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本文报道了以2-(6-甲基-2-苯并噻唑偶氮)-5-二乙氨基酚(MBTAE)作柱前衍生化试剂,在SpherisorbC_8柱上,以甲醇:水=80:20(V/V),含2mmol/LLi_2SO_4和10mmol/LpH5.0醋酸盐缓冲溶液作流动相,反相高效液相色谱分离和测定了Fe ̄(2+)、Co ̄(2+)、Ni ̄(2+)和Cu ̄(2+)。各金属离子的检出限(S/N=3)分别为(pg):Fe ̄(2+)6.40,Co ̄(2-)1.38,Ni ̄(2+)4.47,Cu ̄(2+)7.28。方法用于欧洲标准局,黑麦草标样的分析,所得结果与标准值相符。 相似文献
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喹啉基氮杂大环铜、镍配合物的结构和性质差异安道利,卜显和,李金亮,陈荣悌(南开大学化学系,天津300071)二氧四胺大环配体是Cu ̄(2+)、Ni ̄(2+)、Co ̄(2+)、Pt ̄(2+)等金属离子的优良配体,它们同时具有饱和大环多胺和寡聚肽的结构特... 相似文献
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离子色谱法快速同时测定营养药物中微量铜、锰、锌 总被引:12,自引:0,他引:12
在HPLC-CS5阳离子分离柱上选用5.0×10 ̄(-2)mol/L草酸(pN为5.24)作淋洗液分离了微量Cu ̄2+、Mn ̄2+和Zn ̄2+离子,用4-(2-吡啶偶氮基)间苯二酚作显色剂在520nm处连续检测,方法快速、简便、准确。本法用于施尔康片中微量Cu ̄2+、Mn ̄2+和Zn ̄2+离子的测定,结果令人满意。 相似文献
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配位聚合物PAAm-CuCl_2和cuCl_2·2H_2O的Cu ̄(2+)2pXPS谱上都有Shake-up峰,其与各自的Cu ̄(2+)2p3/2肩峰或主峰距离ΔEs2或ΔEs分别为8.7和8.6eV;但配位聚合物Cu(Ⅱ)-PVA却无此峰.由此推定,前者的Cu ̄(2+)是以sp ̄3杂化轨道接受PAAm4个链节单元C-N上N的孤对电子部分转移形成配位键;而Cu(Ⅱ)-PVA配位聚合物中的Cu ̄(2+)却以dsp ̄2杂化轨道接受PVA4个链节单元侧基O的孤对电子部分转移,产生配位键,即Cu ̄(2+)与PAAm或PVA的链节单元配位比均是1:4,此结果与电导率法测定PAAm-CuCl_2的配位比相同. 相似文献
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过渡元素三取代钨锗杂多酸盐异构体的氧化还原性质 总被引:1,自引:0,他引:1
用电化学方法研究了过渡元素三取代钨锗杂多酸盐M'_xH_y[GeW_9M_3(H_2O)_3O_(37)]·nH_2O(M'=Bu_4N ̄+,K ̄+;M=Cr ̄3+,Mn ̄2+,Co ̄2+,Ni ̄2+,Cu ̄2+)的α-,β-异构体在水溶液中的氧化还原质,提出了它们的还原机理,讨论了取代的过渡元素和杂多阴离子的结构对氧化还原性质的影响,比较了两种异构体的氧化能力。 相似文献
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Rare-earth ammonium sulfate octahydrates of R2(SO4)3·(NH4)2SO4·8H2O (R=Pr, Nd, Sm, and Eu) were synthesized by a wet process, and the stable temperature region for the anhydrous R2(SO4)3·(NH4)2SO4 form was clarified by thermogravimetry/differential thermal analysis, infrared, Raman, and electrical conductivity measurements. Detailed characterization of these double salts demonstrated that the thermal stability of anhydrous R2(SO4)3·(NH4)2SO4 is different between the Pr, Nd salts and the Sm, Eu salts, and the thermal decomposition behavior of these salts was quite different from the previous reports. 相似文献
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Christiane Auray-Blais Pamela Lavoie Shunji Tomatsu Vassili Valayannopoulos John J. Mitchell Julian Raiman Maxime Beaudoin Bruno Maranda Joe T.R. Clarke 《Analytica chimica acta》2016
Mucopolysaccharidoses (MPSs) are a group of disorders resulting from primary defects in lysosomal enzymes involved in the degradation of glycosaminoglycans (GAGs). Depending on the specific enzyme defect, the catabolism of one or more GAGs is blocked leading to accumulation in tissues and biological fluids. GAG measurements are important for high-risk screening, diagnosis, monitoring treatment efficacy, and patient follow up. The dimethylmethylene blue (DMB) spectrophotometric method commonly used in most biochemical genetics laboratories relies on a non-specific total GAG analysis which has led to false positive results, and even false negative results (mainly for MPS III and IV patients). The main objective of our project was to devise and validate a reliable tandem mass spectrometry multiplex analysis for the urine quantitation of four GAGs (dermatan sulfate (DS), heparan sulfate (HS), keratan sulfate (KS), and chondroitin sulfate (CS)) for an eventual technological transfer to the clinic. The developed methodology is rapid (7 min) and our results showed good intraday and interday precision (RSDs ≤ 8.7%) and accuracy (Biases range: −12.0%–18.4%). Linearity was good (r2 > 0.995) for DS, HS, CS, and KS calibration curves. In comparison with the DMB spectrophotometric method, this multiplex tandem mass spectrometry method allows GAG fractionation, thus a differentiation of MPS types, except for MPS I and II which are characterized by the same GAG profile. The devised method is a useful and reliable tool for diagnosis of MPS patients, as well as their monitoring and follow up, as shown by longitudinal studies. 相似文献
