Fluorimetric determination of diacetyl and 2,3-pentanedione with isoniazide and a zirconium salt

Diacetyl and 2,3-pentanedione react with isoniazide in acidified solutions to give the corresponding hydrazones which, by reacting with a zirconium (IV) salt, form fluorescent complexes. Both reactions have been studied separately. Variables such as pH, heating time, and excess of reactives are discussed. The method may be used for the determination of these compounds in foods.     

Polarization of the Electrical Double Layer. Time Evolution after Application of an Electric Field

Electrophoresis is one of the electrokinetic phenomena most widely investigated, both from a fundamental point of view and as a research tool in academia and industry. However, the dependence between electrophoretic mobility and zeta potential is, in a general case, far from simple, because of the many physical processes involved. In this work, we first describe qualitatively and (in some cases) quantitatively the time behavior of the dipole moment induced in the electrical double layer by an applied electric field. Further, a simple relationship is deduced between the dipole moment and the electrophoretic mobility. Through the analysis of the time dependence of the former, it is possible to resolve the different contributions to the stationary values of the mobility. Three characteristic relaxation times are distinguished in the time evolution of the dipole moment: tau(H) (the time needed for hydrodynamic flows to be established), tau(MW) (time for ionic electromigration to develop), and tau(VD) (after this time, diffusion flows are established in the system, and the double layer polarization is complete). This means that different mechanisms are operating on the double layer for different times after the application of the field, and that computing the mobility at such different times is equivalent to calculating the steady-state electrophoretic mobility under different approximations. A comparison is shown between estimated and computed mobility values as functions of time and of zeta potential, confirming the validity of the asymptotic calculations. Copyright 2000 Academic Press.