共查询到20条相似文献，搜索用时 0 毫秒
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《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2017,129(32):9524-9527
Understanding the myriad protein–protein interactions required for cell function requires efficient leveraging of biophysical data to drive computational docking. The detailed insight into protein interfaces provided by isotope exchange endows this experimental technique with a unique importance for docking approaches. However, progress in coupling these methods is hindered by the inability to interpret the complex exchange patterns in relation to protein structure. A method to simulate protein isotope exchange patterns from docking outputs is described and its utility to guide the selection of native assemblies demonstrated. Unique signatures are generated for each docking pose, allowing high‐throughput ranking of whole docking simulations by pairwise comparison to experimental outputs. Native assemblies are obtained using nothing but their simulated profiles as restraints and experimental difference data for individual proteins are sufficient to drive structure determination for the whole complex. 相似文献
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《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2017,129(47):15069-15073
Mapping the interaction sites between membrane‐spanning proteins is a key challenge in structural biology. In this study a carbene‐footprinting approach was developed and applied to identify the interfacial sites of a trimeric, integral membrane protein, OmpF, solubilised in micelles. The diazirine‐based footprinting probe is effectively sequestered by, and incorporated into, the micelles, thus leading to efficient labelling of the membrane‐spanning regions of the protein upon irradiation at 349 nm. Areas associated with protein–protein interactions between the trimer subunits remained unlabelled, thus revealing their location. 相似文献
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Perdita E. Barran 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2011,123(14):3176-3178
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Kathrin Breuker Fred W. McLafferty 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2005,117(31):4989-4992
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《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2017,129(1):281-285
We present an integrated approach for investigating the topology of proteins through native mass spectrometry (MS) and cross‐linking/MS, which we applied to the full‐length wild‐type p53 tetramer. For the first time, the two techniques were combined in one workflow to obtain not only structural insight in the p53 tetramer, but also information on the cross‐linking efficiency and the impact of cross‐linker modification on the conformation of an intrinsically disordered protein (IDP). P53 cross‐linking was monitored by native MS and as such, our strategy serves as a quality control for different cross‐linking reagents. Our approach can be applied to the structural investigation of various protein systems, including IDPs and large protein assemblies, which are challenging to study by the conventional methods used for protein structure characterization. 相似文献
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Chungjung Chou Alexander Deiters 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2011,123(30):6971-6974
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Jin Hae Kim Javier Oroz Markus Zweckstetter 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2016,128(52):16402-16405
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