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Joseph A. Rard 《Journal of solution chemistry》1988,17(6):499-517
The aqueous solubilities of finely divided Pr2(SO4)3·8H2O(cr), Eu2(SO4)3·8H2O(cr), and Lu2(SO4)3·8H2O(cr) have been measured as a function of time at 25°C using isothermal saturation. Solubilities of the latter two salts showed a steady decrease with time, whereas Pr2(SO4)3·8H2O(cr) showed no such variation within the accuracy of the determinations. The turbidities of these filtered saturated solutions also decreased with time, and indicate that some colloidal rare earth sulfates were present. These colloidal particles (<0.2 m) have a large surface area, which contributes to the Gibbs energy of the solid phase, thus giving rise to enhanced solubilities. The micro-particles also grow with time, thereby reducing the surface area contribution to the Gibbs energy and also leaving fewer particles to pass through the filters. Extrapolation of solubilities to infinite time gives the solubilities of macrocurstalline Eu2(SO4)3·8H2O and Lu2(SO4)3·8H2O. Previous solubility data for Lu2(SO4)3, at 20 and 40°C, yield an interpolated value at 25°C that is about 30% low. Densities were also measured at several concentrations of each salt. 相似文献
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Dimitrellos V Lamari FN Militsopoulou M Kanakis I Karamanos NK 《Biomedical chromatography : BMC》2003,17(1):42-47
Interaction of basic fibroblast growth factor (bFGF) with heparin/heparan sulfate proteoglycans protects the growth factor against proteolytic degradation and is essential for its cellular activity. Although the structural requirements of heparin and heparan sulfate for the high-affinity binding to bFGF have been extensively examined, studies on intact heparin proteoglycans are limited. In this report, the purity and the binding ability of a heparin proteoglycan-like molecule-the heparin-bovine serum albumin (heparin-BSA) conjugate-was examined using capillary zone electrophoresis (CZE). Furthermore, the affinity of bFGF binding to the heparin-BSA conjugate was studied using an enzyme solid-phase assay. Chondroitin sulfate, dermatan sulfate, hyaluronan, heparan sulfate and variously sulfated disaccharides derived from heparin and heparan sulfate were also studied for their ability to compete with the binding of bFGF to heparin. Heparin-BSA conjugate was synthesized by reductive amination and, following precipitation with 1.5 vols of ethanol-sodium acetate, it was obtained free of contaminating heparin. Heparin-BSA-bFGF conjugate was obtained following incubation of heparin-BSA with bFGF for 2 h at 37 degrees C. Intact heparin, heparin-BSA and heparin-BSA-bFGF conjugates were completely resolved by CZE using 50 mM phosphate, pH 3.5, as operating buffer, reversed polarity (30 kV) and detection at 232 nm. Competitive solid phase assay showed that, among the glycosaminoglycans tested, heparin exhibits the highest affinity binding to bFGF (IC(50) = 6.4 nM). Heparan sulfate showed a lower affinity as compared with that of heparin, whereas all other glycosaminoglycans and heparin/heparan sulfate-derived disaccharides tested showed minute effects. The developed CZE method is rapid and accurate and can be easily used to identify bFGF-interacting heparin preparations of biopharmaceutical importance. 相似文献
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A New Lithium Hydrogen Sulfate, Li2(HSO4)2(H2SO4) – Synthesis and Crystal Structure The title compound crystallizes in good shaped single crystals from the system lithium sulfate/sulfuric acid in the orthorhombic space group Pccn, unit cell parameters a = 17.645(4), b = 5.378(1), c = 10.667(3) Å. V = 1 012.2 Å3, Z = 4, Dx = 2.009 g cm?3. There are two types of SO4 tetrahedra, SO3(OH) and SO2(OH)2, connected via hydrogen bonds forming layers parallel to the xy-plane. The layers are linked by Li atoms, which are tetrahedral coordinated by O atoms coming two by two from neighboured layers. 相似文献