两个四唑配合物的原位合成、多样化配位模式和强荧光性质

在路易斯酸ZnCl₂或MnSO₄·7H₂O作用下,通过1-甲基-1-氢-咪唑-4,5-二甲腈与NaN₃水热原位合成了2个四唑配合物：{[Zn₂（midt）（Hmidt）]（N₃）·H₂O}_n （1）和[Mn（midt）₂·（H₂O）₂]·H₂O （2）（midt=1-甲基-1-氢-咪唑-4,5-二四唑）。X射线单晶衍射表明尽管配合物1和2均结晶于同样的P1 空间群,但他们有完全不同的结构。配合物1为一个有趣的二维聚合物结构,含有两个不同配位环境的锌原子和多种配位模式的midt配体,而配合物2为一个三维超分子结构,包含一个有趣的水分子链结构。固态下1和2分别在353和382 nm处显示出较强的蓝色荧光。     

用于目标跟踪/识别中红外AOTF光谱相机光学系统的设计与优化

为获得红外目标的空间信息和光谱信息,设计了一种用于中波红外声光可调谐光谱相机的光学系统.设计中引入折/衍射混合透镜,在用6片透镜的情况下达到了12×变倍,在长焦处时能识别2 000m远的目标.采用ZEMAX光学设计软件模拟声光晶体的衍射,在晶体出射面上优化设计了4°的楔角,使-1级衍射光能共轴出射.该光学系统在空间频率17lp/mm处调制传递函数值最大达到0.7,能量集中度大于90%,能够满足光谱相机用于识别/跟踪目标的要求.     

Particle captured by a field-modulating vortex through dielectrophoresis force

In microfluidic technology, dielectrophoresis (DEP) is commonly used to manipulate particles. In this work, the fluid-particle interactions in a microfluidic system are investigated numerically by a finite difference method (FDM) for electric field distribution and a lattice Boltzmann method (LBM) for the fluid flow. In this system, efficient particle manipulation may be realized by combining DEP and field-modulating vortex. The influence of the density ($\rho_{\rm p}$), radius ($r$), and initial position of the particle in the $y$ direction ($y_{\rm p0}$), and the slip velocity ($u_{0}$) on the particle manipulation are studied systematically. It is found that compared with the particle without action of DEP force, the particle subjected to a DEP force may be captured by the vortex over a wider range of parameters. In the $y$ direction, as $\rho_{\rm p}$ or $r $ increases, the particle can be captured more easily by the vortex since it is subjected to a stronger DEP force. When $u_{0}$ is low, particle is more likely to be captured due to the vortex-particle interaction. Furthermore, the flow field around the particle is analyzed to explore the underlying mechanism. The results obtained in the present study may provide theoretical support for engineering applications of field-controlled vortices to manipulate particles.     

Parasitic bipolar amplification in a single event transient and its temperature dependence

Using three-dimensional technology computer-aided design (TCAD) simulation, parasitic bipolar amplification in a single event transient (SET) current of a single transistor and its temperature dependence are studied. We quantify the contributions of different current components in a SET current pulse, and it is found that the proportion of parasitic bipolar amplification in total collected charge is about 30% in both 130-nm and 90-nm technologies. The temperature dependence of parasitic bipolar amplification and the mechanism of the SET pulse are also investigated and quantified. The results show that the proportion of charge induced by parasitic bipolar increases with rising temperature, which illustrates that the parasitic bipolar amplification plays an important role in the charge collection of a single transistor.     

Analysis and finite element simulation of electromagnetic heating in the nitride MOCVD reactor

Electromagnetic field distribution in the vertical metal organic chemical vapour deposition (MOCVD) reactor is simulated by using the finite element method (FEM). The effects of alternating current frequency, intensity, coil turn number and the distance between the coil turns on the distribution of the Joule heat are analysed separately, and their relations to the value of Joule heat are also investigated. The temperature distribution on the susceptor is also obtained. It is observed that the results of the simulation are in good agreement with previous measurements.     

聚乳酸组织工程支架材料

综述了生物活性因子固定化的聚乳酸-聚氨基酸衍生物共聚物和通过亲-疏水性设计的众多聚乳酸-聚氧化乙烯（PLA-PEO）共聚物的研究进展。展现了其在组织工程材料,药物控释体系和其他生物医用材料中的广泛应用前景。     

毕赤酵母表达的HIV-2外膜糖蛋白的纯化与活性测定

人免疫缺陷病毒(Human immunodeficiency virus,HIV)是艾滋病（AIDS）的病原体,其外膜蛋白是HIV侵入人体靶细胞的物质基础,对其结构与功能的深入研究不但能为彻底揭示HIV致病机理奠定基础,而且有助于抗感染免疫研究和治疗物的设计^[1,2],HIV－2ROD外膜蛋白gp105是一种高度糖基化的糖蛋白,近年来发展迅速的毕赤酵母（Pichia Pastoris）真核表达系统对分泌蛋白的糖基化修饰形式与天然的gp105相同^[3]。为实现大量制备有活性的gp105,我们用PCR法去除了gp105基因5′端28个密码子的非功能片段,以提高gp105的表达水平,并成功地在毕赤酵母中实现了gp105的高效分泌表达。本文对这一表面产物的纯化、性质及活性进行了研究,为尽快研制出有效的疫苗奠定基础。     

用界面几何约束的多起点蒙特卡罗方法对蛋白质/ 短肽识别体系的对接计算

提出了一个界面几何约束的多起点蒙特卡罗构象搜索方法,并把这个方法用于三个丝氨酸蛋白酶/短肽抑制剂体系的刚性、部分柔性和全部柔性对接计算中。我们的方法成功地预测出了接近晶体结构的配体构象。与没有几何约束相比,我们的几何约束蒙特卡罗方法显示出了更好的收敛性质。     

确定蛋白质-短肽复合物结构的新方法

大部分蛋白质 -蛋白质复合物的三维结构在接触表面都显示出很好的几何匹配 .由于蛋白质的表面几何形状和其它的一些物理化学性质在分子的专一性相互作用中起了主要作用 ,所以 ,接触表面几何形状的互补常常被认为是蛋白质分子识别的基础 .一般来说 ,蛋白质接触表面的几何匹配只涉及 5到 1 0几个紧密堆积的氨基酸残基 ,因此 ,蛋白质与蛋白质配体之间的识别计算可以通过蛋白质与突变周围的或与蛋白质表面紧密接触的配体肽段的识别计算来实现 . Stoddard等 [1] 已经利用从 MBP上选取的八肽成功地计算出接近晶体结构的 MBP-受体复合物 .许多研…