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1.
Mikaru Ikedo Masanobu Mori Kazumasa Kurachi Wenzhi Hu Kazuhiko Tanaka 《Analytical sciences》2006,22(1):117-121
The selective and simultaneous ion-exclusion chromatography (IEC) with UV-detection on a weakly acidic cation-exchange resin column in the H+ -form (TSKgel Super IC-A/C) was developed and applied for the simultaneous determination of phosphate and silicate ions as the water quality parameters required for optimizing the water-leaching process for ceramics glaze raw materials of natural origin including feldspar, woods-ash, and straw-ash. Phosphate and silicate ions in these water-leaching process water samples were separated selectively from the coexisting anions such as sulfate, chloride, nitrate and carbonate ions, based on the ion-exclusion separation mechanism. They were detected selectively and simultaneously by a postcolumn derivatization with molybdenum-yellow using the UV-detector. Under the optimized separation and detection conditions (eluent, 0-1 mM sulfuric acid; reactant, 10 mM sodium molybdate-25 mM sulfuric acid; detector, UV at 370 nm; temperature, 45 degrees C), the linearity of calibration was in the range 0.1 - 10 ppm for both phosphate and silicate ions, and the detection limits at S/N = 3 were 2.58 ppb for silicate ions and 4.75 ppb for phosphate ions. The effectiveness of this method was demonstrated in practical applications to the water-leaching process for some ceramics glaze raw materials. 相似文献
2.
SSG is an antitumor branched (1----3)-beta-D-glucan obtained from the culture filtrate of Sclerotinia sclerotiorum IFO 9395. Hydroxyethylation of SSG higher than MS 0.45 (MS value represents molar ratio of hydroxyethyl group vs. glucosyl group) by ethyleneoxide in aqueous sodium hydroxide lose the antitumor activity. Degradation of branching point of hydroxyethylated SSG (HE-SSG) by the sequential treatments of periodate oxidation, borohydride reduction, and mild acid hydrolysis of these derivatives regenerated the antitumor activity. These results directly demonstrated that the branching point covered, at least a part of, the dormant active site of SSG. 相似文献
3.
Kobayashi Y Kumar GB Kurachi T Acharya HP Yamazaki T Kitazume T 《The Journal of organic chemistry》2001,66(6):2011-2018
By using the convenient protocol for conversion of 2-substituted furans into 4-oxo-2-alkenoic acids ((i) NBS, (ii) NaClO(2)), macrosphelide B (2) was synthesized from furyl alcohol 5 (>98% ee) and acid 6 (99% ee). The protocol was first applied to the PMB ether of 5 to afford acid 13b. On the other hand, DCC condensation of acid 6 with 5 gave 16 after deprotection of the TBS group. Condensation was again carried out between 13b and 16 to furnish the key ketone 17, which upon reduction with Zn(BH(4))(2) afforded anti alcohol 18 stereoselectively (15:1). After protection/deprotection steps, the furan 18 was converted to seco acid 3 by using the furan oxidation protocol mentioned above, and lactonization of 3 with Cl(3)C(6)H(2)COCl, Et(3)N, and DMAP afforded 22 (MOM ether of 2), which upon deprotection with TFA produced 2. Transformation of 22 to macrosphelide A (1) was then investigated. Although the chelation-controlled reduction of 22 should afford the desired anti alcohol 24, Zn(BH(4))(2) at <-90 degrees C gave a 2 approximately 1:1 mixture of anti/syn alcohols. On the contrary, reduction with NaBH(4) in MeOH at -15 degrees C produced the syn isomer 23 with >10:1 diastereoselectivity. Mitsunobu inversion of the resulting C(14)-hydroxyl group and deprotection of the MOM group with TFA afforded 1. Similarly, reduction of 2 with NaBH(4) afforded the C(14)-epimer of 1 stereoselectively. The observed stereoselectivity in the reductions of 22 and 2 could be explained on the basis of computer-assisted calculation, which showed presence of the low-energy conformers responsible for the stereoselective reduction. In addition, conversion of 2 to 1 was established, for the first time. 相似文献
4.
