MALIGNANT TRANSFORMATION OF HUMAN FETAL LUNG FIBROBLASTS INDUCED BY NITROSAMINE COMPOUNDS IN VITRO

This report, for the first time, demonstrates that diethyl-nitrosamine (NDEA) and a new nitrosamine compound-N-l-methylacetonyl-N-3-methylbutyl-nitrosamine (MAMBNA), which are suspicious carcinogens of esophageal cancer (EC) in Linxian, Henan Province, are able to cause the malignant transformation (altered morphology, extended life span, anchorage independent growth, invasiveness, tumorigenicity, etc.) of normal diploid fibroblasts derived from human fetal lung. Besides, the preselection for clonogenic cells, which seem to be more readily to be transformed, in soft agar before carcinogen treatment, could be considered as one of the useful ways in establishment of a model system of malignant transformation of human fibroblasts in vitro.     

EB病毒进入非B淋巴细胞的机理研究——一株可产生EB病毒的人和树鼩的杂交细胞株的建立

本文用可产生EB病毒的类淋巴母细胞株H_(18)与正常树(鼠句)鼻咽部组织培养单层细胞共同培养,建立了一株能产生EB病毒的人和树(鼠句)的杂交细胞株——TuH_(18),讨论了EB病毒进入非B淋巴细胞的机理,研究了TuH_(18)病毒的生物学和生物化学特性。