共查询到20条相似文献,搜索用时 31 毫秒
1.
Nondestructive imaging of the internal microstructure of vessels and nerve fibers in rat spinal cord using phase‐contrast synchrotron radiation microtomography
下载免费PDF全文
![点击此处可从《Journal of synchrotron radiation》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Jianzhong Hu Ping Li Xianzhen Yin Tianding Wu Yong Cao Zhiming Yang Liyuan Jiang Shiping Hu Hongbin Lu 《Journal of synchrotron radiation》2017,24(2):482-489
The spinal cord is the primary neurological link between the brain and other parts of the body, but unlike those of the brain, advances in spinal cord imaging have been challenged by the more complicated and inhomogeneous anatomy of the spine. Fortunately with the advancement of high technology, phase‐contrast synchrotron radiation microtomography has become widespread in scientific research because of its ability to generate high‐quality and high‐resolution images. In this study, this method has been employed for nondestructive imaging of the internal microstructure of rat spinal cord. Furthermore, digital virtual slices based on phase‐contrast synchrotron radiation were compared with conventional histological sections. The three‐dimensional internal microstructure of the intramedullary arteries and nerve fibers was vividly detected within the same spinal cord specimen without the application of a stain or contrast agent or sectioning. With the aid of image post‐processing, an optimization of vessel and nerve fiber images was obtained. The findings indicated that phase‐contrast synchrotron radiation microtomography is unique in the field of three‐dimensional imaging and sets novel standards for pathophysiological investigations in various neurovascular diseases. 相似文献
2.
A novel imaging tool for hepatic portal system using phase contrast technique with hydrogen peroxide‐generated O2 gas
下载免费PDF全文
![点击此处可从《Journal of synchrotron radiation》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Rongbiao Tang Fuhua Yan Wei‐Min Chai Wei Huang Yanan Fu Guo‐Yuan Yang Ke‐Min Chen 《Journal of synchrotron radiation》2015,22(5):1263-1267
The objective of this study was to investigate the potential of hydrogen peroxide‐generated oxygen gas‐based phase contrast imaging (PCI) for visualizing mouse hepatic portal veins. The O2 gas was made from the reaction between H2O2 and catalase. The gas production was imaged by PCI in real time. The H2O2 was injected into the enteric cavity of the lower sigmoid colon to produce O2 in the submucosal venous plexus. The generated O2 gas could be finally drained into hepatic portal veins. Absorption contrast imaging (ACI) and PCI of O2‐filled portal veins were performed and compared. PCI offers high resolution and real‐time visualization of the O2 gas production. Compared with O2‐based ACI, O2‐based PCI significantly enhanced the revealing of the portal vein in vivo. It is concluded that O2‐based PCI is a novel and promising imaging modality for future studies of portal venous disorders in mice models. 相似文献
3.
Potential of propagation‐based synchrotron X‐ray phase‐contrast computed tomography for cardiac tissue engineering
下载免费PDF全文
![点击此处可从《Journal of synchrotron radiation》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Mohammad Izadifar Paul Babyn Dean Chapman Michael E. Kelly Xiongbiao Chen 《Journal of synchrotron radiation》2017,24(4):842-853
Hydrogel‐based cardiac tissue engineering offers great promise for myocardial infarction repair. The ability to visualize engineered systems in vivo in animal models is desired to monitor the performance of cardiac constructs. However, due to the low density and weak X‐ray attenuation of hydrogels, conventional radiography and micro‐computed tomography are unable to visualize the hydrogel cardiac constructs upon their implantation, thus limiting their use in animal systems. This paper presents a study on the optimization of synchrotron X‐ray propagation‐based phase‐contrast imaging computed tomography (PCI‐CT) for three‐dimensional (3D) visualization and assessment of the hydrogel cardiac patches. First, alginate hydrogel was 3D‐printed into cardiac patches, with the pores filled by fibrin. The hydrogel patches were then surgically implanted on rat hearts. A week after surgery, the hearts including patches were excised and embedded in a soft‐tissue‐mimicking gel for imaging by using PCI‐CT at an X‐ray energy of 25 keV. During imaging, the sample‐to‐detector distances, CT‐scan time and the region of interest (ROI) were varied and examined for their effects on both imaging quality and radiation dose. The results showed that phase‐retrieved PCI‐CT images provided edge‐enhancement fringes at a sample‐to‐detector distance of 147 cm that enabled visualization of anatomical and microstructural features of the myocardium and the implanted patch in the tissue‐mimicking gel. For visualization of these features, PCI‐CT offered a significantly higher performance than the dual absorption‐phase and clinical magnetic resonance (3 T) imaging techniques. Furthermore, by reducing the total CT‐scan time and ROI, PCI‐CT was examined for lowering the effective dose, meanwhile without much loss of imaging quality. In effect, the higher soft tissue contrast and low‐dose potential of PCI‐CT has been used along with an acceptable overall animal dose to achieve the high spatial resolution needed for cardiac implant visualization. As a result, PCI‐CT at the identified imaging parameters offers great potential for 3D assessment of microstructural features of hydrogel cardiac patches. 相似文献
4.
