单颗粒计数策略定量检测癌胚抗原

该文提出了一种基于单颗粒电感耦合等离子体质谱(spICP-MS)的计数策略,用于癌胚抗原(CEA)的灵敏检测.该策略采用50 nm的金纳米颗粒(AuNPs)作为免疫识别和单颗粒计数的探针,以磁性纳米颗粒(MBs)作为免疫识别的捕获中心,基于夹心免疫反应,应用抗体标记的MBs捕获CEA和AuNPs探针.通过磁铁分离后,捕...     

生物组织多光谱偏振特性研究

生物组织内部结构复杂且具有较强的散射特性,而光作为生物组织检测的重要信息载体,其自身特性包括颜色、幅值、偏振等都对信息获取有较大的影响.结合偏振成像,对生物组织多光谱偏振特性展开了研究,依据不同微粒尺寸的分布建立了均匀单层生物组织模型,结合瑞利和米氏散射理论模拟了基于单个微粒的两种散射事件.瑞利散射具有较好的前向后向散...     

Batch process monitoring and its application to polymerization systems

Slight changes in raw material properties or operating conditions during critical periods of operation of batch and semi-batch polymerization reactors may have a strong influence on reaction mechanism and impact final product quality. Online process monitoring, fault detection, fault diagnosis, and product quality prediction in real-time ensure safe reactor operation and warn operators about excursions from normal operation that may lead to deterioration in product properties. Multivariate statistical process monitoring and quality prediction using multiway principal components analysis and multiway partial least squares have been successful in detecting abnormalities in process operation and product quality. When abnormal process operation is detected, fault diagnosis tools are used to determine the source cause of the deviation. Illustrative case studies are presented via simulated polyvinyl acetate polymerization.     

超重力作用下软材料薄膜失稳的有限元分析

软材料在自然界和工业应用中无处不在,其显著特点是对外界刺激极其敏感,相对于传统材料其弹性模量更小,容易发生表面形态的失稳现象．现有研究主要把光、声、电等作为诱导材料失稳的因素进行研究,而重力往往作为常量考虑．本文通过有限元模拟,建立超重力作用下环向受限的圆柱状软材料薄膜失稳模型,探究了薄膜材料的密度、厚度、半径、剪切模量等材料和几何参数对失稳临界超重力和临界失稳模态的影响．结果表明,触发薄膜失稳的超重力随薄膜厚度和密度增大而减小,随剪切模量增大而增大,随着薄膜半径增大快速减小并迅速趋于常数．另外,当径厚比R/H较小时,无量纲临界超重力随着径厚比增大而单调减小,但当径厚比R/H大于3 时,此量为一个常数．超重力作用下软材料的临界失稳模态与密度、剪切模量等参数无关,随径厚比R/H增大,临界失稳模态波数增大、波长减小．     

INTRODUCTION TO THE 7TH TEACHING COMPETITION OF YOUTH FACULTY OF FUNDAMENTAL MECHANICS COURSES

阐述了教育部高等学校力学基础课程教学指导分委员会举办的全国高校第七届基础力学青年教师讲课比赛的基本情况、本届讲课比赛的特点、讲课比赛中反映出的问题及其改进的建议。希望通过本文的介绍,让国内各高校教师了解本届讲课比赛以及比赛中反映出的问题,为进一步办好讲课比赛、提高我国基础力学课程的教学水平和质量提供参考。     

In vitro synergistic efficacy of conjugated linoleic acid,oleic acid,safflower oil and taxol cytotoxicity on PC3 cells

The aim of this study was to determine in vitro synergistic efficacy of conjugated linoleic acid (CLA), oleic acid (OLA), safflower oil and taxol (Tax) cytotoxicity on human prostate cancer (PC3) cell line. To determine synergistic efficacy of oil combinations, PC3 treated with different doses of compounds alone and combined with 10 μg/mL Tax. The MTT results indicated that OLA–Tax combinations exhibited cytotoxicity against PC3 at doses of 30 nM+10 μg-Tax, 15 nM+5 μg-Tax and 7.5 nM+2.5 μg-Tax. The treatment of OLA or Tax did not show significant inhibition on PC3, while OLA–Tax combinations showed effective cytotoxicity at treated doses. CLA–Tax combinations demonstrated the same effect on PC3 as combined form with 45.72% versus the alone form as 74.51% viability. Cytotoxic synergy between Tax, OLA and CLA shows enhanced cytotoxicity on PC3 which might be used in the therapy of prostate cancer.     

Impact of Capillary Pressure, Salinity and In situ Conditions on CO2 Injection into Saline Aquifers

This article presents a numerical investigation of the combined effects of capillary pressure, salinity and in situ thermodynamic conditions on CO₂-brine-rock interactions in a saline aquifer. We demonstrate that the interrelations between capillary pressure, salinity, dissolution and drying-out affect CO₂ injectivity and storage capacity of a saline aquifer. High capillary forces require a high injection pressure for a given injection rate. Depending on salinity, the increase in injection pressure due to capillary forces can be offset by the dissolution of CO₂ in formation water and its compressibility. Higher capillary forces also reduce gravity segregation, and this gives a more homogeneous CO₂ plume which improves the dissolution of CO₂. The solubility of CO₂ in formation water decreases with increasing salinity which requires an increased injection pressure. Higher salinity and capillary pressure can even block the pores, causing an increased salt precipitation. Simulations with various pressure-temperature conditions and modified salinity and capillary pressure curves demonstrate that, with the injection pressures similar for both cold and warm basins at a given injection rate, CO₂ dissolves about 10% more in the warm basin water than in the cold basin. The increase in dissolution lowers the injection pressure compensating the disadvantage of low CO₂ density and compressibility for storage in warm basins.     

Developing column material for the separation of serum amyloid P and C reactive protein from biological sources

In this study, we have investigated the isolation of serum amyloid P (SAP) and C‐reactive protein (CRP) from rainbow trout. It has recently been found that SAP is deposited in atherosclerotic lesions or neurofibrillary tangles, which are related to aging process and Alzheimer's disease. Given the importance of CRP, the CRP level in blood is becoming recognized as a potential means of monitoring cardiovascular risk. These two proteins, members of the pentraxin family of oligomeric serum proteins, were isolated from rainbow trout using N‐methacryloyl‐phosphoserine (MA‐pSer) immobilized poly (2‐hydroxy ethylmethacrylate) (PHEMA) cryogels as a column material in a fast protein liquid chromatography system. The separation process was verified in two steps. First, SAP and CRP proteins were isolated together from serum sample of rainbow trout using MA‐pSer/PHEMA cryogel columns. Second, SAP protein was separated chromatographically from CRP protein using the Ca²⁺ ion immobilized PHEMA cryogel column. According to the data, a new and effective technique has been developed for the isolation of SAP and CRP proteins from a biological source, rainbow trout. Finally, purified SAP and CRP were loaded using sodium dodecyl sulfate–polyacrylamide gel and western blot analysis to investigate the purity of chromatographically isolated SAP and CRP compared with commertial SAP and CRP. Copyright © 2014 John Wiley & Sons, Ltd.