Narisawa Masaki Okabe Yoshio Iguchi Masahiro Okamura Kiyohito Kurachi Yasuo 《Journal of Sol-Gel Science and Technology》1998,12(3):143-152
Inorganic-organic hybrid gels were prepared by simultaneous condensation of the liquid mixture of ethyl silicate, ethyl borate and water soluble phenol resin with low molecular weight. The liquid mixtures were changed into lightly colored transparent gels after a catalyst addition and stirring. The obtained gels were crushed, dried and fired in controlled conditions to yield the inorganic precursors. Since the tailored inorganic precursors mainly consisted of SiO2 and carbon, silicon carbide was formed with the heat treatments in an Ar atmosphere beyond 1773 K with carbothermic reduction. The properties and morphology of the formed silicon carbide powders in terms of the starting precursor compositions and the conditions of the carbothermic reduction were investigated with SEM, XRD and TG-DTA analysis. 相似文献
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6.
C. F. Oliveira F. G. Basso E. C. Lins C. Kurachi J. Hebling V. S. Bagnato C. A. de Souza Costa 《Laser Physics》2010,20(7):1659-1666
Studies have shown that the increase of cell metabolism depends on the low level laser therapy (LLLT) parameters used to irradiate
the cells. However, the optimal laser dose to up-regulate pulp cell activity remains unknown. Consequently, the aim of this
study was to evaluate the metabolic response of odontoblast-like cells (MDPC-23) exposed to different LLLT doses. Cells at
20000 cells/cm2 were seeded in 24-well plates using plain culture medium (DMEM) and were incubated in a humidified incubator with 5% CO2 at 37°C. After 24 h, the culture medium was replaced by fresh DMEM supplemented with 5% (stress by nutritional deficit) or
10% fetal bovine serum (FBS). The cells were exposed to different laser doses from a near infrared diode laser prototype designed
to provide a uniform irradiation of the wells. The experimental groups were: G1: 1.5 J/cm2 + 5% FBS; G2: 1.5 J/cm2 + 10% FBS; G3: 5 J/cm2 + 5% FBS; G4: 5 J/cm2 + 10% FBS; G5: 19 J/cm2 + 5% FBS; G6: 19 J/cm2 + 10% FBS. LLLT was performed in 3 consecutive irradiation cycles with a 24-hour interval. Non-irradiated cells cultured
in DMEM supplemented with either 5 or 10% FBS served as control groups. The analysis of the metabolic response was performed
by the MTT assay 3 h after the last irradiation. G1 presented an increase in SDH enzyme activity and differed significantly
(Mann-Whitney test, p < 0.05) from the other groups. Analysis by scanning electron microscopy showed normal cell morphology in all groups. Under
the tested conditions, LLLT stimulated the metabolic activity of MDPC-23 cultured in DMEM supplemented with 5% FBS and exposed
to a laser dose of 1.5 J/cm2. These findings are relevant for further studies on the action of near infrared lasers on cells with odontoblast phenotype. 相似文献
7.
Dr. Nahyun Kwon Gabriel O. Jasinevicius Dr. Giulia Kassab Lili Ding Jiachuan Bu Letícia P. Martinelli Dr. Vinicius G. Ferreira Alexander Dhaliwal Dr. Harley H. L. Chan Yulin Mo Dr. Vanderlei S. Bagnato Prof. Dr. Cristina Kurachi Dr. Juan Chen Prof. Dr. Gang Zheng Prof. Dr. Hilde H. Buzzá 《Angewandte Chemie (International ed. in English)》2023,62(28):e202305564
Indocyanine green (ICG) is the only near-infrared (NIR) dye approved for clinical use. Despite its versatility in photonic applications and potential for photothermal therapy, its photobleaching hinders its application. Here we discovered a nanostructure of dimeric ICG (Nano-dICG) generated by using ICG to stabilize nanoemulsions, after which ICG enabled complete dimerization on the nanoemulsion shell, followed by J-aggregation of ICG-dimer, resulting in a narrow, red-shifted (780 nm→894 nm) and intense (≈2-fold) absorbance. Compared to ICG, Nano-dICG demonstrated superior photothermal conversion (2-fold higher), significantly reduced photodegradation (−9.6 % vs. −46.3 %), and undiminished photothermal effect (7 vs. 2 cycles) under repeated irradiations, in addition to excellent colloidal and structural stabilities. Following intravenous injection, Nano-dICG enabled real-time tracking of its delivery to mouse tumors within 24 h by photoacoustic imaging at NIR wavelength (890 nm) distinct from the endogenous signal to guide effective photothermal therapy. The unprecedented finding of nanostructure-driven ICG dimerization leads to an ultra-stable phototheranostic platform. 相似文献
8.