Lei Tang Gang Li Ying‐Shi Sun Jie Li Xiao‐Peng Zhang 《Journal of synchrotron radiation》2012,19(3):319-322
The electron density resolution of synchrotron‐radiation phase‐contrast imaging (SR‐PCI) is 1000 times higher than that of conventional X‐ray absorption imaging in light elements, through which high‐resolution X‐ray imaging of biological soft tissue can be achieved. For biological soft tissue, SR‐PCI can give better imaging contrast than conventional X‐ray absorption imaging. In this study, human resected stomach and gastric cancer were investigated using in‐line holography and diffraction enhanced imaging at beamline 4W1A of the Beijing Synchrotron Radiation Facility. It was possible to depict gastric pits, measuring 50–70 µm, gastric grooves and tiny blood vessels in the submucosa layer by SR‐PCI. The fine structure of a cancerous ulcer was displayed clearly on imaging the mucosa. The delamination of the gastric wall and infiltration of cancer in the submucosa layer were also demonstrated on cross‐sectional imaging. In conclusion, SR‐PCI can demonstrate the subtle structures of stomach and gastric cancer that cannot be detected by conventional X‐ray absorption imaging, which prompt the X‐ray diagnosis of gastric disease to the level of the gastric pit, and has the potential to provide new methods for the imageology of gastric cancer. 相似文献
5.
Design,development and first experiments on the X‐ray imaging beamline at Indus‐2 synchrotron source RRCAT,India
下载免费PDF全文
![点击此处可从《Journal of synchrotron radiation》网站下载免费的PDF全文](/ch/ext_images/free.gif)
A. K. Agrawal B. Singh Y. S. Kashyap M. Shukla P. S. Sarkar Amar Sinha 《Journal of synchrotron radiation》2015,22(6):1531-1539
A full‐field hard X‐ray imaging beamline (BL‐4) was designed, developed, installed and commissioned recently at the Indus‐2 synchrotron radiation source at RRCAT, Indore, India. The bending‐magnet beamline is operated in monochromatic and white beam mode. A variety of imaging techniques are implemented such as high‐resolution radiography, propagation‐ and analyzer‐based phase contrast imaging, real‐time imaging, absorption and phase contrast tomography etc. First experiments on propagation‐based phase contrast imaging and micro‐tomography are reported. 相似文献
6.
