共查询到17条相似文献,搜索用时 46 毫秒
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毛细管电色谱研究进展 总被引:7,自引:2,他引:7
毛细管电色谱是一种新兴的具有高效,高选择性的微分离技术。本文评述了毛细管电色说的发展状况和相关的技术,并对其发展前景进行了展望,引用文献24篇 。 相似文献
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毛细管电色谱技术(CEC)是一种集微柱液相色谱的高选择性、多样性及高效毛细管电泳(HPCE)的高效性、高分辩率为一体的新的电泳分离技术。本文介绍了毛细管电色谱的历史、发展及研究现状;并对电色谱的基本理论与技术作了阐述。 相似文献
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毛细管离子交换电色谱的分离行为 总被引:7,自引:0,他引:7
在离子交换毛细管色谱柱上实施电色谱,并对其分离行为进行了研究,采用75μm(i.d.)20cm的毛细管强阳离子交换柱(3μm)以NaH2PO4-H3PO4缓冲液为淋洗剂,紫外柱上检测(214nm)考察了流动相的pH值,有机改性剂及分离电压等因素对分离的影响,研究表明,不同的pH溶质的流出次序发生改变,随着有机改性剂含量增加,溶质的保留时间减小,而电渗流却增大,同时,对分离的柱效和方法的重现性进行了 相似文献
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手性化合物的分离分析一直是分析化学领域的一个重要研究课题.对映异构体在非手性环境中具有相同的化学和物理性质,但在生物体系中会展现出不同的生物和生理活性.因此,手性对映体的有效拆分对于医药、生物领域有着重大的意义.本文就近年来毛细管电色谱技术及其对手性化合物的拆分研究进行归纳及综述,介绍了基于不同材料,包括多糖衍生物、纳... 相似文献
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An improved technique, pressurized capillary electrochromatography (pCEC) coupling with end-column amperometric detection (AD), was developed and used for the separation and determination of estrogens. The effects of pH value, composition of mobile phase, concentration of the surfactant sodium dodecyl sulfate (SDS) and applied voltage on separation were investigated. The electrochemical oxidation of diethylstilbestrol (DES), dienestrol (DE), and hexestrol (HEX) could be reliably monitored with a carbon electrode at 0.9 V (vs. Ag/AgCl). The pCEC analyses were performed on a capillary separation column packed with 3 microm C18 particles with an acetonitrile/water (31%: 69%) mobile phase containing Tris buffer (5 mmol/L, pH 4.5) and 4 mmol/L SDS. High voltage up to 12 kV reduced the retention time dramatically and still provided a baseline resolution. In addition, supplementary pressure prevented bubble formation and provided reliability and reproducibility of the pCEC performance. The detection limits for the three estrogens ranged from 1.2 to 2.2x10(-7) mol/L, about 10 20-fold lower than those obtained with pCEC-UV detection. To evaluate the feasibility and reliability of this system, the proposed pCEC-AD method was further demonstrated with fish muscle samples spiked with estrogens. 相似文献
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Nonporous silica spheres (1 microm) were synthesized and bonded with octadecylsilane functionality. These stationary phase particles were packed electrokinetically into fused-silica capillaries with 100 microm id for a length of 20 cm, which was evaluated by using pressurized CEC (pCEC). The efficiency of the C18 RP column was characterized through the theoretical plates of thiourea, benzyl alcohol, toluene, styrene, and naphthalene. The effects of experimental parameters such as the applied voltage, sample size, pump flow rate, pH value and the concentration of the buffer solution, and the content of methanol in the mobile phase, on-column efficiency were evaluated. Column efficiency as high as 200 000 theoretical plates per meter for naphthalene was obtained with the optimal condition of 70% v/v methanol and 30% v/v of 10 mmol/L phosphate buffer (pH 7.8) at an applied voltage of 10 kV and a supplementary pressure of 500 psi. 相似文献
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Lin‐Feng Hu Shi‐Jun Yin Hao Zhang Feng‐Qing Yang 《Journal of separation science》2020,43(9-10):1942-1966
Capillary electrochromatography, which combined the high selectivity of high‐performance liquid chromatography and the high separation efficiency of capillary electrophoresis, is an attractive separation tool. In this review, the developments on monolithic and open tubular capillary electrochromatography during 2017 to August 2019 are summarized. Considering the development of novel stationary phases is the most active research field in capillary electrochromatography, monolithic capillary electrochromatography is classified according to the polymer‐based and hybrid monolithic columns, while open‐tubular capillary electrochromatography is categorized by cyclodextrin, silica, polymer, nanomaterials, microporous materials, and biomaterials‐based open tubular columns. 相似文献
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