Ferraz RC Fontana CR Ribeiro AP Trindade FZ Bartoloni FH Baader JW Lins EC Bagnato VS Kurachi C 《Journal of photochemistry and photobiology. B, Biology》2011,103(2):87-92
The photodynamic therapy (PDT) is a combination of using a photosensitizer agent, light and oxygen that can cause oxidative cellular damage. This technique is applied in several cases, including for microbial control. The most extensively studied light sources for this purpose are lasers and LED-based systems. Few studies treat alternative light sources based PDT. Sources which present flexibility, portability and economic advantages are of great interest. In this study, we evaluated the in vitro feasibility for the use of chemiluminescence as a PDT light source to induce Staphylococcus aureus reduction. The Photogem? concentration varied from 0 to 75 μg/ml and the illumination time varied from 60 min to 240 min.The long exposure time was necessary due to the low irradiance achieved with chemiluminescence reaction at μW/cm2 level. The results demonstrated an effective microbial reduction of around 98% for the highest photosensitizer concentration and light dose. These data suggest the potential use of chemiluminescence as a light source for PDT microbial control, with advantages in terms of flexibility, when compared with conventional sources. 相似文献
9.
D. P. Jacomassi E. C. Lins A. N. S. Rastelli C. Kurachi V. S. Bagnato 《Laser Physics》2009,19(12):2230-2235
The aim of this study was to evaluate the shrinkage of a microhybrid dental composite resin photo-activated by one LED with
different power densities by means of speckle technique. The dental composite resin Filtek™ Z-250 (3M/ESPE) at color A2 was used for the samples preparation. Uncured composite was packed in a metallic mold and irradiated during 20 s from 100
to 1000 mW cm−2. For the photo-activation of the samples, it was used a LED prototype (Light Emission Diode) with wavelength centered at
470 nm and adjustable power density until 1 W cm−2. The speckle patterns obtained from the bottom composite surfaces were monitored using a CCD camera without lens. The speckle
field is recorded in a digital picture and stored by CCD camera as the carrier of information on the displacement of the tested
surface. The calculated values were obtained for each pair of adjacent patterns and the changes in speckle contrast as a function
of time were obtained from six repeated measurements. The speckle contrasts obtained from the bottom surface with 100 mW cm−1 were smaller than those than the other power densities. The higher power densities provided the higher shrinkage. 相似文献
10.
Kurachi M. Nakamura Y. 《IEEE transactions on plasma science. IEEE Nuclear and Plasma Sciences Society》1991,19(2):262-269
Previously measured drift velocities and longitudinal diffusion coefficients in SiH4-Ar mixtures were reanalyzed, and the new vibrational excitation cross sections for monosilane molecules were obtained. Not only were the new cross sections consistent with swarm parameters, but also the ratio of their magnitudes at the resonance peak agreed with earlier measurements of the electron beam experiment. Having known the vibrational excitation cross sections, the analysis of the primary ionization coefficient measured in SiH4-Kr mixtures led to a consistent inelastic cross section, which possibly corresponds to the total section for neutral dissociations for the molecule. It was confirmed that the derived set of the cross sections also gave good agreement with the electron swarm parameters measured in pure monosilane gas 相似文献