Patrik Vagovič Dušan Korytár Petr Mikulík Angelica Cecilia Claudio Ferrari Yang Yang Daniel Hänschke Elias Hamann Daniele Pelliccia Tamzin A. Lafford Michael Fiederle Tilo Baumbach 《Journal of synchrotron radiation》2011,18(5):753-760
In this work an X‐ray imaging system based on a recently developed in‐line two‐dimensional Bragg magnifier composed of two monolithic V‐shaped crystals made of dislocation‐free germanium is presented. The channel‐cut crystals were used in one‐dimensional and in two‐dimensional (crossed) configurations in imaging applications and allowed measurement of phase‐contrast radiograms both in the edge‐enhanced and in the holographic regimes. The measurement of the phase gradient in two orthogonal directions is demonstrated. The effective pixel size attained was 0.17 µm in the one‐dimensional configuration and 0.5 µm in the two‐dimensional setting, offering a twofold improvement in spatial resolution over devices based on silicon. These results show the potential for applying Bragg magnifiers to imaging soft matter at high resolution with reduced dose owing to the higher efficiency of Ge compared with Si. 相似文献
7.
Florian Meirer Jordi Cabana Yijin Liu Apurva Mehta Joy C. Andrews Piero Pianetta 《Journal of synchrotron radiation》2011,18(5):773-781
The ability to probe morphology and phase distribution in complex systems at multiple length scales unravels the interplay of nano‐ and micrometer‐scale factors at the origin of macroscopic behavior. While different electron‐ and X‐ray‐based imaging techniques can be combined with spectroscopy at high resolutions, owing to experimental time limitations the resulting fields of view are too small to be representative of a composite sample. Here a new X‐ray imaging set‐up is proposed, combining full‐field transmission X‐ray microscopy (TXM) with X‐ray absorption near‐edge structure (XANES) spectroscopy to follow two‐dimensional and three‐dimensional morphological and chemical changes in large volumes at high resolution (tens of nanometers). TXM XANES imaging offers chemical speciation at the nanoscale in thick samples (>20 µm) with minimal preparation requirements. Further, its high throughput allows the analysis of large areas (up to millimeters) in minutes to a few hours. Proof of concept is provided using battery electrodes, although its versatility will lead to impact in a number of diverse research fields. 相似文献
8.
Tohoru Takeda Akio Yoneyama Jin Wu Atsushi Momose Kazuyuki Hyodo 《Journal of synchrotron radiation》2012,19(2):252-256
Using a two‐crystal‐interferometer‐based phase‐contrast X‐ray imaging system, the portal vein, capillary vessel area and hepatic vein of live rats were revealed sequentially by injecting physiological saline via the portal vein. Vessels greater than 0.06 mm in diameter were clearly shown with low levels of X‐rays (552 µGy). This suggests that in vivo vessel imaging of small animals can be performed as conventional angiography without the side effects of the presently used iodine contrast agents. 相似文献
9.
Rhiannon P. Murrie Andrew W. Stevenson Kaye S. Morgan Andreas Fouras David M. Paganin Karen K. W. Siu 《Journal of synchrotron radiation》2014,21(2):430-445
Propagation‐based phase‐contrast X‐ray imaging (PB‐PCXI) using synchrotron radiation has achieved high‐resolution imaging of the lungs of small animals both in real time and in vivo. Current studies are applying such imaging techniques to lung disease models to aid in diagnosis and treatment development. At the Australian Synchrotron, the Imaging and Medical beamline (IMBL) is well equipped for PB‐PCXI, combining high flux and coherence with a beam size sufficient to image large animals, such as sheep, due to a wiggler source and source‐to‐sample distances of over 137 m. This study aimed to measure the capabilities of PB‐PCXI on IMBL for imaging small animal lungs to study lung disease. The feasibility of combining this technique with computed tomography for three‐dimensional imaging and X‐ray velocimetry for studies of airflow and non‐invasive lung function testing was also investigated. Detailed analysis of the role of the effective source size and sample‐to‐detector distance on lung image contrast was undertaken as well as phase retrieval for sample volume analysis. Results showed that PB‐PCXI of lung phantoms and mouse lungs produced high‐contrast images, with successful computed tomography and velocimetry also being carried out, suggesting that live animal lung imaging will also be feasible at the IMBL. 相似文献
10.
