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猪、牛胰岛素经与MSC·ONSu选择性反应.随后Edman降解,和与MSC·Trp·ONp缩合,去保护制备了二个种属的[Trp]B1胰岛素类似物. 经醋酸纤维素电泳.氨基酸组成分析,UV吸收光谱和N-端分析鉴定,确定它们是均一的[Trp]B1胰岛素.鼠惊厥分析显示猪、牛[Trp]B1胰岛素的生物活力分别为18国际单位/毫克和17国际单位/毫克.分别相当于天然胰岛素的70%. 相似文献
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The rhombohedral crystal structure of [Trp]~(B1)-insulin has been refined, using data to 2 and atomic coordinates of 2-zinc porcine insulin as starting model, and through the use of the restrained least-squares method, to an R value of 0.24. The result of refinement shows that in comparison with the 2-zinc insulin structure, some changes in local conformation occur, and are asymmetric for the two independent molecules related by a local two-fold axis. The obvious conformational changes are found to be in molecule 1, at its B-chain N-terminus and A13-residue region. 相似文献
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本文报道了眼镜王蛇毒中LAO的纯化及某些性质,从眼镜王蛇毒中所分离的LAO在比活力、酶学性质及同工酶谱方面与从其他蛇毒来源的LAO存在显著不同. 聚丙烯酰胺凝胶电泳显示该酶是均一的.由梯度聚丙烯酰胺凝胶电泳测得全酶分子量约为1.5×105.SDS梯度聚丙烯酰胺凝胶测得亚基分子量为7.3×104.表明该酶是由二个分子量相同的亚基组成.吸收光谱显示每个分子含二个FAD. 酶氧化L-丙氨酸的最适pH为8.7—9.0.当底物浓度大于3n M时,出现明显产物抑制.该酶具有一定热稳定性,在55℃以下加热40分钟对酶活力基本无影响,当酶的浓度低于5.7微克/毫升时酶的浓度和酶反应速率呈线性关系. 相似文献
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This paper reports the isolation and some properties of L-amino acid oxidase from Ophi-ophagus hannah venom. The differences between L-amino acid oxidase from Ophiophagusohannah and that from other sources in specific activity, properties and the spectrum of iso-zymes are noticeable. The result of electrophoresis on polyacrylamide gel shows that this purified enzyme ishomogenous. The molecular weight determined by gradient polyacrylamide gel slab (4--30%)is around 15×10~4 dalton. The molecular weight of the subunit determined by SDS gradient gelelectrephoresis (4--30%) is around 7.3×10~4 dalton. Therefore, this enzyme is composed of twosubunits. The absorption spectrum reveals that there are two FADs in each molecule. The optimum pH of enzymic reaction is around 8.7--9.0 when L-leucine is used as substrate.The inhibition of the preducts is noticeable when substrate concentration goes beyond 3mM.This enzyme is heat stable and its activity would not decrease obviously after heating at 55℃for 40 min. A linear relationship between enzyme concentration and reaction rate was noticedwhen enzyme concentration was below 5.7μg/ml. 相似文献
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