适配体磁纳米探针-激光诱导荧光特异识别微囊藻毒素-LR

微囊藻毒素-LR(MC-LR)具有强烈的肝毒性、致癌性和发育毒性,亟需严格监测。为实现环境水体中微痕量MC-LR的快速特异灵敏的分析识别,以金属有机框架化合物(MOF)为介导,增强磁纳米粒子比表面积,高效负载纳米金和适配体-cDNA分子杂交荧光探针,研发新型的适配体功能化磁纳米荧光探针(Fe₃O₄@MIL-101-NH₂@Au@aptamer),实现了对MC-LR的适配体特异识别-激光诱导荧光(LIF)超高灵敏分析。论文详细研究了适配体磁纳米探针荧光检测MC-LR的可行性、 MC-LR测定的优化条件及其方法性能。结果表明：MOF修饰的磁纳米颗粒Fe₃O₄@MIL-101-NH₂的Brunauer-Emmett-Teller(BET)比表面积达到114.02 m²·g^-1,比单一Fe₃O₄提高了近5倍,适配体-cDNA杂交荧光探针在磁纳米颗粒表面的修饰率达98%以上,适配...     

毛细管电泳紫外吸收法分离检测海洋生物样品中不同形态的砷化合物

本研究建立了对亚砷酸盐As(Ⅲ)、二甲基砷(DMA)、对甲基苯砷酸(p-As)、一甲基砷(MMA)、砷酸盐(As(Ⅴ)) 5种不同形态的砷化合物的毛细管区带电泳(CZE)分离紫外检测方法,研究了检测波长、缓冲体系种类、pH值及其浓度、分离电压、温度、进样时间等因素对5种形态砷化合物的分离度、灵敏度、重现性等的影响.结果表明,在25 ℃、195 nm、20 mmol/L NaH2PO4-5 mmol/L Na2B4O7(pH=6.25)缓冲溶液、20 kV运行电压、3.0 kPa压力进样10 s条件下,5种不同形态砷化合物在11 min内取得完全分离,5种不同形态砷化合物的迁移时间和峰面积的RSD为0.50%～1.51%和1.65%～3.36%,检出限(3S/N)为0.004～0.30 mg/L.本法成功地应用于虾米中不同砷形态含量的测定,其回收率在93%～106%之间.     

High performance liquid chromatography fingerprints of polysaccharide hy⁃ drolysate monosaccharides in Mesona chinensis BenthEI北大核心CSCD

：Based on the pre-column derivatization of monosaccharides with 1-phenyl-3-methyl-5-pyrazolone,the fingerprints of polysaccharide hydrolysate monosaccharides in the extract of Mesona chinensis Benth was established using reversed-phase high performance liquid chromatography （HPLC） gradient elution/UV detection technology,which was used for the analysis of the components and content of polysaccharide hydrolyzed monosaccharides in the aqueous extract of Mesona chinensis Benth,and eight monosaccharide components including ribose,rhamnose,glucuronic acid,galacturonic acid,glucose,galactose,xylose and arabinose were identified. Similarity analysis,cluster analysis,principal component analysis of the HPLC fingerprints of monosaccharide components were carried out,and the changes in the contents of galactose, galacturonic acid and glucose monosaccharide components in various samples were clarified. Regional differences lead to differences in cluster analysis,and there are certain differences in the content and distribution of monosaccharide components produced by polysaccharide hydrolysis in Mesona chinensis Benth collected from different origins. The method can provide a potential technology for characterizing monosaccharide components of the polysaccharide in aqueous extract of Mesona chinensis Benth. © 2023, Youke Publishing Co.,Ltd. All rights reserved.