全文获取类型
收费全文 | 143篇 |
免费 | 7篇 |
国内免费 | 1篇 |
专业分类
化学 | 120篇 |
晶体学 | 2篇 |
力学 | 3篇 |
数学 | 15篇 |
物理学 | 11篇 |
出版年
2024年 | 1篇 |
2023年 | 7篇 |
2022年 | 8篇 |
2021年 | 15篇 |
2020年 | 5篇 |
2019年 | 5篇 |
2018年 | 3篇 |
2017年 | 4篇 |
2016年 | 5篇 |
2015年 | 9篇 |
2014年 | 1篇 |
2013年 | 10篇 |
2012年 | 8篇 |
2011年 | 18篇 |
2010年 | 3篇 |
2009年 | 6篇 |
2008年 | 12篇 |
2007年 | 3篇 |
2006年 | 8篇 |
2005年 | 8篇 |
2004年 | 2篇 |
2003年 | 5篇 |
1998年 | 1篇 |
1995年 | 1篇 |
1993年 | 1篇 |
1989年 | 1篇 |
1988年 | 1篇 |
排序方式: 共有151条查询结果,搜索用时 15 毫秒
1.
2.
Yue GE Roper MG Balchunas C Pulsipher A Coon JJ Shabanowitz J Hunt DF Landers JP Ferrance JP 《Analytica chimica acta》2006,564(1):116-122
This work describes an integrated glass microdevice for proteomics, which directly couples proteolysis with affinity selection. Initial results with standard phosphopeptide fragments from β-casein in peptide mixtures showed selective capture of the phosphorylated fragments using immobilized metal affinity chromatography (IMAC) beads packed into a microchannel. Complete selectivity was seen with angiotensin, with capture of only the phosphorylated form. On-chip proteolysis, using immobilized trypsin beads packed into a separate channel, was directly coupled to the phosphopeptide capture and the integrated devices evaluated using β-casein. Captured and eluted fragments were analyzed using both capillary electrophoresis (CE) and capillary liquid chromatography/mass spectrometry (cLC/MS). The results show selective capture of only phosphopeptide fragments, but incomplete digestion of the protein was apparent from multiple peaks in the CE separations. The MS analysis indicated a capture bias on the IMAC column for the tetraphosphorylated peptide fragment over the monophosphorylated fragment. Application to digestion and capture of a serum fraction showed capture of material; however, non-specific binding was evident. Additional work will be required to fully optimize this system, but this work represents a novel sample preparation method, incorporating protein digestion on-line with affinity capture for proteomic applications. 相似文献
3.
4.
5.
Jiawang Zhou Ljiljana Stojanovi Andrey A. Berezin Tommaso Battisti Abigail Gill Benson M. Kariuki Davide Bonifazi Rachel Crespo-Otero Michael R. Wasielewski Yi-Lin Wu 《Chemical science》2021,12(2):767
Development of purely organic materials displaying room-temperature phosphorescence (RTP) will expand the toolbox of inorganic phosphors for imaging, sensing or display applications. While molecular solids were found to suppress non-radiative energy dissipation and make the RTP process kinetically favourable, such an effect should be enhanced by the presence of multivalent directional non-covalent interactions. Here we report phosphorescence of a series of fast triplet-forming tetraethyl naphthalene-1,4,5,8-tetracarboxylates. Various numbers of bromo substituents were introduced to modulate intermolecular halogen-bonding interactions. Bright RTP with quantum yields up to 20% was observed when the molecule is surrounded by a Br⋯O halogen-bonded network. Spectroscopic and computational analyses revealed that judicious heavy-atom positioning suppresses non-radiative relaxation and enhances intersystem crossing at the same time. The latter effect was found to be facilitated by the orbital angular momentum change, in addition to the conventional heavy-atom effect. Our results suggest the potential of multivalent non-covalent interactions for excited-state conformation and electronic control.The number and position of halogen substituents in purely organic π–π* chromophores critically affect the efficiency of phosphorescence. 相似文献
6.
Abigail O. Delawder Anusree Natraj Nathan D. Colley Tiana Saak Angelique F. Greene 《Supramolecular chemistry》2019,31(8):523-531
ABSTRACTAlthough it is well known that viologen radical cations can self-assemble into stacks or complexes on account of radical-radical pairing interactions, it has only recently been demonstrated that reduction of main-chain polyviologens integrated into hydrogel networks can trigger actuation. In these earlier examples, hydrogels comprising oligoethylene glycol-based polyviologens and poly(ethylene glycol) were functionalized with terminal azide groups to prepare ‘click’-based gels. Here, we report a new structural design for the functional polyviologen that consists of main-chain viologen subunits separated by hexamethylene groups instead of glycols and is capped at each end with styrene groups. Activation of this viologen-based macrocrosslinker was achieved using chemical- and photoreduction methods and its ability to undergo intramolecular chain-folding was monitored by absorption spectroscopy. Acrylate-based organogels and hydrogels were also prepared and a comparison was carried out to assess the actuator performance in each gel in terms of the rate of contraction and changes in stiffness. 相似文献
7.
N-(β-Hydroxy)amides can be cyclised by reaction with diisopropylcarbodiimide (DIC) to give the corresponding 2-oxazolines in high yields. The reaction requires only very mild Lewis-acid catalysis (5 mol % Cu(OTf)2) and can be accomplished with simple heating, or in very short reaction times under microwave irradiation. 相似文献
8.
Daniel R. Zuidema Sarah L. Williams Katherine J. Wert Karin J. Bosma Abigail L. Smith Robert C. Mebane 《合成通讯》2013,43(19):2927-2931
Aryl ketones are readily deoxygenated to their corresponding aryl alkanes upon treatment with Raney nickel catalyst in boiling 2-propanol.
9.
The coupling of rhodium carbenoids from vinyl diazoacetates with 2-thio-3-alkyl indoles was found to generate C(3) quaternary substituted indolines via a thionium ylide-initiated [3,3]-sigmatropic rearrangement. 相似文献
10.
Unknotting Tunnels and Seifert Surfaces 总被引:2,自引:0,他引:2
Let K be a knot with an unknotting tunnel and suppose thatK is not a 2-bridge knot. There is an invariant = p/q Q/2Z,with p odd, defined for the pair (K, ). The invariant has interesting geometric properties. It is oftenstraightforward to calculate; for example, for K a torus knotand an annulus-spanning arc, (K, ) = 1. Although is definedabstractly, it is naturally revealed when K is put in thinposition. If 1 then there is a minimal-genus Seifert surfaceF for K such that the tunnel can be slid and isotoped to lieon F. One consequence is that if (K, ) 1 then K > 1. Thisconfirms a conjecture of Goda and Teragaito for pairs (K, )with (K, ) 1. 2000 Mathematics Subject Classification 57M25,57M27. 相似文献