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Nina Rembiakowska Vitalij Novickij Dagmara Baczyska Magda Dubiska-Magiera Jolanta Saczko Julia Rudno-Rudziska Magdalena Maciejewska Julita Kulbacka 《Molecules (Basel, Switzerland)》2022,27(7)
(1) Background: Pulsed electric field (PEF) techniques are commonly used to support the delivery of various molecules. A PEF seems a promising method for low permeability drugs or when cells demonstrate therapy resistance and the cell membrane becomes an impermeable barrier. (2) Methods: In this study, we have used doxorubicin-resistant and sensitive models of human breast cancer (MCF-7/DX, MCF-7/WT) and colon cancer cells (LoVo, LoVoDX). The study aimed to investigate the susceptibility of the cells to doxorubicin (DOX) and electric fields in the 20–900 ns pulse duration range. The viability assay was utilized to evaluate the PEF protocols’ efficacy. Cell confluency and reduced glutathione were measured after PEF protocols. (3) Results: The obtained results showed that PEFs significantly supported doxorubicin delivery and cytotoxicity after 48 and 72 h. The 60 kV/cm ultrashort pulses × 20 ns × 400 had the most significant cytotoxic anticancer effect. The increase in DOX concentration provokes a decrease in cell viability, affected cell confluency, and reduced GSSH when combined with the ESOPE (European Standard Operating Procedures of Electrochemotherapy) protocol. Additionally, reactive oxygen species after PEF and PEF-DOX were detected. (4) Conclusions: Ultrashort electric pulses with low DOX content or ESOPE with higher DOX content seem the most promising in colon and breast cancer treatment. 相似文献
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Julita K. Grzeskowiak Anne Tscheliessnig Poh Choo Toh Janet Chusainow Yih Yean Lee Niki Wong Alois Jungbauer 《Journal of chromatography. A》2009,1216(24):4902-4912
Although Staphylococcus Protein A (SpA) affinity chromatography is the state of the art capture step for antibody purification, non-affinity methods are more economical. We used two-dimensional fluorescence difference gel electrophoresis (2-D DIGE) to evaluate the purification of a recombinant IgG1 antibody from cultured cells, with two different processes: (1) SpA capture followed by cation-exchange chromatography (CEX); and (2) CEX capture, followed by anion exchanger, then hydrophobic interaction chromatography. Efficiencies were similar in sodium dodecylsulphate polyacrylamide gel electrophoresis and size-exclusion chromatography; however, 2-D DIGE revealed higher efficiency with SpA than with CEX capture. Thus, 2-D DIGE is a valuable tool for downstream process development. 相似文献
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Three new lacunar-type derivatives of β-unsubstituted dibenzotetraaza[14]annulene have been synthesized along with a range of their open-chain pendant group containing counterparts. The crystal structures of two representative products have been determined and the noncovalent interactions explored. A number of C-H?π and π?π interactions involving aliphatic chains and aromatic regions of the macrocycle have been evidenced. The relative ease and high yield of lacunization are suggested to be largely due to self-assembly processes, driven by favorable noncovalent interactions between reacting units. 相似文献
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Krzysztof Lewiski Julita Eilmes 《Journal of inclusion phenomena and macrocyclic chemistry》2005,52(3-4):261-266
Recrystallization of the title Cu(II) complex from pyridine afforded solid (1:1) pyridine inclusion compound. The crystal structure revealed a pleated-sheet-like arrangement of saddle-shaped molecules of the host, with molecules of pyridine enclathrated within intermolecular cavities. Careful analysis of intermolecular contacts showed that weak aromatic edge-to-face (C–H···Fπ) interactions occur between pyridine and host molecules. The crystal packing appeared to be also stabilized by the host–host C–H···O hydrogen bonds.in final form: 22 December 2004This revised version was published online in July 2005 with a corrected issue number. 相似文献
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The new α‐aminophosphonic acids are synthesized, reacting (9H‐fluoren‐9‐yl)urea with formaldehyde and phosphorus trichloride. (9H‐Fluoren‐9‐yl)urea was prepared from spiro(fluoren‐9,4′‐imidazolidine)‐2′,5′‐dione by alkaline hydrolysis with Ba(OH)2. The structure of the title compounds was proved by means of IR, 1H, 13C, and 31P NMR spectroscopy. © 2008 Wiley Periodicals, Inc. Heteroatom Chem 19:719–722, 2008; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/hc.20500 相似文献
