Phytochemical Profile and Anticancer Potential of Endophytic Microorganisms from Liverwort Species,Marchantia polymorpha L.

Liverwort endophytes could be a source of new biologically active substances, especially when these spore-forming plants are known to produce compounds that are not found in other living organisms. Despite the significant development of plant endophytes research, there are only a few studies describing liverwort endophytic microorganisms and their metabolites. In the presented study, the analysis of the volatile compounds obtained from thallose liverwort species, Marchantia polymorpha L., and its endophytes was carried out. For this purpose, non-polar extracts of plant material and symbiotic microorganisms were obtained. The extracts were analyzed using gas chromatography coupled to mass spectrometry. Compounds with the structure of diketopiperazine in the endophyte extract were identified. Liverwort volatile extract was a rich source of cuparane-, chamigrane-, acorane-, and thujopsane-type sesquiterpenoids. The cytotoxicity of ethyl acetate extracts from endophytic microorganisms was evaluated on a panel of cancer (FaDu, HeLa, and SCC-25) cell lines and normal (VERO), and revealed significant anticancer potential towards hypopharyngeal squamous cell carcinoma and cervical adenocarcinoma.     

Three New Bibenzyl Derivatives from the Chinese Liverwort Marchantia polymorpha L.

From the Et₂O extract of the Chinese liverwort Marchantia polymorpha L., three new bibenzyl (=1,1′‐(ethane‐1,2‐diyl)bisbenzene) derivatives, compounds 1 – 3 , were obtained, together with the two known compounds marchantin J and perrottetin E. Their structures were established by NMR and HR‐EI‐MS analyses. Polymorphatin A ( 1 ) represents a new type of bis[bibenzyl] skeleton, and compound 3 is the first example of a bibenzyl oxidatively coupled to a phenylmethanol.