Determination of Serum Iron and Iron Binding Capacity Using 4-(2-Pyridylazo) Resorcinol (Par)

Abstract

Procedures are described for the determination of serum iron and iron binding capacity using the chromogenic reagent 4-(2-pyridylazo) resorcinol (PAR). The high molar absorptivity of the iron (II) PAR complex makes possible an approximate two fold increase in sensitivity over previous methods. The described procedures have few interferences, and give linear results for samples containing 0 – 750 μg/dl of iron.     

铁(Ⅲ)-4-(2-吡啶偶氮)间苯二酚络合物光化学还原反应的研究

在弱酸性溶液中,Fe3 -PAR络合物可发生光化学还原反应生成Fe2 -PAR络合物。研究了溶液酸度、光强度、有机酸、PAR溶液浓度对Fe3 -PAR络合物光化学还原反应的影响,并对反应机理作了初步探讨。在pH4.5HAc-NaAc缓冲溶液中,PAR浓度为1.8×10?4mol/L,125W高压汞灯照射15min,Fe3 -PAR络合物的光化学还原反应趋于完全。Fe2 浓度在0.12～3.2mg/L范围内符合比尔定律;检出限为0.025mg/L。     

Spektrophotometrische Untersuchung Der Wechselwirkung Zwischen Rh(III) und 4-(2-Pyridylazo)-Resorzin

Abstract

A detailed investigation has been made of Che pH versus light absorption curves of solutions of PAR and Rh(III) in various concentrations. It has been shown, that depending on tile acidity of the solution, two different substances will be formed; the optimal conditions of the formation of each substance has been found. The molar absorptivities have been determined and the equilibrium and stability constants of the formed substances have been computed from the absorbance versus pH curves.     

锰与4-(2-噻唑偶氮)间苯二酚络合吸附波的研究及应用

本文研究了在0.01mol/L NaOH—0.32％乙二胺—0.05mol/L酒石酸钾钠介质中,锰与4-(2-噻唑偶氮)间苯二酚(TAR)络合吸附波的极谱行为。当介质中含有0.003％TAR时,Mn会产生灵敏的导数极谱波,其峰电位为-0.75V(相对银汞齐电极,下同),方法检出限为1.5×10~(-9)mol/L,线性范围为3.6×1.0~(-9)mol/L～2.7×10~(-6)mol/L。研究了极谱波的性质、机理以及络合物的组成。方法成功地应用于某些植物叶中微量锰的含量测定。     

钯的PAR-氯化四苯鉮的萃取光度法测定

本文报道了钯与4-(2-吡啶偶氮)间苯二酚(PAR=H_2R,下同)和四苯钟离子形成离子缔合物的化学条件,经氯仿萃取后,建立了一种测定微量钯的萃取光度法。萃取的最佳pH为4.4,络合物的化学式为[(C_6H_5)_4As~+,PdClR~-],其最大吸收峰波长为532nm,表观摩尔吸光系数e_(532)=3.3×10~4L·mol~(-1)·cm~(-1),10mL萃取液中钯量在0～25μg内遵守比耳定律。方法简便,选择性好,用于催化剂中钯的测定,结果满意。     

钴与5－Cl－PADAB反应机理及其应用的研究

对钴与４－［（５－氯－２－吡啶）偶氮］－１，３－二氨基苯（５－Ｃｌ－ＰＡＤＡＢ）形成络合物的吸收光谱进行探讨，结果表明：试剂及络合物在不同酸度条件下形成不同型体。在酸性条件下，络合物为大阳离子，钴离子以三价形式存在，钻与试剂的络合比为１：２。在强酸性介质中，将此络合物用于光度测定钻，方法选择性及稳定性好，且灵敏度极高。对水、粮食、土壤、茶叶及维生素Ｂ＿（１２）中微量钻进行直接测定，获得满意结果。     

氯化钯与4-(2-吡啶偶氮)间苯二酚、硫酸四苯锑形成的离子缔合物的结构与性质研究

本文对离子缔合物[(C_6H_5)_4Sb~+,PdCl(C_(11)H_7N_3O_2)~-]进行了制备,经元素分析、红外光谱和核磁共振谱分析,确定了络合物的组成和结构,并测定了其吸收光谱、电导率和稳定常数。考察了络合物在水中的存在状态及其电荷性质,并对其形成机理进行了初探。     

新试剂2-(5-硝基-2-吡啶偶氮)-5-二甲氨基苯甲酸分光光度法测定微量钴

本文报道用新试剂2-(5-硝基-2-吡啶偶氮)-5-二甲氨基苯甲酸分光光度法测定微量钴。配合物的表观摩尔吸光系数为1.31×10~5L·mol~(-1)·cm~(-1),Co∶5-NO_2-PAMB=1∶2,Co浓度在0～14μg/25mL范围内服从比耳定律。用于金属有机化合物中微量钴的测定,结果与理论值相符。     

