Mixed Supports in the Reversed-Phase Thin-Layer Chromatography of Amino Acids

Abstract

Lipophilicity of amino acids was determined by reversed-phase thin-layer chromatography using silica, aluminium oxide, cellulose, diatomaceous earth and their mixtures as supports with water as eluent.

To compare the retention behaviour of supports principal component analysis /PCA/ was applied. The potency order and the selectivity of support mixtures was calculated by the spectral map technique. Linear and logarithmic correlations were calculated between the first PCA loadings and the potency values as dependent variables and the composition of supports as independent variables.

The first eigenvalue explained more than 90% of the total variance that is only one hidden factor influenced decisively the retention. On the basis of structural differences the retention strength of amino acids on support mixtures can not be explained adequately. The first principal component responsible for the 90% of change in the retention of amino acids is related to the logarithm of support composition that is the sorbents retain their original adsorptive character also after impregnation.     

A Spray Reagent for Identification of Amino Acids on Thin-Layer Chromatography Plates

JPC – Journal of Planar Chromatography – Modern TLC -     

A New Reagent for Identification of Amino Acids on Thin-Layer Chromatography Plates

JPC – Journal of Planar Chromatography – Modern TLC -     

Identification of Amino Acids with Modified Ninhydrin Reagents on Thin-Layer Chromatography Plates

JPC – Journal of Planar Chromatography – Modern TLC - The four new spray reagents (Benzoic acid and some of its parahaloderivatives) have been introduced. These reagents enable...     

Thin-Layer Chromatography of Phenolic Acids on Aminopropylsilica

JPC – Journal of Planar Chromatography – Modern TLC - The possibility of using aminopropylsilica TLC plates for determination of phenolic acids has been investigated. Eighteen phenolic...     

Some Solvent Systems for the Resolution of Dansyl Amino Acids by Silicagel Thin Layer Chromatography

Abstract

The dansyl amino acids have been divided into two groups depending on their chromatographic behaviour on silicagel thin layers in ten new solvent systems, five for each group. The systems reported herein provide rapid and effective resolution (35–45 min) for 20 dansyl amino acids in all. The systems also provide separations of Dns-Thr/Dns-Ser, Dns-Asp/Dns-Glu and Dns-Arg/Dns-∞ lys which were earlier reported [13] to be unresolved even by two-dimensional thin layer chromatography.     

Salting-Out and Salting-in Effects in the Reversed-Phase Thin-Layer Chromatography of Dansylated Amino Acids. Effect of Acids

Abstract

The retention of 15 dansylated amino acid derivatives was determined in aqueous solutions of formic, acetic, propionic and perchloric acid in reversed-phase thin-layer chromatography. The acids increased the retention of each derivatives at the low concentration range. This effect has been tentatively explained by the suppression of dissociation of polar groups in the solute molecules resulting in increased apparent lipophilicity (salting-out phenomenon). The higher concentrations of acid solutions decreased the retention (salting-in effect), the undissociated acid molecules probably act as an organic mobile phase. Both salting-in and salting-out phenomena can be simultaneously described by bilinear function. The polarity parameters of the amino acids, their hydrophobicity and the strength of the acid in the eluent simultaneously influence the retention.     

Identification of Free Amino Acids in Several Crude Extracts of Two Legumes Using Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC -     

氨基酸对映体的手性薄层色谱拆分

设计并制备了３,５－二硝基苯甲酰基取代的β－环糊精薄层色谱键合固定相,用于拆分丹磺酰化氨基酸对映体。该键合相合成方法简单,性能稳定,适用于正相和反相液相色谱。以乙腈－１％（ψ）乙酸三乙胺－乙酸为展开系统,８对丹磺酰化氨基酸对映体得到良好分离,对映异构体之间的相对比移植α为１．１８～１．６３。该文对色谱拆分机理及色谱分离条件进行了较详细的讨论。     

Polyamide Layer Chromatography. Identification of Amino Acids in Angelica acutiloba Kitagawa via DNP Derivative

Eighteen amino acids; proline, alanine, valine, leucine, isoleucine, hydroxyproline, phenylalanine, ornithine, glycine, serine, aspartic acid, glutamic acid, threonine, asparagine, lysine, tyrosine, tryptophan, and arginine were identified by polyamide layer chromatography via DNP (dinitrophenly) derivatives in Angelica acutiloba Kitagawa (Tang-Kwei)     

临床化学上氨基酸、有机酸及核苷类的二维薄层色谱多相分离

在同一块薄层板上多相二维分离３０余种氨基酸、４０余种非挥发性有机酸和２０余种碱基。核苷类化合物。应用一对新的展开剂,用紫外灯－吖啶试剂－镉茚三酮试剂组合显色定位分析,改良了尿液非挥发性有机酸的溶剂萃取。尿液中此三类化合物提取物的多相二维色谱分离效果良好。     

Normal- and Reversed-Phase Thin-Layer Chromatography of Amino Acids on Unconventional Supports with Mixed Mobile Phases Containing Dimethyl Sulfoxide

JPC – Journal of Planar Chromatography – Modern TLC - The chromatographic behavior of twenty-four amino acids has been studied on untreated and on triaryl phosphate (TAP)-impregnated...     

Ion-Pair Reversed-Phase Thin-Layer Chromatography and High-Performance Liquid Chromatography of Benzoic Acids

The separation of benzoic acids by ion-pair reversed-phase thin-layer chromatography and high-performance liquid chromatography was studied. In the optimization of separation conditions, the effect of the nature and concentration of the organic solvent, acidity of the mobile phase, concentration of salts of quaternary ammonium bases, and the length of their hydrocarbon radical on the retention of benzoic acids was studied. Conditions of the separation of a mixture of benzoic, hydroxybenzoic, aminobenzoic, and nitrobenzoic acids on Silufol plates impregnated with cetyltrimethylammonium bromide were selected; the mobile phase was isopropanol–ammonium acetate buffer solution with pH 5–dodecyltrimethylammonium bromide.     

Thin-Layer Chromatography of Bile Alcohols,Bile Acids and Conjugated Bile Acids

Abstract

Thin-layer chromatography of bile alcohols, bile acids and bile acid conjugates has been reviewed. Particular emphasis has been placed on the separation of the glycine and taurine conjugated bile acids as a class and as individual compounds, and on the isolation of bile alcohols and C₂₇ bile acids diastereo-isomeric at C-25.     

Use of Genetic Algorithms and Artificial Neural Networks to Predict the Resolution of Amino Acids in Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC - A novel method is proposed for optimization of simultaneous thinlayer chromatographic separation of seven amino acids. For this...     

Two New Spray Reagents for Detection of Amino Acids on Thin-Layer Plates

JPC – Journal of Planar Chromatography – Modern TLC -     

Planar Chromatography of Free Industrial Amino Acids

JPC – Journal of Planar Chromatography – Modern TLC - Amino acids such as L-serine, L-threonine, L-phenylalanine, L-tryptophan, L-lysine, L-ornithine, L-arginine, L-valine, and...     

用薄层色谱法分离芳香醇胺药物对映体

用D－10-樟脑磷酸铵作为手性离子对试剂添加到流动相中,在硅胶GF254薄层板上分离了两种芳香醇胺类药物对映体。     

Multiple Development in one Dimension of Thin-Layer Chromatograms of Urinary Amino Acids

Abstract

Multiple development in the same direction of thin-layer cellulose chromatograms with n-butanol-acetic acid-water (60:20:20) yielded resolution of 20 ninhydrin-positive spots. The resulting spots were quantitatively estimated by visual comparison with standards.