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Beata Godlewska-ykiewicz Julita Malejko Piotr Haaburda Barbara Leniewska Anatol Kojo 《Microchemical Journal》2007,85(2):314-320
A novel flow-injection chemiluminescent (CL) method of determination of trace amounts of Pt(IV) based on its catalytic effect on the reaction of oxidation of luminol in alkaline solution is proposed. The method employs on-line separation of analyte on the column containing green algae Chlorella vulgaris immobilized on Cellex-T support. The application of biosorption process for elimination of interferences resulted in the increase in the tolerable matrix ions concentration by several orders of magnitude. The influence of dissolved organic matter on chemiluminescence of luminol was eliminated by means of a reversed phase C18 column. Under optimized conditions the suitable selectivity, good sensitivity, and low limit of detection (LOD = 0.057 ng mL− 1) of the method were obtained. The method was applied to the analysis of spiked river water samples. 相似文献
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Jan Romański Julita Jóźwik Christian Chapuis Janusz Jurczak 《Helvetica chimica acta》2007,90(11):2116-2131
Asymmetric 1,3‐dipolar cycloadditions of chiral derivatives of the nitrile oxides 3a – 3c derived from (2R)‐bornane‐10,2‐sultam, (2R)‐10‐(dicyclohexylsulfamoyl)isoborneol, and (1R)‐8‐phenylmenthol, to either (E)‐stilbene 4 or dimethyl fumarate 5 , leading to the corresponding 4,5‐dihydroisoxazoles 6a – 6c and 7a – 7c in both moderate yields and diastereoselectivities, are presented. All cycloadducts were converted into the corresponding methyl esters 8 and 9 , which were used for determination of their enantiomeric purities via chiral HPLC analyses. In the case of both stilbene cycloadducts 6a and 6b , their absolute configurations were determined by X‐ray crystal‐structure analyses. These [3+2] cycloadditions suggest the participation of the thermodynamically less stable SO2/CO syn‐conformer in the πy approach along the C?O bond of the linear nitrile oxide 3a . 相似文献
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Marta Molska Julita Regua Anna Grygier Agata Muzsik-Kazimierska Magdalena Rudziska Anna Gramza-Michaowska 《Molecules (Basel, Switzerland)》2022,27(14)
The aim of the study was to evaluate the effect of the addition of Fagopyrum esculentum Moench buckwheat sprouts modified with the addition of Saccharomyces cerevisiae var. boulardii to an atherogenic diet on the metabolism of sterols and fatty acids in rats. It was noticed in the study that the group fed with modified sprouts (HFDPRS) had a greater amount of sterols by 75.2%, compared to the group fed on an atherogenic diet (HFD). The content of cholesterol in the liver and feces was lower in the HFDPRS group than the HFD group. In the serum of the HFDPRS group, a more significant amount of the following acids was observed: C18:2 (increase by 13.5%), C20:4 (increase by 15.1%), and C22:6 (increase by 13.1%), compared to the HFDCS group. Regarding the biochemical parameters, it was noted that the group fed the diet with the addition of probiotic-rich sprouts diet had lower non-HDL, LDL-C and CRP ratios compared to the group fed the high-fat diet. The obtained results indicate that adding modified buckwheat sprouts to the diet by adding the probiotic strain of the yeast may have a significant impact on the metabolism of the indicated components in the organism. 相似文献
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Julita Mrowiec-Bialoń 《Thermochimica Acta》2006,443(1):49-52
Thermogravimetry was applied to determine the surface hydroxyls coverage in the mesostructured cellular foams (MCFs) calcined at different temperatures and then rehydroxylated by contacting with water vapor or liquid. The TG measurements were performed by heating MCFs in air stream using a three-step temperature program: (i) at rate of 5 °C min−1 from 25 to 200 °C; (ii) held at 200 °C for 30 min; and (iii) heating at rate of 10 °C min−1 up to 1100 °C. The hydroxyls content was calculated from weight loss during third step. The hydroxyls density appeared to depend strongly on the calcination temperature and the subsequent contact with water vapor. When MCFs were exposed for a short period (ca. 1 min) to moist air the hydroxyls content increased rapidly, more in the samples calcined at 300 °C than 500 °C, to attain surface densities of 4.75 and 1.6 OH nm−2, respectively. The 2-h contact with water vapor resulted in slower further increase of hydroxyls densities, to values of 5.45 and 2.9 OH nm−2, for samples calcined at 300 and 500 °C, but longer contacts had no significant effect. A similar trend was also observed when sample was treated with liquid water. 相似文献