Determination of Lead Using a New Chromogenic Reagent 2-(2-Sulfo-4-acetylphenylazo)-7-(2,4,6-trichlorophenylazo)-1,8-dihydroxynaphthalene-3,6-disulfonic Acid

A novel chromogenic reagent, 2-(2-sulfo-4-acetylphenylazo)-7-(2,4,6-trichlorophenylazo)-1,8-dihydroxynaphthalene-3,6-disulfonic acid 1, was prepared by diazo coupling of 4-acetylaniline-2-sulfonic acid and 2,4,6-trichloroaniline to chromotropic acid through –N=N– groups. Based on this reagent, a simple, sensitive and selective spectrophotometric method was developed for the determination of lead. In 0.20M phosphoric acid medium, lead reacts with 1 to form a 1:2 blue complex with an absorption maximum of 654nm. Beers law is obeyed in the range of 0–0.6mgL^–1 of lead. The apparent molar absorptivity is 1.25×10⁵Lmol^–1cm^–1. The detection limit and quantification limit were found to be 0.63µgL^–1 and 2.1µgL^–1, respectively. The relative standard deviation for eleven replicate measurements was of 2.6%. The interference of foreign ions was also investigated. All the other foreign ions studied did not interfere with lead determination except for Ca(II) and Ba(II). The interference caused by Ca(II) and Ba(II) can be eliminated by prior extraction of lead with potassium iodide-methylisobutylketone (KI-MIBK). The proposed method was applied to the determination of lead in certified samples with satisfactory results.     

Color Reaction Between 4-(2-Pyridylazo)Resorcinol and Mercury (II) in the Presence of Surfactant,and Improved Spectrophotometric Determinations of Mercury(II) and Cyanide Ion with its Coloring

Abstract

The reactions among 4-(2-pyridylazo)resorcinol (PAR), mercury(II) and/or cyanide ion in the presence of water soluble surfactants alone or combination were systematically investigated at about pH 9. The spectrophotometric determinations of mercury(II) and cyanide ion were investigated by using the PAR-mercury(II)-HPC complex (3:2:2 molar ratio) in the presence of N-hexadecylpyridinium chloride (HPC) alone; calibration graphs were rectilinear in the ranges of 0 – 40 μg mercury(II) and 0 –10 μg cyanide ion in a final 10 ml with the apparent molar absorptivities of 5.9 × 10⁴ for mercury(II) and 2.5 × 10⁴ 1 mol^?1 cm^?1 for cyanide ion at 590 nm. The proposed method had advantages—rapidity, simplicity without solvent extraction, and sensitivity in comparison with reported solvent extraction methods. The interference of foreign ions decreased 1/2–l//4-fold compared with that in the presence of non-ionic surfactant alone.     

Spectrophotometic Determination Of Tin In Steels With2-(5-Nltro-2-Pyridylazo)-5-[N-n-Propyl-N-(3-Sulfopropyl)Amino]phenol

ABSTRACT

A highly sensitive azo dye, 2-(5-nitro-2-pyridylazo)-5-[N-n-propyl-N-(3-sulfopropyl)amino]phenol (Nitro-PAPS), is used as a colorimetric reagent for the determination of tin(IV) content. Nitro-PAPS reacts with tin(IV) to form a water-soluble complex in 1.0 M acetic acid. Full color development is attained within 5 minutes, and maintains constant absorbance for at least 24 hours. The apparent molar absorptivity is 7.7 x 10⁴ dm³ mol^?1 cm^?1 at a maximum wavelength of 580 ran. Beer's law is obeyed for tin(IV) in the range of 0-1.2 μg ml^?1. The proposed method is successfully applied to the determination of trace amounts of tin in steels.     

阳离子型微乳液对显色剂1—（2—吡啶偶氮）—2—萘酚的增溶作用

以光度法研究了阳离子型微乳液对显色剂１－（２－吡啶偶氮）－２－萘酚（ＰＡＮ）的增溶作用，通过测定ＰＡＮ在阳离子型ＣＴＭＡＢ／ｎ－Ｃ５Ｈ１１ＯＨ／ｎ－Ｃ９Ｈ２０微乳液中的“胶束位”ｎ和结合常数Ｋ，并与相同表面活性剂胶束体系比较，探讨了微乳液对显色剂ＰＡＮ的增溶作用。     

