UNSCHEDULED DNA SYNTHESIS: A QUANTITATIVE INDICATOR OF RESIDUAL IMMUNODETECTABLE PYRIMIDINE DIMERS IN HUMAN FIBROBLASTS AFTER ULTRAVIOLET-B IRRADIATION

We have addressed the question whether the level of UV-B induced DNA damage can be accurately assessed by the measurement of the rate of unscheduled DNA synthesis (UDS). Cultured human fibroblasts were irradiated with UV radiation at 290, 313 or 365 nm. The LD50 was 85 J/m2 at 290 nm, 4500 J/m2 at 313 nm, and 70 kJ/m2 at 365 nm. The analysis of UDS measurements indicate complete arrest of repair processes within 24 h after irradiation, irrespective of the dose (in the range 10-60 J/m2 at 290 nm, and 250-1000 J/m2 at 313 nm). Irradiation at 365 nm failed to yield detectable evidence of UDS. Incubation of irradiated cells with an antiserum directed against both 6-4 type and cyclobutane-type pyrimidine dimers shows a clear parallelism between the disappearance of the antibody-binding determinants and the variation of the rate of UDS vs time after the end of the irradiation. Thus it is concluded that in UV-B irradiated normal cultured human fibroblasts, the lack of UDS reflects the absence of immunodetectable pyrimidine dimers.     

Prediction of Turbulent Separated Flow in a Turnaround Duct Using Wall Functions and Adaptivity

This paper presents an application of adaptive remeshing to the prediction of turbulent separated flows. The paper shows that the κ - ε model with wall functions can predict separated flows along smooth curved surfaces. Success is achieved if the wall functions exhibit values of y⁺ close to 30, and if meshes are fine enough to guarantee that wall function boundary conditions are grid converged. Adaptive remeshing proves to be a very cost effective tool in this context. The methodology is demonstrated on a problem possessing a closed form solution to establish the performance and reliability of the proposed approach. The method is then applied to prediction of turbulent flow in an annular, axisymmetric turnaround duct (TAD). Predictions from two computational models of the TAD are compared with experimental measurements. The importance of appropriate meshes to achieve grid independent solutions is demonstrated in both cases. Better agreement with measurements is obtained when partially developed profiles of u, κ, and ε are specified at the TAD inlet.