The 3D characteristics of post‐traumatic syringomyelia in a rat model: a propagation‐based synchrotron radiation microtomography study
下载免费PDF全文
![点击此处可从《Journal of synchrotron radiation》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Shenghui Liao Shuangfei Ni Yong Cao Xianzhen Yin Tianding Wu Hongbin Lu Jianzhong Hu Hao Wu Ye Lang 《Journal of synchrotron radiation》2017,24(6):1218-1225
Many published literature sources have described the histopathological characteristics of post‐traumatic syringomyelia (PTS). However, three‐dimensional (3D) visualization studies of PTS have been limited due to the lack of reliable 3D imaging techniques. In this study, the imaging efficiency of propagation‐based synchrotron radiation microtomography (PB‐SRµCT) was determined to detect the 3D morphology of the cavity and surrounding microvasculature network in a rat model of PTS. The rat model of PTS was established using the infinite horizon impactor to produce spinal cord injury (SCI), followed by a subarachnoid injection of kaolin to produce arachnoiditis. PB‐SRµCT imaging and histological examination, as well as fluorescence staining, were conducted on the animals at the tenth week after SCI. The 3D morphology of the cystic cavity was vividly visualized using PB‐SRµCT imaging. The quantitative parameters analyzed by PB‐SRµCT, including the lesion and spared spinal cord tissue area, the minimum and maximum diameters in the cystic cavity, and cavity volume, were largely consistent with the results of the histological assessment. Moreover, the 3D morphology of the cavity and surrounding angioarchitecture could be simultaneously detected on the PB‐SRµCT images. This study demonstrated that high‐resolution PB‐SRµCT could be used for the 3D visualization of trauma‐induced spinal cord cavities and provides valuable quantitative data for cavity characterization. PB‐SRµCT could be used as a reliable imaging technique and offers a novel platform for tracking cavity formation and morphological changes in an experimental animal model of PTS. 相似文献
11.
Microbubbles containing gadolinium as contrast agents for both phase contrast and magnetic resonance imaging
下载免费PDF全文
![点击此处可从《Journal of synchrotron radiation》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Rongbiao Tang Fuhua Yan Guo-Yuan Yang Ke-Min Chen 《Journal of synchrotron radiation》2018,25(2):560-564
Portal vein imaging is an important method for investigating portal venous disorders. However, the diagnostic requirements are not usually satisfied when using single imaging techniques. Diagnostic accuracy can be improved by combining different imaging techniques. Contrast agents that can be used for combined imaging modalities are needed. In this study, the feasibility of using microbubbles containing gadolinium (MCG) as contrast agents for both phase contrast imaging (PCI) and magnetic resonance imaging (MRI) are investigated. MCG were made by encapsulating sulfur hexafluoride (SF6) gas with gadolinium and lyophilized powder. Absorption contrast imaging (ACI) and PCI of MCG were performed and compared in vitro. MCG were injected into the main portal trunk of living rats. PCI and MRI were performed at 2 min and 10 min after MCG injection, respectively. PCI exploited the differences in the refractive index and visibly showed the MCG, which were not detectable by ACI. PCI could facilitate clear revelation of the MCG‐infused portal veins. The diameter of the portal veins could be determined by the largest MCG in the same portal vein. The minimum diameter of clearly detected portal veins was about 300 µm by MRI. These results indicate that MCG could enhance both PCI and MRI for imaging portal veins. The detection sensitivity of PCI and MRI could compensate for each other when using MCG contrast agents for animals. 相似文献
12.