2-(5-溴-2-吡啶偶氮)-5-二乙氨基苯酚与二氯化双(丁氧羰乙基)锡的显色反应及其应用

在pH 2 .7的HAc NaAc缓冲溶液中和有TritonX 1 0 0存在下 ,二氯化双 (丁氧羰乙基 )锡 (简写为R2 SnCl2 )与 2 (5 溴 2 吡啶偶氮 ) 5 二乙氨基苯酚 (5 Br PADAP)形成 1∶1紫红色络合物 ,络合物的最大吸收波长为 5 0 5nm ;表观摩尔吸光系数 1 0 5× 1 0 5L·mol- 1 ·cm- 1 。R2 SnCl2 中的Sn浓度在 0 .0 6～ 1 .3 0mg L范围内符合比尔定律。方法用于PVC中间产品及污水中R2 SnCl2 的测定 ,结果满意。相对标准偏差分别为 2 4%和 4 5 % ;回收率在 95 4%～ 98 2 %之间。     

微乳液的增敏作用研究──Ni（Ⅱ）－5－Br－PADAP的分光光度法测定

以含水量８０％的阴离子型ＳＤＳ／ｎ－Ｃ＿（５）Ｈ＿（１１）ＯＨ／ｎ－Ｃ＿（７）Ｈ＿１６／Ｈ＿（２）Ｏ微乳液为介质，进行了Ｎｉ（Ⅱ）－５－Ｂｒ－ＰＡＤＡＰ的分光光度研究，其表观摩尔吸光系数为１．１３×１０￣５Ｌ·ｍｏｌ￣（－１）·ｃｍ￣（－１），与相应含水量的ＳＤＳ胶束介质比较，测定的灵敏度显著提高（后者为７．５×１０￣４Ｌ·ｍｏｌ￣（－１）·ｃｍ￣（－１）），样品分析结果令人满意。     

微乳液1-(2-吡啶偶氮)-2-萘酚分光光度法测定蔬菜样品中的钴

以十二烷基硫酸钠-溴代十六烷基三甲胺(SDS-CIMAB)复合微乳液为介质,对Co(Ⅱ)-1-(2-吡啶偶氮)-2-萘酚的显色反应条件进行了研究.结果表明该体系的λ_(max)=580nm,ε_(580)=3.4×10~4L·mol·cm~(-1),Co(Ⅱ)测定的线性范围为0～2.40mg/L.回收率在96%～103%之间,该法选择性好,用于蔬菜中Co(Ⅱ)含量的测定,得到了满意的结果.     

钒-2-(3,5-二溴-2-吡啶偶氮)-5-二乙氨基酚-二苯胍-NaBrO_3体系极谱催化波的研究

本文报道了钒-3,5-Br_2-PADAP-DPG-NaBrO_3新极谱催化波体系,确定了测定钒的适宜底液条件,测定钒的线性范围为0.04～18ng/ml,探讨了极谱波性质,本法成功地应用于自来水和矿样中钒的测定。     

稀土—4—（2—吡啶偶氮）间苯二酚螯合物的高效液相色谱分离和测定

本文讨论了反相离子对液相色谱法分离和检测稀土-4-(2-吡啶偶氮)间苯二酚螯合物的各种条件,在12 min内完成了9种稀土螫合物的分离,校正曲线的线性范围是(ppm):La(0.01～3.20),Ce(0.03～2.8),Pr(0.06～3.5),Nd(0.05～3.8),Sm(0.01～3.0),Eu(0.05～3.4),Tb(0.02～3.0),Ho(0.01～3.4),Er(0.06～4.0).变异系数小于1.2％.     

反相HPLC测定钒、铌、钽的2-(2-吡啶偶氮)-5-二乙氨基苯酚配合物

以2-(2-吡啶偶氮)-5-二乙氨基苯酚(PADAP)为柱前衍生试剂,在含0.1%酒石酸的10mmo1/L(pH3.5)HAc-NaAc缓冲溶液的甲醇/水(50:50,V/V)中(580nm检测),在C₁₈柱上于11min内实现了V、Nb、Ta的同时分离及测定,检出限(S/N=3)为0.34、0.29、7.30ng/mL.该法灵敏度高,用于矿样分析所得结果与推荐值相符,标准加入回收率为99.0%～101.1%.     

2-(5-溴-2-吡啶偶氮)-5-二乙氨基酚-BrO_3~--SCN-三元离子缔合物测定微量BrO_3~-的研究

本文研究了5-Br-PADAP在酸性介质中质子化,与BrO_3~-和SCN~-形成三元离子缔合物的最佳条件,其表观摩尔吸光系数ε_(540)=4.3×10~4L·mol~(-1)·cm~(-1),缔合物组成比为:5-Br-PADAP:BrO_(3-)SCN~-=1:1·1。提供了测定微量BrO_(3-)的新方法,可应用于化学试剂中微量BrO~(3-)的测定。此外,本文还探讨了反应机理。