Partial purification and some properties of a hemolymph lectin from panstrongylus megistus (Hemiptera,Reduviidae)

Hemagglutinating activity was studied in homogenates of three embryonic stages, and in the hemolymph of most instar larvae and in adult insects of Panstrongylus megistus, an important Chagas' disease vector in Brazil. A hemolymph lectin from the 5th instar larvae of P. megistus was purified through a biospecific adsorption by using formaldehyde-treated erythrocytes. The lectin fraction was desorbed with 0.2M D-galactose in 0.15M NaCl. The lectin fraction activity was inhibited by L-rhamnose, D-lactose, raffinose, D-galactose, and D-fucose. The electrophoretic pattern to native and acidic proteins resolved lectin fraction in two main bands with lectin activity. These bands were considered as multiple molecular forms or isoforms of P. megistus lectin. Under denaturating conditions, isoform 1 showed one band with apparent mol wt (MW) of 64 kDa while isoform 2 was resolved in two bands with MW of 64 and 33 kDa.     

Emission of neutrino pairs by an electron in an ultrastrong magnetic field

The transitions of relativistic electrons in a magnetic field with the emission of an antineutrino-neutrino pair are considered. Probabilities are obtained for electron transitions to the ground state and weakly excited states. It is shown that in the case of an ultrastrong magnetic field (HH_o=m²c³/e=4.41·10¹³ G) such transitions made a considerable contribution to the total probability of the process.Translated from Izvestiya Vysshikh Uchebnykh Zavedenii, Fizika, No. 3, pp. 110–114, March, 1978.In conclusion, the authors express their profound gratitude to A. A. Sokolov for his attention to our work.     

Doping of solid solutions in liquid epitaxy. III. Tellurium in AlxGa1−xSb

A calculation is made of the dependences of the coefficient of the tellurium distribution K_Te and the electron concentration n in Al_xGa_1–xSb on the composition of the solid solution and the growth temperature. It is shown that K_Te must decrease with increasing x. The dependence of K_Te on the temperature is also determined by x. Experimental results were obtained on Al_xGa_1–x Sb (0 x 0.74) films grown by liquid epitaxy. The electron concentration in the films was measured through the thermoelectric power and the capacitance-voltage characteristics of Schottky barriers. Satisfactory agreement with the results of the calculation was obtained.Translated from Izvestiya Vysshikh Uchebnykh Zavedenii, Fizika, No. 4, pp. 19–25, April, 1980.We thank G. K. Arbuzov for assistance in preparing the surface-barrier structures.     

Lanthanum chromites partially substituted by calcium,strontium and barium synthesized by urea combustion

Because of their electrical, magnetic and catalytic properties rare earth and transition metal mixed oxides are important compounds. Lanthanum chromites have been extensively used as solid oxide fuel cell (SOFC) interconnect materials. In this work, lanthanum chromites partially substituted by alkaline earth metals were synthesized by the urea combustion process. TG and DSC techniques were used to evaluate the presence of the organic material in the powder after reaction on the hot plate. The powders were calcinated at 900°C and characterized by XRD and SEM. The results show that the particles have nanometric dimensions and the perovskite structure was formed.     

To the theory of electron scattering by polyatomic molecules

Within the Born-Oppenheimer adiabatic perturbation theory, an equation was obtained for the intensity of fast electron scattering by polyatomic molecules. All of its parameters are explicitly defined by vibronic interaction in a molecule; due to this, quantum chemical calculations are fully applicable to electron diffraction studies of molecular geometries. Engels Anti-Aircraft Missile Higher Military School. Translated fromZhurmal Strukturnoi Khimii, Vol. 35, No. 2, pp. 40–45, March–April, 1994. Translated by L. Smolina     

MINDO/3 calculation of the atom—Atom self- and mutual polarizabilities for a hydrocarbon series

A MINDO/3 calculation based on the Hartree—Fock perturbation theory gave the atom—atom self- and mutual polarizabilities of a series of hydrocarbons. A study was carried out on the effect of the perturbation-induced distortion of the electron—electron interaction and molecular relaxation on the calculated values.Translated from Teoreticheskaya i Éksperimental'naya Khimiya, Vol. 31, No. 4, pp. 227–231, July–August, 1995.     

EXAFS study of spin transition effect on the spatial and electronic structure of Fe(II) complexes with triazoles

EXAFS spectroscopy is used to investigate the characteristic features of the spatial and electronic structure of the polynuclear Fe(II) complexes Fe(ATR)₃A₂ (where A is the NO ₃ ^? , BF ₄ ^? , Br^?, or ClO ₄ ^? anion and ATR is 4-amino-1,2,4-triazole) and their magnetically diluted phases Fe_xZn_1?x(ATR)₃(NO₃)₂. The absolute distances from Fe and Zn to the surrounding atoms are determined at temperatures higher and lower than the spin transition point. In all complexes, the spin transition is accompanied by significant changes in the local environment of Fe atoms, while in the magnetically diluted phases the surrounding of zinc remains unchanged. It is shown that addition of Zn atoms distorts the triazole rings in the low-spin state of the complexes. No localized anions were revealed within 3.3 Å from the Fe and Zn atoms. It is shown that a decrease in the spin transition temperature correlates with an increase in Fe?N distances in the low-spin complexes due to magnetic dilution and substitution of anions in the series NO ₃ ^? , BF ₄ ^? , Br^?, ClO ₄ ^? of ATR-containing complexes.     

Encapsulation of Mithramycin in Liposomes in Response to a Transmembrane Gradient of Calcium Ions

The recent discovery that mithramycin(MTR) in aqueous solution forms a high affinity[Ca(MTR)4]2- complex led us to the idea thatCa2+-loaded liposomes might be able to accumulateMTR in their aqueous internal compartment. Wetherefore investigated the uptake of MTR into largeunilamellar vesicles (LUV) containing NaCl orCaCl2. Our data show that MTR was efficientlyaccumulated within LUV made fromdipalmitoylphosphatidylcholine and cholesterol, onlywhen the liposomes contained Ca2+ and wereresuspended in a Ca2+-free medium. A drugencapsulation efficiency as high as 60% was achieved,at a drug to lipid molar ratio of 1/18. The circulardichroism and fluorescence excitation spectra ofliposome-encapsulated MTR (LMTR) displayed strongsimilarities with those of the [Ca(MTR)4]2-complex. LMTR was found to be stable, when submittedto conditions that destabilized the[Ca(MTR)4]2- complex. Upon dilution andincubation for 24 h at 37 °C, MTR-containingliposomes did not release a significant amount of MTR.These properties were attributed to the formation ofa high affinity complex between MTR and Ca2+inthe aqueous compartment of liposomes.     

N-oxides of 2- and 4-azafluorenones and nitro derivatives of 2- and 4-azafluorenes

It was demonstrated by PMR spectroscopy that mixtures of N-oxides of 6- and 7-nitro derivatives are formed in the nitration of the N-oxides of 3-methyl-2- and 4-azafluorenes. The products were deoxygenated to give nitro derivatives of 2- and 4-azafluorenes. The N-oxides of nitro-substituted azafluorenes were converted to salts of the aci forms by the action of alkali.Translated from Khimiya Geterotsiklicheskikh Soedinenii, No. 10, pp. 1396–1399, October, 1982.The authors thank K. F. Turchin for his assistance in recording the spectrum of nitration product VI at 200 MHz.