A feasibility study of X‐ray phase‐contrast mammographic tomography at the Imaging and Medical beamline of the Australian Synchrotron
下载免费PDF全文
![点击此处可从《Journal of synchrotron radiation》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Yakov I. Nesterets Timur E. Gureyev Sheridan C. Mayo Andrew W. Stevenson Darren Thompson Jeremy M. C. Brown Marcus J. Kitchen Konstantin M. Pavlov Darren Lockie Francesco Brun Giuliana Tromba 《Journal of synchrotron radiation》2015,22(6):1509-1523
Results are presented of a recent experiment at the Imaging and Medical beamline of the Australian Synchrotron intended to contribute to the implementation of low‐dose high‐sensitivity three‐dimensional mammographic phase‐contrast imaging, initially at synchrotrons and subsequently in hospitals and medical imaging clinics. The effect of such imaging parameters as X‐ray energy, source size, detector resolution, sample‐to‐detector distance, scanning and data processing strategies in the case of propagation‐based phase‐contrast computed tomography (CT) have been tested, quantified, evaluated and optimized using a plastic phantom simulating relevant breast‐tissue characteristics. Analysis of the data collected using a Hamamatsu CMOS Flat Panel Sensor, with a pixel size of 100 µm, revealed the presence of propagation‐based phase contrast and demonstrated significant improvement of the quality of phase‐contrast CT imaging compared with conventional (absorption‐based) CT, at medically acceptable radiation doses. 相似文献
13.
Visualization of microvasculature and thrombi by X‐ray phase‐contrast computed tomography in hepatocellular carcinoma
下载免费PDF全文
![点击此处可从《Journal of synchrotron radiation》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Jianbo Jian Hao Yang Xinyan Zhao Ruijiao Xuan Yujie Zhang Dongyue Li Chunhong Hu 《Journal of synchrotron radiation》2016,23(2):600-605
Visualization of the microvascular network and thrombi in the microvasculature is a key step to evaluating the development of tumor growth and metastasis, and influences treatment selection. X‐ray phase‐contrast computed tomography (PCCT) is a new imaging technique that can detect minute changes of density and reveal soft tissues discrimination at micrometer‐scale resolution. In this study, six human resected hepatocellular carcinoma (HCC) tissues were investigated with PCCT. A histological stain was added to estimate the accuracy of PCCT. The results showed that the fine structures of the microvasculature (measuring 30–100 µm) and thrombi in tiny blood vessels were displayed clearly on imaging the HCC tissues by PCCT. Moreover, density distributions of the thrombi were obtained, which could be reliably used to distinguish malignant from benign thrombi in HCC. In conclusion, PCCT can clearly show the three‐dimensional subtle structures of HCC that cannot be detected by conventional absorption‐based computed tomography and provides a new method for the imageology of HCC. 相似文献
14.
Using synchrotron radiation inline phase‐contrast imaging computed tomography to visualize three‐dimensional printed hybrid constructs for cartilage tissue engineering
下载免费PDF全文
![点击此处可从《Journal of synchrotron radiation》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Adeola D. Olubamiji Zohreh Izadifar Ning Zhu Tuanjie Chang Xiongbiao Chen B. Frank Eames 《Journal of synchrotron radiation》2016,23(3):802-812
Synchrotron radiation inline phase‐contrast imaging combined with computed tomography (SR‐inline‐PCI‐CT) offers great potential for non‐invasive characterization and three‐dimensional visualization of fine features in weakly absorbing materials and tissues. For cartilage tissue engineering, the biomaterials and any associated cartilage extracellular matrix (ECM) that is secreted over time are difficult to image using conventional absorption‐based imaging techniques. For example, three‐dimensional printed polycaprolactone (PCL)/alginate/cell hybrid constructs have low, but different, refractive indices and thicknesses. This paper presents a study on the optimization and utilization of inline‐PCI‐CT for visualizing the components of three‐dimensional printed PCL/alginate/cell hybrid constructs for cartilage tissue engineering. First, histological analysis using Alcian blue staining and immunofluorescent staining assessed the secretion of sulfated glycosaminoglycan (GAGs) and collagen type II (Col2) in the cell‐laden hybrid constructs over time. Second, optimization of inline PCI‐CT was performed by investigating three sample‐to‐detector distances (SDD): 0.25, 1 and 3 m. Then, the optimal SDD was utilized to visualize structural changes in the constructs over a 42‐day culture period. The results showed that there was progressive secretion of cartilage‐specific ECM by ATDC5 cells in the hybrid constructs over time. An SDD of 3 m provided edge‐enhancement fringes that enabled simultaneous visualization of all components of hybrid constructs in aqueous solution. Structural changes that might reflect formation of ECM also were evident in SR‐inline‐PCI‐CT images. Summarily, SR‐inline‐PCI‐CT images captured at the optimized SDD enables visualization of the different components in hybrid cartilage constructs over a 42‐day culture period. 相似文献
15.
