取技术的发展,及其在水、食品和生物样品分析中的应用进展,并对其前景作了简要展望(引用文献67篇)。     

Preparation and Characterization of BPO Film as Electrode for Using of FeRAM

Conductive perovskite BaPb03 （BPO） films as a potential electrode material of PZT capacitors used in ferroelectric random access memory are prepared by rf magnetron sputtering. An x-ray diffractometer and standard four probe method are employed to investigate the dependence of growth conditions on crystal structure and conductivity of BPO films. It is found that BPO films with perovskite phase can be obtained at substrate temperatures above 425℃, and the sample with the lowest resistivity is obtained at 450℃ under pure argon atmosphere. Using this BPO film as electrode, ferroelectric properties of BPO/PZT/BPO and Pt/PZT/BPO sandwiched structures are evaluated. Their remanent polarization and coercive field are 36.6 ℃/cm^2 （81.3 k V/cm） and 36.9℃/cm^2 （89.1 kV/cm）, respectively. The coercive field of the former structure is lower than that of the latter, but remanent polarizations are almost the same. In addition, the results imply that BPO electrode is helpful to improve the fatigue resistance of PZT. The reasons are discussed.     

不同pH下人血清白蛋白热变性及结构可逆性研究

本文通过用差示扫描量热法(DSC)、动态光散射(DLS)和圆二色谱(CD)检测不同p H下人血清白蛋白(HSA)的结构,并观察其随p H变化的可逆性,以探讨不同p H对HSA结构的影响及HSA结构在p H变化下的可逆性。结果表明,p H7.0条件下HSA的热稳定性最好,热变性温度(Tm)为68.17±0.06℃,不同p H条件下HSA三级结构均发生了一定程度的改变,酸性和碱性条件下的HSA调回p H7.0后变性温度接近于p H7.0条件下的Tm值。不同p H条件下的粒径相对于p H7.0条件下增大,调回p H7.0后减小。CD实验表明了p H对HSA二级结构的影响。HSA结构在不同p H下调回中性条件时会重新折叠,但不能恢复中性条件下的结构状态。     

聚苯胺复合材料的制备方法及应用进展

复合材料是由两种或多种性质不同的物质组成的多元材料,具有比各组分材料更优异的或原来不具备的性能。聚苯胺(PANI)作为最具应用前景的导电高分子材料,国内外对基于PANI的复合材料的研究也愈加关注。本文对PANI复合材料制备方法的研究成果进行了综述,主要包括原位化学法、共混法、电化学法、层层自组装法等。探讨了PANI复合材料在电极、导电、防腐、传感、分离、催化等方面的应用性能,并展望了PANI复合材料今后研究与应用的发展方向。     

间隙线性反馈控制混沌

提出了两种控制混沌的线性间隙反馈方法.该方法由控制相和非控制相组成,通过选取合适的反馈系数和控制相时间,可以获得各种不同的所需稳定的周期轨道.分别对一维的声光双稳系统和二维的类Henon吸引子进行计算机模拟,表明该方法可以使既定的系统按照给定的周期轨道演化,并且是大范围可控的. 关键词： 间隙线性反馈 混沌 类Henon映射 Lyapunov指数     

Effects of drive imbalance on the particle emission from a Bose–Einstein condensate in a one-dimensional lattice

Time-periodic driving has been an effective tool in the field of nonequilibrium quantum dynamics, which enables precise control of the particle interactions. We investigate the collective emission of particles from a Bose–Einstein condensate in a one-dimensional lattice with periodic drives that are separate in modulation amplitudes and relative phases. In addition to the enhancement of particle emission, we find that amplitude imbalances lead to energy shift and band broadening,while typical re...     

蛋白质的化学修饰策略

蛋白质的化学修饰反应是在维持蛋白质的完整性与功能的基础上,通过官能团的化学反应获得新的生物缀合物。本文综合分析了蛋白质结构以及反应条件对化学修饰反应的影响,分类评述了蛋白质化学修饰的4种主要方法(氨基修饰、巯基修饰、羧基修饰以及苯羟基修饰)的机理、适用范围以及实际操作中的优劣,并探讨了修饰反应目前面临的问题以及未来发展的方向。     

Measurements of the Cross-sections of Produced Short-Lived Nuclei Induced by Neutrons around 14 MeV on Isotopes of Tungsten

New experimental cross-section data for the180 W(n,2 n)179 mW,186 W(n,2 n)185 mW and186 W(n,p)186 Ta reactions at the neutron energies of 13.5 and 14.4 MeV are obtained by the activation technique. The neutron beams are produced by means of the3 H(d,n)4 He reaction. The gamma activities of the product nuclei are measured by a high-resolution gamma-ray spectrometer with a coaxial high-purity germanium detector. The neutron fluence is determined using the monitor reaction93 Nb(n,2 n)92 mNb. The results in the current work are discussed and compared with the measurement results found in the literature. It is shown that these higher accuracy experimental cross-section data around the neutron energy of 14 MeV agree with some previous experimental values from the literature within experimental uncertainties.     

干旱胁迫对长雄野生稻根际微生物群落结构的影响

干旱胁迫是影响水稻生产的主要障碍。本研究以6种长雄野生稻(Oryza longistaminata)为材料,采用盆栽控水模拟干旱胁迫,通过16SrDNA高通量测序技术,研究干旱胁迫对长雄野生稻根际微生物群落结构的影响。结果表明:细菌的优势门是变形菌门(Proteobacteria)、绿弯菌门(Chloroflexi)和酸杆菌门(Acidobacteria),相对丰度分别为24.3%～36.49%、11.94%～20.39%和8.9%～17.83%。古菌的优势门是泉古菌门(Crenarchaeota)和广古菌门(Euryarchaeota),相对丰度分别为0.50%～1.05%,0.17%～0.64%。与空白土壤比较,干旱胁迫后的根际土壤微生物菌群多样性未发生明显变化,但群落组成结构发生显著改变(P<0.05)。在干旱胁迫处理下,酸杆菌门(Acidobacteria)、α-变形菌(Alphaproteobacteria)、疣微菌门(Verrucomicrobia)、放线杆菌门(Actinobacteria)、TM7和泉古菌门(Crenarchaeota)的相对丰度显著升高,而δ-变形菌(Deltaproteobacteria)、硝化螺旋菌门(Nitrospirae)、OD1、绿菌门(Chlorobi)、螺旋菌门(Spirochaetes)、OP11和NC10的相对丰度显著降低。这些结果表明,长雄野生稻根际微生物能对干旱环境进行响应,发生群落结构改变达到新的平衡,保持同样高的群落结构多样性,来应对干旱环境的变化。     

GC-MS测定血清胆固醇及其标志物前处理条件正交试验优化研究

采用正交试验优化GC-MS测定血清胆固醇及其标志物的样品前处理条件,并对最优试验方案进行方法学评价。采用L₁₆(211)正交设计对影响GC-MS测定血清胆固醇及其标志物前处理的3个关键步骤共7个因素——皂化(KOH-乙醇浓度、皂化温度和时间)、萃取(正己烷用量)和衍生化(衍生化温度、时间和用量)进行优化。得到最优前处理条件组合如下：KOH-乙醇溶液浓度为1 mol·L-1;皂化温度70 ℃;皂化时间60 min;萃取剂用量2 mL;衍生化温度70 ℃;衍生化时间60 min;衍生化试剂用量100 μL。所建方法准确性和精密性良好,方法学评价指标优于文献报道。