EPR investigation of cell membrane fluidity upon external oxidative stimulus

The perturbation of the physical state of cell membranes triggered by an external oxidative stimulus has been studied with sperm cells which were chosen as a model system. Electron paramagnetic resonance (EPR) spectroscopy was applied and three different nitroxides bearing a paramagnetic group on the 5th, 7th and 13th carbon of the stearic acid acyl chain were used to probe different regions of the membrane. The theoretical simulations of the experimental spectra indicate distinct domains in the intact cell plasma membrane where local steric constraints impose different degrees of motional averaging experienced by the reporter group. Upon the external oxidative stimulus the spectral changes were proportional to the accumulated oxidation products and were detected only for the reporter group residing close to the lipid-water interface. The EPR spectra reveal that the motion of the reporter group has slowed down in the oxidized cells and that the oxidatively modified membrane shows up as a more rigid structure as compared with the native state. The results also indicate that the oxidation-induced spectral changes are supressed in the presence of gangliosides.     

A multivalued version of Sharkovskiĭ’s theorem holds with at most two exceptions

A multivalued version of Sharkovskiĭ’s theorem is formulated for M-maps on linear continua and, more generally, for triangular M-maps on a Cartesian product of linear continua. This improves the main result of [AP1] in the sense that our multivalued analogue holds with at most two exceptions. A further specification requires some additional restrictions. For instance, 3- orbits of m-maps imply the existence of k-orbits for all k ? \mathbbNk \in {\mathbb{N}} , except possibly for k ?k \in {4, 6}. It is also shown that, on every connected linearly ordered topological space, an M-map with orbits of all periods can always be constructed. This demonstrates that Baldwin’s classification of linear continua in terms of admissible periods [Ba] is useless for multivalued maps.     

Influence of boric acid on the electrochemical deposition of Ni

The electrolytic deposition of Ni onto a paraffin-impregnated graphite electrode from supporting chloride electrolyte (0.5 mol dm⁻³ NaCl) adjusted to the required pH using dilute HCl is investigated. The effect of electrolyte composition on the Ni electrodeposition is studied using linear sweep voltammetry in the cathodic region. An elimination voltammetry procedure was applied to evaluate the polarization curves. The aim of this work was to deduce the mechanism of Ni reduction in the chloride bath as well as the influence of boric acid on this. Positively-charged NiCl⁺ ions were found to be the electroactive particles in the Ni reduction mechanism. The strong competition between the NiCl⁺, Cl⁻ and H⁺ ions for active sites at the electrode is discussed. Kinetically-controlled adsorption/desorption processes of various species were also confirmed using elimination voltammetry with a linear scan. The evolution of gaseous hydrogen, catalyzed by the freshly-deposited Ni, accompanies the electrodeposition process. The presence of boric acid at a sufficiently high concentration inhibits the deposition of Ni and, at the same time, improves the morphology and brightness, as well as the adhesion of the deposited Ni. Elimination voltammetry with a linear scan is an efficient way to evaluate current–potential curves that reflect the electrodeposition of one-component Ni coatings. By eliminating selected currents, additional interesting and useful information can be obtained from voltammetric data.     

On the 2-rainbow domination in graphs

The concept of 2-rainbow domination of a graph G coincides with the ordinary domination of the prism G□K₂. In this paper, we show that the problem of deciding if a graph has a 2-rainbow dominating function of a given weight is NP-complete even when restricted to bipartite graphs or chordal graphs. Exact values of 2-rainbow domination numbers of several classes of graphs are found, and it is shown that for the generalized Petersen graphs GP(n,k) this number is between ⌈4n/5⌉ and n with both bounds being sharp.     

Symplectic Generating Functions and Moyal Products

We introduce an affine-invariant version of generating functions of symplectic transformations of affine symplectic spaces, together with a generalization for other symmetric symplectic spaces. The composition of these functions has a nice connection with the Moyal product.     

A simple model of thin-film point contact in two and three dimensions

We treat a free spinless quantum particle moving on a configuration manifold which consists of two identical parts connected in one point. Most attention is paid to the three-dimensional case when these parts are halfspaces with Neumann condition on the boundary; we also discuss briefly a more general boundary conditions. The class of admissible Hamiltonians is constructed by means of the theory of self-adjoint extensions. Among them, particularly important is a two-parameter family whose elements are invariant with respect to exchange of the halfspaces; we compute the transmission coefficient for each of these extensions. We discuss also the motion on two planes considered in our recent paper, obtaining another characterization of the admissible Hamiltonians. In conclusion, the two situations are compared as models for point-contact spectroscopical experiments in thin metal films.On leave of absence fromNuclear Physics Institute, Czechosl. Acad. Sci., 250 68 e near Prague, Czechoslovakia.On leave of absence fromNuclear Centre, Charles University, V Holeovikách 2, 180 00 Prague 8, Czechoslovakia.