Ismo Vartiainen Martin Warmer Dennis Goeries Eva Herker Rudolph Reimer Christian David Alke Meents 《Journal of synchrotron radiation》2014,21(4):790-794
X‐ray microscopy is a commonly used method especially in material science application, where the large penetration depth of X‐rays is necessary for three‐dimensional structural studies of thick specimens with high‐Z elements. In this paper it is shown that full‐field X‐ray microscopy at 6.2 keV can be utilized for imaging of biological specimens with high resolution. A full‐field Zernike phase‐contrast microscope based on diffractive optics is used to study lipid droplet formation in hepatoma cells. It is shown that the contrast of the images is comparable with that of electron microscopy, and even better contrast at tender X‐ray energies between 2.5 keV and 4 keV is expected. 相似文献
16.
Rongbiao Tang Fuhua Yan Guo-Yuan Yang Ke-Min Chen 《Journal of synchrotron radiation》2017,24(6):1260-1264
Preoperative portal vein embolization (PVE) is employed clinically to avoid postoperative liver insufficiency. Animal models are usually used to study PVE in terms of mechanisms and pathophysiological changes. PVE is formerly monitored by conventional absorption contrast imaging (ACI) with iodine contrast agent. However, the side effects induced by iodine can give rise to animal damage and death. In this study, the feasibility of using phase contrast imaging (PCI) to show PVE using homemade CO2 microbubbles in living rats has been investigated. CO2 gas was first formed from the reaction between citric acid and sodium bicarbonate. The CO2 gas was then encapsulated by egg white to fabricate CO2 microbubbles. ACI and PCI of CO2 microbubbles were performed and compared in vitro. An additional increase in contrast was detected in PCI. PCI showed that CO2 microbubbles gradually dissolved over time, and the remaining CO2 microbubbles became larger. By PCI, the CO2 microbubbles were found to have certain stability, suggesting their potential use as embolic agents. CO2 microbubbles were injected into the main portal trunk to perform PVE in living rats. PCI exploited the differences in the refractive index and facilitated clear visualization of the PVE after the injection of CO2 microbubbles. Findings from this study suggest that homemade CO2 microbubbles‐based PCI is a novel modality for preclinical PVE research. 相似文献
17.
A three‐dimensional X‐ray detector for imaging 30–200 keV photons is described. It comprises a set of semi‐transparent structured scintillators, where each scintillator is a regular array of waveguides in silicon, and with pores filled with CsI. The performance of the detector is described theoretically and explored in detail through simulations. Based on available hardware, a spatial resolution of 1 µm is obtainable. The resolution of a single screen is shown to be determined only by the pitch, at least up to 100 keV. In comparison with conventional homogeneous screens, an improvement in efficiency by a factor of 5–15 is obtainable. The cross‐talk between screens in the three‐dimensional detector is shown to be negligible. The three‐dimensional concept enables ray‐tracing and super‐resolution algorithms to be applied. 相似文献
18.
Vishnu Vardhan Pully Aufried Lenferink Cees Otto 《Journal of Raman spectroscopy : JRS》2010,41(6):599-608
A hybrid fluorescence–Raman confocal microscopy platform is presented, which integrates low‐wavenumber‐resolution Raman imaging, Rayleigh scatter imaging and two‐photon fluorescence (TPE) spectral imaging, fast ‘amplitude‐only’ TPE‐fluorescence imaging and high‐spectral‐resolution Raman imaging. This multi‐dimensional fluorescence–Raman microscopy platform enables rapid imaging along the fluorescence emission and/or Rayleigh scatter dimensions. It is shown that optical contrast in these images can be used to select an area of interest prior to subsequent investigation with high spatially and spectrally resolved Raman imaging. This new microscopy platform combines the strengths of Raman ‘chemical’ imaging with light scattering microscopy and fluorescence microscopy and provides new modes of correlative light microscopy. Simultaneous acquisition of TPE hyperspectral fluorescence imaging and Raman imaging illustrates spatial relationships of fluorophores, water, lipid and protein in cells. The fluorescence–Raman microscope is demonstrated in an application to living human bone marrow stromal stem cells. Copyright © 2009 John Wiley & Sons, Ltd. 相似文献
19.
