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Sunisa Boontom Kanokthorn Boonkitpatarakul Mongkol Sukwattanasinitt Nakorn Niamnont 《Tetrahedron》2017,73(17):2483-2487
A novel thiosemicarbazone fluorophore (3) was successfully synthesized in 3 steps via Sonogashira coupling and Knoevenagel condensation using baker's yeast (Saccharomyces cerevisiae) as a biocatalyst. Compound 3 contains triphenylacetylene, which acts as a fluorophore, and thiosemicarbazone, which acts as a copper probe. Compound 3 exhibited highly selective detection of Cu2+ ions based on photoinduced electron transfer (PET) in 10 mM HEPES buffer pH 7.4/propylene glycol (70% (v/v)). A linear relationship was observed for Cu2+ concentrations between 0.1 nM and 10 μM, and the detection limit of the method was 0.14 nM. Additionally, 3 was utilized to detect Cu2+ in wastewater with satisfactory results, which highlighted its potential for real sample applications. 相似文献
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