创新性物理实验模式初探

主要参考了国内外高校物理实验模式,将创新性模式与传统实验模式、综合性、设计性实验模式和研究性实验模式进行比较,提出创新性实验模式实施的可行性和必要性,突出实验模式在培养学生创新意识和增强学生能力方面的优势.     

铽与1-苯基-3-甲基-4-酰代吡唑啉-5-酮的三元配合物的合成、表征与荧光性能

合成了铽与1-苯基-3-甲基-4-异丁酰基吡唑啉-5-酮(HPMIBP)、1-苯基-3-甲基-4-苯甲酰基吡唑啉-5-酮(HPMBP)的四个三元配合物Tb(PMIBP)3.2H2O(A1), Tb(PMIBP)3.bpy(A2), Tb(PMBP)3.2H2O(B1)和Tb(PMBP)3.bpy(B2)(bpy=2, 2'-联吡啶)。用元素分析确定了它们的组成, 并用紫外-可见光谱、红外光谱、差热-热重谱对其进行了表征。研究了它们在固态和溶液中的荧光光谱, 并用频域法测定了它们在溶液中的荧光寿命, 结果表明A1和A2的荧光强度比相应的B1和B2强三个数量级, A2与A1或B2与B1相比, 荧光强度也有一定程度的增强,并且不同溶剂对其荧光强度和荧光寿命都有较大的影响。     

高效液相色谱双波长检测法测定维C银翘片中4种组分的含量

建立了高效液相色谱测定维C银翘片中维生素C、对乙酰氨基酚、马来酸氯苯那敏和绿原酸4种组分含量的方法。研究优化了样品提取的溶剂与超声提取条件、色谱分离条件和检测波长等,采用了Sinochrom ODS-BP(4.6 mm×200 mm, 5 μm)色谱柱,以0.05 mol/L KH2PO4(pH 3.0,含1%三乙胺)-乙腈(75:25, v/v)为流动相,双检测波长260 nm和326 nm等分离检测条件。结果表明,维生素C、对乙酰氨基酚、马来酸氯苯那敏和绿原酸的测定线性范围均比较宽,方法平均回收率大于99.4%,相对标准偏差(RSD)小于1.8%(n=5),而且测定方法快速、简便、准确,非常适合维C银翘片类药物的质量控制。     

2006年诺贝尔化学奖成果简介

简要介绍了2006诺贝尔化学奖的成果,获奖者美国科学家罗杰.科恩伯格在该成果的主要贡献在于对真核转录过程的分子研究,对于人们理解转录过程具有深远的意义。目前,基因转录的过程广泛应用在基因研究的实验室中。     

应用固相萃取技术分析主流烟气中的芳香胺

建立了一种定量检测卷烟主流烟气中的芳香胺的新方法:应用第一柱为阳离子交换保留机理而第二柱为吸附保留机理的固相萃取(SEE)净化程序来处理样品,从第一柱上洗脱的芳香胺用五氟丙酸酐进行衍生化后,进入第二柱吸附色素和其它杂质,利用GC/MS进行分析测定.该方法具有灵敏度高、准确度高和易于自动化等优点,检出限为0.09ng/cig.～1.26ng/cig.,回收率为76.4%～103.1%.用该方法测定了某品牌卷烟烟气总粒相物中的14种芳香胺.     

A New Pregnane Glycoside from Dioscorea collettii var. hypoglauca

Morphologicaldeformationsofthemyceliaorconidiaofmicroorganismssuchascurling,swelling,hyper-divergence,beadformationandinhibitionofgermination,areofteninducedinthepresenceofbioactivesubstances".AnewscreeningbioassaymethodfordetectingthedeformationofmyceliagerminatedfromconidiaofPyriculartaoryzaeP-2bwasfirstdevelopedforthequantitativeapplicationtoscreeningantifungalandantineoplasticagentsbyH.Kobayashi'.Thisbioassayisquick,easytoperform,andhasbeenefficientlyusedinthepreliminaryscreeningofantineo…     

Experimental study of the Plasma Fluorination of Y—Ba—Cu—O Thin Films

We have experimentally studied the surface mjodifications of Y-Ba-Cu-O (YBCO) thin films using CF4 plasma.The intensity of the plasma fluorination was controlled by controlled by changing the biasing voltage and the time of the plasma treatment.Microstructural analyses reveal that the oxygen content of the YBCO thin films was changed.Transport measurements of sufficient fluorinated YBCO films imply that the films changed totally into an oxygen-deficient semi-conducting state.From these experimental results,we believe that plasma fluorination is quite a useful method to form controllable a thin barrier layer in fabricating interface engineered junctions and to form a stable narrow weak-link region in fabricating planar superconductor-normal-superconductor junctions.     

污染水产去甲基化表观遗传毒性EGFP评价方法

pEGFP-C3质粒经过体外人工甲基化处理后,被转染进入HepG2细胞以构建重组细胞株．以5-AZA为阳性去甲基化毒物与重组细胞共培养,通过亚硫酸氢钠测序法定量检测EGFP基因启动子区甲基化状态,通过实时定量PCR检测EGFP基因表达,借助流式细胞术和荧光摄片定量检测共培养细胞的绿色荧光强度,在DNA甲基化、EGFP基因mRNA表达、GFP蛋白等多个层次研究5-AZA染毒处理与其去甲基化能力和荧光表达改变的响应关系．对天津污染水产的去甲基化能力进行了实际样品测试．结果表明,5-AZA与重组细胞的DNA甲基化、基因表达、蛋白产物变化之间存在显著关联,具有较低的检出浓度和良好的重复性．天津污染海域的水产去甲基化能力较强．本文初步建立了一种污染物去甲基化表观遗传毒性评价方法．     

Discovery and application of 6π-azaelectrocyclization to natural product synthesis and synthetic biology

While elucidating the inhibitory mechanism of a hydrolytic enzyme by aldehyde-containing natural product,we discovered a reaction involving a rapid 6π-azaelectrocyclization of azatrienes generated from aldehyde with lysine residues.The electrocyclic reaction of the 1-azatriene system,a cyclization precursor,exhibited a significant substituent effect.Asymmetric chiral piperidine synthesis and a one-pot library synthesis of pyridines on solid-supports were applied to synthesize pyridine/indole alkaloid-type natural product.Additionally,we developed lysine-based labeling and engineering of biomolecules and living cells based on the rapid 6π-azaelectrocyclization.Both labels and oligosaccharide structures were introduced efficiently and selectively into surface lysines under the mild conditions;notable effects of N-glycans on proteins and living cells were visualized for the first time by PET and noninvasive fluorescence imaging.     

An improved synthesis of (-)-7-isopropyl-cis-l-amino-2-indanol the chiral auxiliary for 6π-azaelectrocyclization

(-)-7-Isopropyl-cis-1-amino-2-indanol,a key chiral auxiliary and ligand in the highly stereo-selective asymmetric 6π-azaelectrocyclization, has been prepared previously by two methods.Each however involved using of one extreme condition, i.e.high temperature or high pressure,for the respective reaction.A modified reaction route employed mild condition for synthesis was presented in this report.