Synchrotron X‐ray phase contrast imaging of leaf venation in soybean (Glycine max) after exclusion of solar UV (280–400 nm) radiation
下载免费PDF全文
![点击此处可从《Journal of synchrotron radiation》网站下载免费的PDF全文](/ch/ext_images/free.gif)
A. Fatima S. Kataria K. N. Guruprasad A. K. Agrawal B. Singh P. S. Sarkar T. Shripathi Y. Kashyap A. Sinha 《Journal of synchrotron radiation》2016,23(3):795-801
The hydraulic efficiency of a leaf depends on its vascular structure as this is responsible for transport activities. To investigate the effect of exclusion of UVAB and UVB radiation from the solar spectrum on the micro‐structure of leaves of soybean (Glycine max, variety JS‐335), a field experiment was conducted using synchrotron‐based phase contrast imaging (PCI). Plants were grown in specially designed UV exclusion chambers, and wrapped with filters that excluded UVB (280–315 nm) or UVAB (280–400 nm), or transmitted all the ambient solar UV (280–400 nm) radiation (filter control). Qualitative observation of high‐resolution X‐ray PCI images obtained at 10 keV has shown the differences in major and minor vein structures of the leaves. The mid‐rib width of the middle leaflet of third trifoliate leaves, for all treatments, were obtained using quantitative image analysis. The width of the mid‐rib of the middle leaflet of third trifoliate leaves of UVB excluded plants was found to be more compared to leaves of filter control plants, which are exposed to ambient UV. The mid‐rib or the main conducting vein transports water and sugars to the whole plant; therefore, mid‐rib enhancement by the exclusion of solar UV radiation possibly implies enhancement in the leaf area which in turn causes an increased rate of photosynthesis. 相似文献
20.
Live small‐animal X‐ray lung velocimetry and lung micro‐tomography at the Australian Synchrotron Imaging and Medical Beamline
下载免费PDF全文
![点击此处可从《Journal of synchrotron radiation》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Rhiannon P. Murrie Kaye S. Morgan Anton Maksimenko Andreas Fouras David M. Paganin Chris Hall Karen K. W. Siu David W. Parsons Martin Donnelley 《Journal of synchrotron radiation》2015,22(4):1049-1055
The high flux and coherence produced at long synchrotron beamlines makes them well suited to performing phase‐contrast X‐ray imaging of the airways and lungs of live small animals. Here, findings of the first live‐animal imaging on the Imaging and Medical Beamline (IMBL) at the Australian Synchrotron are reported, demonstrating the feasibility of performing dynamic lung motion measurement and high‐resolution micro‐tomography. Live anaesthetized mice were imaged using 30 keV monochromatic X‐rays at a range of sample‐to‐detector propagation distances. A frame rate of 100 frames s?1 allowed lung motion to be determined using X‐ray velocimetry. A separate group of humanely killed mice and rats were imaged by computed tomography at high resolution. Images were reconstructed and rendered to demonstrate the capacity for detailed, user‐directed display of relevant respiratory anatomy. The ability to perform X‐ray velocimetry on live mice at the IMBL was successfully demonstrated. High‐quality renderings of the head and lungs visualized both large structures and fine details of the nasal and respiratory anatomy. The effect of sample‐to‐detector propagation distance on contrast and resolution was also investigated, demonstrating that soft tissue contrast increases, and resolution decreases, with increasing propagation distance. This new capability to perform live‐animal imaging and high‐resolution micro‐tomography at the IMBL enhances the capability for investigation of respiratory diseases and the acceleration of treatment development in Australia. 相似文献